Method for analyzing linear epitopes of vegetable food allergens based on bioinformatics

A bioinformatics, food allergy technology, applied in bioinformatics, informatics, sequence analysis, etc., can solve problems such as high blindness, heavy workload, and difficulty in ensuring accuracy

Pending Publication Date: 2020-05-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The peptide synthesis technology is to incubate the synthesized short overlapping peptides with the serum of allergic patients through dot blotting or immunoblotting to detect their binding to specific IgE antibodies, thereby judging and screening linear epitopes, but it is necessary to collect serum from allergic patients , time-consuming, heavy workload, high blindness, it is possible to ignore epitopes in overlapping intervals, and it is difficult to guarantee accuracy

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  • Method for analyzing linear epitopes of vegetable food allergens based on bioinformatics
  • Method for analyzing linear epitopes of vegetable food allergens based on bioinformatics
  • Method for analyzing linear epitopes of vegetable food allergens based on bioinformatics

Examples

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Embodiment 1

[0042] The peanut allergen Ara h1 is a 7S globulin and belongs to the Cupin superfamily. Serological analysis found that it can be recognized by more than 90% of peanut allergic patients. Because the structural sequence of the Cupin superfamily has the characteristics of high conservation, strong thermal stability, resistance to enzymatic hydrolysis and indigestibility, Ara h1 was selected as the model allergen in the present invention.

[0043] This embodiment provides a method for analyzing the linear epitope of peanut allergen Ara h1 based on bioinformatics, including the following steps:

[0044] (1) Obtain the complete amino acid sequence of Ara h 1 from the Uniprot database; obtain the 23 linear epitope sequences of Ara h 1 from the allergenic protein structure database SDAP, as shown in Table 1.

[0045] Table 1 Linear epitope sequence of peanut major allergen Ara h 1

[0046]

[0047]

[0048] Note: The key amino acid residues of the IgE binding polypeptide are underlined.

[0...

Embodiment 2

[0057] The walnut allergen Jug r 2 belongs to the 7S pea globulin family, which is the same family as the peanut allergen Ara h1. The primary sequence is composed of 593 amino acids and the molecular weight is about 44kDa.

[0058] This embodiment provides a method for analyzing the linear epitope of walnut allergen Jug r 2 based on bioinformatics, which includes the following steps:

[0059] (1) Homologous modeling prediction of Jug r 2 tertiary structure

[0060] The BLAST search in the PDB database has high homology with the allergen Jug r 2, and the protein sequence of the tertiary structure data has been analyzed by experiments. The results are as follows Image 6 As shown, the 7S pea globulin with PDB access number 5e1r.1.A has the highest matching degree in terms of sequence similarity and coverage, and was selected as the best template for Jug r 2 modeling. 5e1r.1.A is the crystal structure of the 7S pea globulin food allergen of hickory nut. It has a total length of 426 amin...

Embodiment 3

[0071] Wheat allergen CM16 is a protein with a molecular weight of 17kDa and 143 amino acids. It belongs to the gliadin superfamily and contains multiple cysteine ​​residues, which can form intramolecular disulfide bonds to ensure the structural stability of the protein. Has heat resistance.

[0072] This embodiment provides a method for analyzing the linear epitope of wheat allergen CM16 based on bioinformatics, including the following steps:

[0073] (1) Prediction of CM16 linear epitope

[0074] Obtain the amino acid sequence of wheat allergen CM16 from the Uniprot database, use the Hoop-woods amino acid hydrophilicity analysis program in the bioinformatics software DNAStar Protean, the solvent accessibility algorithm of Emini-Surface Probability, the plasticity analysis of Kparlus-Schuzl and Jameson-Wolf's antigen index analysis program analyzes possible linear epitopes of CM16.

[0075] The result is Picture 11 As shown, in the amino acid hydrophilicity analysis, the hydrophili...

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Abstract

The invention provides a method for analyzing the linear epitopes of vegetable food allergens based on bioinformatics. The method comprises the following steps: acquiring the linear epitopes, amino acid sequences, secondary structures and tertiary structures of vegetable food allergens by utilizing a bioinformatic database; and analyzing the linear epitopes by utilizing bioinformatic software, wherein the amino acid composition and occurrence frequency of the linear epitopes, the positions of the linear epitopes in the secondary structures and the positions of the linear epitopes in the tertiary structures are analyzed. The amino acid sequences, the secondary structures, the tertiary structures and the like of the vegetable food allergens are analyzed by utilizing the bioinformatic database and software, and therefore, the positional relations and characteristics of the linear epitopes of the vegetable food allergens and the advanced structures of the linear epitopes of the vegetable food allergens are analyzed; and the method is rapid and accurate, no experiment is needed, and deep research on the linear epitopes is beneficial for research on food allergenicity.

Description

Technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for analyzing linear epitopes of plant food allergens based on bioinformatics. Background technique [0002] Food allergy is an adverse reaction that occurs when the body is exposed to a specific food, that is, an allergic reaction that can involve different organs of the body caused by the immune system's specific immune response to allergens. According to epidemiological surveys, the incidence of infants and young children worldwide has reached 8%, and adults have reached 5%. Food allergies are mostly type I hypersensitivity reactions mediated by IgE, which can cause systemic multi-system reactions such as asthma, diarrhea, urticaria, allergic dermatitis, and even shock and life-threatening, seriously affecting the quality of life of patients. So far, the most effective way to prevent food allergies is to avoid contact with food allergens. However, because t...

Claims

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Application Information

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IPC IPC(8): G16B50/30G16B30/10
Inventor车会莲杨帅李欣芮陈成范卓妍张亚妮贺晓云
OwnerCHINA AGRI UNIV