A nano antibacterial agent for killing drug-resistant bacteria and preparation method thereof
A nano-antibacterial agent and drug-resistant bacteria technology, which is applied in the fields of pharmaceutical formulations, medical preparations containing active ingredients, drug delivery, etc., can solve the problems of unsafe laser power density, complex preparation process of nanomaterials, and low antibacterial activity. Achieve broad market application prospects, improve biological safety, and achieve the effect of low dosage
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Embodiment 1
[0030] Example 1, Cu 2 MOS 4 Preparation of nanomaterials
[0031] 0.5-5 g of Cu 2 MOS 4 Materials and 5-50 g of polyvinylpyrrolidone (molecular weights 8-360 k) in the ball mill, providing a ball milling speed of 500 rpm, ball milling time of 12 h. After the ball mill, the centrifugation speed was selected from 12,000 rpm, the centrifugal time was 1 H gradient centrifugal condition purified nanomaterial, and finally dispersed in ultrapure water, stored in a 4 ° C refrigerator, standby.
Embodiment 2
[0032] Example 2, Cu 2 MOS 4 Biosafety test of nanomaterials
[0033] 1. Use fresh mouse blood research Cu 2 MOS 4 Blood compatibility of nanomaterials.
[0034] After adding 3-10 mL of physiological saline, 3-10 ml of physiological saline is added to the centrifuge tube with fresh blood, and the centrifuge tube is placed in the centrifuge, and blood red blood cells are extracted using saline purification.
[0035] Pure 0.1 ~ 1 mL of hemodern cells were then dissolved in 5 to 10 ml of physiological saline. 1 ml of different concentrations in multi-tube 2 MOS 4 0.01 to 0.8 mL of hemodern cells were added to the saline solution of nanomaterials, and after mixing at 37 ° C, the rotational speed was 50 to 200 rpm, incubated 0.5 to 10 h.
[0036] from figure 1 It can be seen in Cu. 2 MOS 4 Nanomaterials have good blood compatibility.
[0037] 2. Study using Hela Cells Cu 2 MOS 4 Nam material cytotoxicity.
[0038] HELA cells (1-6 × 10 4 ) With different concentrations of Cu 2 MOS 4 Nan...
Embodiment 3
[0040] Example 3, Cu 2 MOS 4 Nanomaterials in vitro antibacterial properties
[0041]Chloromycin, streptomycin and tetracycline E. coli (Gram-negative bacteria) and Golden Staphylococcus aureus (Gram-positive bacteria) of methylene-resistant Western Toplin (Gram-positive bacteria) are stored in glycerol in 5 ~ 30%, preserved The -80 ° C refrigerator.
[0042] Part of the freeze-containing solution was applied using a sterile gun head, scratching on the LB plate, placed under 37 ° C, and cultured overnight, forming the colony visible in the eye.
[0043] A single colon was collected in LB (1 to 20 mL) culture solution using a gun head, shock overnight (220 rpm, 37 ° C).
[0044] 1 mL overnight bacterial suspension was centrifuged in 1.5 ml of centrifuge, from 5,000 to 12000 rpm 1 to 5 min, and saline washed twice. The final centrifugal product was added to 1 ml of physiological saline.
[0045] The 200 μl suspension was taken in the 96-well plate, and the enzyme gauge meter measure...
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