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Preparation method of protein nanoparticles

A nanoparticle and protein technology, applied in the field of biomedicine, can solve the problem of protein activity reduction and achieve the effect of maintaining activity

Active Publication Date: 2020-04-21
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, modification by covalent bonds in the prior art involves chemical reactions, which easily leads to the reduction of protein activity.

Method used

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  • Preparation method of protein nanoparticles
  • Preparation method of protein nanoparticles
  • Preparation method of protein nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] According to the method of the present invention, 1 mg of TRAIL protein was weighed and dissolved in 1 mL of phosphate buffer solution; 1 mg of diphenylalanine was weighed and dissolved in 8 μL of hexafluoroisopropanol. The above two solutions were quickly mixed and allowed to stand for 10 minutes; centrifuged for 10 minutes at a speed of 10,000 rpm. The supernatant was sucked off, and the nanoparticle pellet was washed 3 times.

Embodiment 2

[0061] According to the method of the present invention, 2 mg of TRAIL protein was weighed and dissolved in 1 mL of phosphate buffer solution; 1 mg of diphenylalanine was weighed and dissolved in 8 μL of hexafluoroisopropanol. The above two solutions were quickly mixed and allowed to stand for 10 minutes; centrifuged for 10 minutes at a speed of 10,000 rpm. The supernatant was sucked off, and the nanoparticle pellet was washed 3 times.

Embodiment 3

[0063] According to the method of the present invention, 5 mg of TRAIL protein was weighed and dissolved in 1 mL of phosphate buffer solution; 1 mg of diphenylalanine was weighed and dissolved in 8 μL of hexafluoroisopropanol. The above two solutions were quickly mixed and allowed to stand for 10 minutes; centrifuged for 10 minutes at a speed of 10,000 rpm. The supernatant was sucked off, and the nanoparticle pellet was washed 3 times.

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Abstract

The invention relates to a preparation method of protein nanoparticles. The protein nanoparticle is obtained by assembling a protein or a polypeptide and a small molecule peptide through an intermolecular force. The small molecule peptide is composed of one or three of phenylalanine, tryptophan, lysine, aspartic acid, glutamic acid, histidine and tyrosine. The protein comprises hemoglobin, bovineserum albumin and a tumor necrosis factor superfamily. The preparation method comprises the following steps: mixing a protein or a polypeptide and a small molecule peptide, and standing and separatingthe mixture to obtain the protein nanoparticles. The method for preparing the protein nanoparticles has the advantages of quickness, simplicity, high efficiency, capability of maintaining protein activity to a great extent and the like, and provides a new scheme for preparation of protein nano-materials.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of protein nanoparticles with good biocompatibility, in particular to a preparation and synthesis method of a nanocomposite material assembled by intermolecular forces. Background technique [0002] Supramolecular self-assembly refers to the process in which polymers spontaneously form aggregates with special structures or shapes under the impetus of non-covalent forces such as hydrophobic forces, van der Waals forces, and hydrogen bonds. Different from traditional molecular chemistry, supramolecular assembly chemistry provides a platform for researchers to prepare highly complex chemical systems. It is an important scientific frontier that integrates chemistry, physics, molecular biology and nanoscience, and is also a fundamental research area. areas in need of development. As a class of advanced dynamic, non-covalent assembly materials, bioactive sup...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K38/19A61K38/38A61K38/42A61K38/44A61K38/48A61K47/18A61K47/42A61P35/00
CPCA61K9/1617A61K9/1658A61K38/191A61K38/385A61K38/42A61K38/443A61K38/4826A61K38/488A61P35/00C12Y111/01006C12Y304/21004C12Y304/23
Inventor 李峻柏崔巍赵洁李伟
Owner INST OF CHEM CHINESE ACAD OF SCI
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