A strain of pseudomonas graminis capable of degrading cellulose at low temperature and its application

A cellulose and potassium decomposing technology, applied to a Pseudomonas graminis bacterium capable of degrading cellulose at low temperature and its application field, can solve problems such as limited application range, and achieve the effects of improving soil fertility, promoting effect, and promoting straw maturity

Active Publication Date: 2022-04-05
BEIJING ZNFY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] To sum up, the current research on low-temperature cellulose-degrading bacteria is mostly focused on 10°C-20°C. Although it brings certain advantages, the scope of application is still relatively limited.

Method used

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  • A strain of pseudomonas graminis capable of degrading cellulose at low temperature and its application
  • A strain of pseudomonas graminis capable of degrading cellulose at low temperature and its application
  • A strain of pseudomonas graminis capable of degrading cellulose at low temperature and its application

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Embodiment Construction

[0075] The inventor screened a large number of soils from all over the country, and screened out a strain from nearly 300,000 microbial strains that can grow stably and rapidly at a low temperature of 4°C and is relatively stable in reducing cellulose, and can also be used at medium and high temperatures The strain that degrades cellulose is named KY240. In addition, the strain also has the functions of degrading mineral potassium and inorganic phosphorus.

[0076] Determination of 16sDNA of KY240 strain and analysis of strain physiological morphology:

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Abstract

The invention discloses a Pseudomonas graminis bacterium capable of degrading cellulose at low temperature and its application, and its preservation number is CGMCC NO.: 18751. The strain has a strong ability to degrade cellulose at a low temperature of 4°C and 10°C, and the ability to degrade cellulose is basically the same. Although the ability to degrade cellulose is weakened at 30°C, the decline is not large. The KY240 strain also has the ability to decompose potassium and inorganic phosphorus, and is expected to be used to prepare bacterial fertilizer for degrading cellulose, promote straw maturity, improve soil fertility, especially increase the amount of potassium and phosphorus available to plants in the soil.

Description

technical field [0001] The invention relates to a Pseudomonas graminis strain capable of degrading cellulose at low temperature and its application. Background technique [0002] Lignocellulose is the most abundant and cheapest renewable resource on earth. Lignocellulose includes three major classes of polymers: cellulose, hemicellulose, and lignin. Cellulose fibrils are closely intertwined with lignin and hemicellulose through covalent and non-covalent bonds in the vascular tissue of higher plant cell walls to form lignocellulose. The amount and type of lignocellulose varies among species, ages and parts of the same plant. Generally speaking, lignocellulose contains 39% cellulose, 30% hemicellulose, and 25% lignin [1] . Cellulose is a linear polymer composed of tens of thousands of glucose molecules with cellobiose (glucose-β-1,4-glucose) as the basic repeating unit. The β-1,4-linked glucose residues in the cellulose chain rotate 180° relative to the adjacent units, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C12R1/38
CPCC12N1/20C05F11/08C05G3/00C12R2001/38C12N1/205Y02W30/40C12N2523/00
Inventor 屈庆涛黄燕菲梁韵玲苑莹康耀卫
Owner BEIJING ZNFY GRP CO LTD
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