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SNP molecular marker for distinguishing Takifugu fasciatus Zhongyang No. 1 from common Takifugu fasciatus and application of SNP molecular marker

A technique of puffer obscurus and molecular markers, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of CIRP combined with SNP site population identification and molecular assisted breeding applications, etc. To achieve the effect of convenient operation

Active Publication Date: 2020-04-24
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of CIRP combined with SNP loci for population identification and molecular assisted breeding

Method used

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  • SNP molecular marker for distinguishing Takifugu fasciatus Zhongyang No. 1 from common Takifugu fasciatus and application of SNP molecular marker
  • SNP molecular marker for distinguishing Takifugu fasciatus Zhongyang No. 1 from common Takifugu fasciatus and application of SNP molecular marker
  • SNP molecular marker for distinguishing Takifugu fasciatus Zhongyang No. 1 from common Takifugu fasciatus and application of SNP molecular marker

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Experimental program
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Effect test

Embodiment 1

[0036] a) Obtaining of puffer puffer populations:

[0037]The present invention carries out the low-temperature-resistant experimental treatment on the new species of puffer puffer obscura "Zhongyang No. 1" cultivated in the Lukou Experimental Base of the Nanjing Fishery Science Research Institute. Taking the out-of-balance time as the low-temperature resistance parameter, and then performing SPSS correlation analysis on the out-of-balance time and the genotype of the SNP locus to obtain the low-temperature-resistant dominant genotype. The specific experimental process is as follows: after the film was released in late April 2019, it was cultured in the same environment (temperature, density, etc.) in the pool (580) for 191 days, and 121 tails were selected with a length of about 15 cm ± 0.5 cm. For fish with good vigor, stop feeding for 1 day before the experiment; cool down at a rate of 1°C / h from room temperature of 25°C, and cool down at a rate of 5°C / h when the temperatur...

Embodiment 2

[0064] a) Obtaining of different puffer puffer populations:

[0065] The fish used in the experiment of the present invention were taken from four groups: in September 2018, 107 pufferfish species "Zhongyang No. 1" about 5 months old were taken from Jiangsu Zhongyang Group Co., Ltd. (Hai'an); Collected from 99 common unselected Fugu obscurus populations about 4 months old in Zhenjiang Jiangzhiyuan Fishery Technology Co., Ltd. (Yangzhong) in October; in October 2018 from Jiangyin Shengang Sanxian Breeding Co., Ltd. (Jiangyin) in May There were 96 unselected puffer puffer populations around the same age; 121 fugu obscurus, a new breed of pufferfish species "Zhongyang No. 1", were bred in the Lukou Experimental Base of Nanjing Fishery Science Research Institute (Nanjing). Caudal fins were stored in 95% ethanol at -20°C for genomic DNA extraction.

[0066] b) Genomic DNA extraction of different puffer puffer populations;

[0067] The method for extracting genomic DNA from differ...

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Abstract

The invention discloses an SNP molecular marker for distinguishing Takifugu fasciatus Zhongyang No. 1 from common Takifugu fasciatus and application of the SNP molecular marker. The sequence of the SNP molecular marker is shown as SEQ ID NO: 1 (derived from a CIRP gene sequence of Takifugu fasciatus); and the SNP molecular marker is located at the 66th site, namely an SNP site, of the sequence shown as SEQ ID NO: 1, and the basic group of the SNP site is A or T. The SNP site disclosed by the invention is remarkably related to a low temperature resistance character, and can be used for distinguishing a new Takifugu fasciatus variety Zhongyang No. 1 group from a common Takifugu fasciatus group. The SNP site can also be effectively applied to early identification fry of a new Takifugu fasciatus variety Zhongyang No. 1 and common Takifugu fasciatus. A method provided by the invention is accurate in operation, and is simple and practical.

Description

technical field [0001] The invention relates to a SNP molecular marker for distinguishing "Zhongyang No. 1" from common puffer puffers and its application, and belongs to the field of puffer puffer genetics and breeding. Background technique [0002] Takifugu fasciatus, commonly known as puffer, belongs to Osteichthyes, Actinopterygii, Letrodontiforms, Tetraodontidae, Takifugu, Domestically, it is mainly distributed in China's Yellow Sea, Bohai Sea and offshore waters, rivers, seas and lakes. Puffer obscurus is a kind of aquaculture fish with high economic, nutritional and breeding value, which is very tender and plump, and has high nutritional value. It is deeply loved by consumers. Fugu obscurus is one of the few species in the genus Fugu that has the habit of migrating into sea and fresh water. Every October, when the temperature drops to about 18°C, it cannot survive normally in the middle and lower reaches of the Yangtze River. It needs to go to the sea to survive the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 尹绍武徐杰杰王涛程景颢毕宜慧张鑫宇李杰
Owner NANJING NORMAL UNIVERSITY
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