Bipolaris sorokiniana loop mediated isothermal amplification (LAMP) detection primer group and application thereof

A technology for wheat root rot fungus and Helminthospora soxhlet, which is applied in the field of genetic engineering and can solve problems such as threats to safe production of wheat

Pending Publication Date: 2020-05-01
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

In recent years, with the implementation of straw returning technology, the occurrence of whe

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  • Bipolaris sorokiniana loop mediated isothermal amplification (LAMP) detection primer group and application thereof
  • Bipolaris sorokiniana loop mediated isothermal amplification (LAMP) detection primer group and application thereof
  • Bipolaris sorokiniana loop mediated isothermal amplification (LAMP) detection primer group and application thereof

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention.

[0019] 1 Materials and methods

[0020] 1.1 Tested strains

[0021] A total of 14 kinds of plant pathogenic bacteria were tested, all of which were important pathogens of wheat and common soil-borne pathogens, including 8 strains of wheat root rot fungus Helminoptera soxleyi and 1 strain of other pathogenic bacteria. The specific information is shown in Table 1.

[0022] surface Information on strains used for LAMP detection

[0023]

[0024] 1.2 Test medium

[0025] The test media were potato dextrose agar (PDA) and V8 juice agar (V8A). PDA medium: potato 200 g, glucose 20 g, agar powder 15 g, distilled water 1L; V8A medium: V8 juice 200 mL, calcium carbonate 3 g, agar powder 15 g, distilled water 800 mL; The medium is the corresponding liquid medium without adding agar powder.

[00...

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Abstract

The invention relates to a bipolaris sorokiniana loop mediated isothermal amplification (LAMP) detection primer group and application thereof, and belongs to the technical field of genetic engineering. According to the bipolaris sorokiniana LAMP detection primer group, an LAMP detection technology of bipolaris sorokiniana host is established by taking ITS of the pathogenic bacterium, namely the bipolaris sorokiniana, as a target sequence; and the adopted primers are high in specificity, and high in reaction system sensitivity. Thus, the reaction system is capable of accurately and rapidly detecting the bipolaris sorokiniana from diseased wheat tissue. The detection method of the bipolaris sorokiniana is simple, rapid and sensitive, and requires no expensive equipment; and thus, the detection method is conducive to large-scale promotion and use in grass-root plant protection stations so as to provide technical support for early and precise control of the bipolaris sorokiniana.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a primer set for detecting wheat root rot fungus Helminthosporium soxleyi LAMP and an application thereof. Background technique [0002] Wheat root rot is an important disease in wheat production, which occurs in various wheat regions in my country. The disease can occur from the seedling stage to the adult plant stage, and mainly infects the stem base and root system of wheat, causing stem base rot and root rot, and in severe cases, the whole plant dies. In recent years, with the implementation of straw returning technology, the occurrence of wheat root rot has shown an upward trend, which has posed a certain threat to the safe production of wheat. At present, root rot of wheat in my country is mainly caused by Helminthosporium soxleyi Bipolaris sorokinian a and Fusarium Fusarium spp. and other fungal infections, and the early symptoms of wheat root ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6844C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895C12Q1/6844C12Q2531/119
Inventor 胡艳红崔林开和志华李梦琪邓俊丽
Owner HENAN UNIV OF SCI & TECH
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