Biological stabilizer for inhibiting corrosion of metal working fluid, and preparation method and application thereof
A metalworking fluid and bio-stabilization technology, applied in the petroleum industry, lubricating compositions, etc., can solve problems such as damage to the health of users in the environment, and achieve the effect of prolonging the service life and wide application
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Embodiment 1、2 and comparative example 1
[0045] One, embodiment 1,2 and comparative example 1,2 source of raw material
[0046] Galactose, biotin and niacin, glycerol, and sodium glutamate are all Aladdin reagents.
[0047] Diethanolamine and boric acid ester are selected from Jiangsu Haian Petrochemical Plant.
[0048] The liquid medium is liquid LB medium, which was purchased from Beijing Spectrum Technology Co., Ltd.
[0049] Trichoderma was selected as Trichoderma harzianum, numbered BNCC336568, purchased from Beina Biology.
[0050]The genus Pseudomonas was Pseudomonas aeruginosa, numbered BNCC336458, which was purchased from Beina Biotech.
[0051] 2. Preparation method
[0052] 1. Preparation of protobacteria powder thallus
[0053] The preparation of protobacteria powder thalline comprises the following steps:
[0054] S1: Extract the activated protobacteria and inoculate them into an appropriate amount of protobacteria liquid medium (the volume ratio of activated protobacteria to liquid medium is 1:100,...
Embodiment 1、2
[0065] Table 2 embodiment 1,2 and comparative example 1,2 biostabilizer preparation process condition selection
[0066]
[0067] It should be noted that when preparing with reference to the preparation method of the biological stabilizer described in the present invention, the biological stabilizer in Comparative Example 1 and Comparative Example 2 can be correspondingly increased or decreased in raw materials according to the actual formula composition.
[0068] Three, the biological stabilizer of embodiment 1,2 and comparative example 1,2
[0069] Prepare the native bacterial powder thalline and biostabilizer of embodiment 1,2 and comparative example 1,2 according to the above-mentioned method, the composition of the biostabilizer described in embodiment 1,2 and comparative example 1,2 is shown in Table 3.
[0070] The composition of biological stabilizer described in table 3 embodiment 1,2 and comparative example 1,2
[0071]
[0072] 4. Detection method
[0073] (...
Embodiment 1
[0079] Example 1-1: The self-developed emulsified metalworking fluid TSIcut-Emu1000Ti removes the bactericide component, and adds 3% (wt) of the biological stabilizer described in Example 1.
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