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Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve problems affecting tomato quality, consumer health, etc.

Active Publication Date: 2020-04-21
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the prolongation of the use of the agent, tomato early blight bacteria not only become resistant to it, but also a large number of tomatoes are ready-to-eat fruits and vegetables, and the pesticide residues have seriously affected the quality of tomatoes and the health of consumers.

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The isolation and screening of embodiment 1 bacterial strain

[0022] 1. Isolation and purification of bacteria: Collect healthy plant leaves and rhizosphere soil from rapeseed fields and wheat fields, weigh 10g each of the plant leaf and rhizosphere soil samples, and crush the leaf tissues with a sterile mortar. Put the tissue and rhizosphere soil into a triangular flask filled with 50mL sterile water, 180r·min -1 Shake for 10 min to obtain tissue suspension and soil suspension. The suspension was serially diluted for 10 by the dilution separation method. -1 ~10 -6 , 100 μL of each gradient was evenly spread on nutrient agar (NA) plates, each concentration was replicated three times, and then placed in a 28°C constant temperature incubator for upside-down culture. After bacterial colonies grow out, pick representative single colonies with different morphological characteristics, streak and purify them on NA plates, store the purified bacterial strains on the slant o...

Embodiment 2

[0033] Antibacterial research of embodiment 2 antagonistic strains

[0034] Inhibitory effect of antagonistic bacteria sterile filtrate on pathogenic fungus mycelial growth: The mycelial growth rate method was used to determine the inhibitory effect of strain T23 on Sclerotinia sclerotiorum, tomato early blight and Trichosanthes anthracnose. The antagonistic strain T23 was inoculated on the NA plate and cultured at 28°C for 24h. Pick a single colony and inoculate it in a Erlenmeyer flask filled with 100mL NA liquid medium, at 28°C, 180r min -1 Under the condition of shaking culture for 24 hours, the fermentation broth of strain T23 was obtained. The fermentation broth of the strain was heated at 4°C, 12000r min -1 After centrifugation for 20 min, the supernatant obtained after centrifugation was filtered through a bacterial filter with a pore size of 0.22 μm to obtain a sterile filtrate of the antagonistic strain T23. Mix the obtained sterile filtrate with the PDA liquid me...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to bacillus amyloliquefaciens and an application thereof. According to the invention, one strain T23 with strong antagonism to various pathogenic bacteria is separated and screened from various plant tissues and rhizosphere soil thereof by adopting dilution separation and plate confrontation culture methods. Throughmorphological and physiological and biochemical characteristic tests and 16s rRNA and gyrB gene sequence analysis, the bacterium is identified as bacillus amyloliquefaciens. The activity of metabolites of the strain is further determined, and the prevention and treatment effect of the antagonistic strain on tomato early blight, sclerotinia rot of colza and snakegourd fruit anthracnose is determined through a greenhouse pot experiment, so that the subsequent research and development of biocontrol agents for tomato early blight, sclerotinia rot of colza and snakegourd fruit anthracnose are convenient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of bacillus amyloliquefaciens and its application. Background technique [0002] The occurrence of plant diseases seriously affects the yield of crops and the quality of their products. Because the cost of agricultural control is relatively high, chemical control is easy to cause pollution to the environment, and the cycle of cultivating disease-resistant varieties is long, biological control is considered to be a promising way of control in recent years. As a potential new disease control method, biological control is to use the interaction between biological species to inhibit another or another type of organisms by one or another type of organisms, so as to prevent and control diseases. Because this control method will not cause irreversible pollution to the environment, has high safety, and will not produce directional selection to make the disease resistant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 张立新汪霞李相汉林彬檀根甲
Owner ANHUI AGRICULTURAL UNIVERSITY
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