A multi-biochar-combined microbial composite material for oil-contaminated soil remediation and its preparation method and application
A technology for oil pollution and soil remediation, applied in the field of oil-contaminated soil remediation materials, can solve the problems of single species, low biomass and the like, and achieve the effects of simple preparation method, wide source, and favorable for large-scale production and application.
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Embodiment 1
[0057] S1: Preparation of biochar: Tea processing waste, sugar cane and traditional Chinese medicine residues were anaerobically burned at 400°C, 600°C and 300°C, respectively, and soaked in 0.1M HCl solution for 24 hours after cooling to room temperature. To remove the ash, wash the biochar with deionized water until it is neutral, and finally dry it at 60°C to form a powder, and mix the three powders evenly to obtain a mixed biochar powder;
[0058] S2: Culture of microorganisms: Inoculate and activate five microbial strains including Pseudomonas, Achromobacter, Bacillus subtilis, penicillin and Aspergillus, use CN medium for Pseudomonas, use LB for Achromobacter and Bacillus subtilis Medium, Penicillium, Aspergillus use PDA medium to expand culture to the stationary phase. Rinse the collected bacterial cells with the sterilized NaCl solution, and prepare bacterial suspensions of 5 different bacteria (specifically follow the steps recorded in the instructions). Then 5 kinds...
Embodiment 2
[0063] S1: the steps are consistent with Example 1;
[0064] S2: Mix the two fungal suspensions to obtain a petroleum hydrocarbon fungal degradation agent, and the number of active bacteria is not less than 1×10 7 cfu / mL, and then carry out the steps of S3 with 1g tea biochar, 1g sugarcane biochar powder, 1g traditional Chinese medicine biochar and 1g mixed biochar powder in S1 respectively to obtain 4 different biochars, which are put into the oil-contaminated soil pile , to monitor changes in petroleum hydrocarbons and heavy metals in contaminated soil 7-21 days after implementation.
[0065] The experiment found that although the mixed biochar has a strong effect of adsorbing heavy metals, it has a weak effect of degrading petroleum hydrocarbons. On the whole, the biochar mixed with fungal suspension and sugarcane biochar powder has better adsorption and degradation effects than the other three.
Embodiment 3
[0067] The difference from Example 2 is that the two fungal suspensions were replaced by three bacterial suspensions, and the rest of the steps were the same to obtain 4 different biochars.
[0068] The experiment found that the biochar made by mixing the bacterial suspension and the tea biochar powder had a better effect of adsorbing and degrading pollutants than the other three.
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