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Tissue culture and rapid propagation method for Ilex crenata Thunb by taking embryos as explants

A technology of holly serrata, tissue culture and rapid propagation, applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of restricted promotion and reproduction, low germination rate, low transplant survival rate

Inactive Publication Date: 2020-05-12
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low germination rate of Ilex serrata tissue culture seedlings in the current tissue culture method, the transplanting survival rate is low, which limits its large-scale promotion and propagation.

Method used

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  • Tissue culture and rapid propagation method for Ilex crenata Thunb by taking embryos as explants
  • Tissue culture and rapid propagation method for Ilex crenata Thunb by taking embryos as explants
  • Tissue culture and rapid propagation method for Ilex crenata Thunb by taking embryos as explants

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for tissue culture and rapid propagation of Ilex serrata using embryos as explants provided in this embodiment includes:

[0021] (1) in August, when the fruit is green and the size reaches the maximum, the fruit is collected for embryo peeling;

[0022] (2) Rinse the immature seeds of Ilex serrata with running water for 20 minutes to remove surface dirt. Transfer to an ultra-clean workbench, put into a sterile beaker, and rinse once with sterile water. The surface disinfection of seeds is first soaked in 75% alcohol for 5 minutes, then soaked in 8% sodium hypochlorite for 15 minutes, and finally rinsed with sterile water for 5-6 times, and put into sterile water for later use;

[0023] (3) The immature embryos are peeled off, inoculated on the WPM basic medium, and cultivated in the dark for 12 to 16 days;

[0024] (4) When the young embryos germinate, they are transferred to light conditions and cultured for 26 to 30 days;

[0025] (5) Wash the seedlings a...

Embodiment 2

[0027] The basic medium used in this embodiment is 1 / 4MS medium, and the rest of the steps are the same as in Example 1.

Embodiment 3

[0029] The basic medium used in this embodiment is 1 / 2 MS medium, and the remaining steps are the same as in Example 1.

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Abstract

The invention discloses a tissue culture and rapid propagation method for Ilex crenata Thunb by taking embryos as explants, which comprises the following steps: step 1, collecting Ilex crenata Thunb fruits, and cleaning and disinfection of the Ilex crenata Thunb fruits; step 2, peeling off to obtain young embryos, inoculating the young embryos on a basic culture medium, and carrying out dark culture for 12-16 days; 3, after the young embryo germinates, transferring the young embryo to an illumination condition to be cultured for 26-30 days; step 4, cleaning the seedlings and transplanting theseedlings into a culture medium for culture; Ilex crenata Thunb plant tissue rapid propagation system is established by taking immature zygotic embryos of Ilex crenata Thunb as explants, the germination time of Ilex crenata Thunb is shortened to 5-7 weeks from 2-3 years of conventional breeding, the germination rate of Ilex crenata Thunb tissue culture seedlings is up to 100%, and the transplanting survival rate is up to about 90%.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture and rapid propagation of Ilex serrata using embryos as explants. Background technique [0002] Ilex serrata is a multi-branched evergreen shrub, up to 5 meters high; the leaves are leathery, obovate, elliptic or oblong-elliptic, 1-7 male flowers are arranged in cymes, 4 bases, white; calyx disc-shaped , petals 4, broadly elliptic, corolla diameter about 6 mm, petals ovate, ovary ovoid, fruit spherical, black when mature; endocarp leathery. The flowering period is from May to June, and the fruiting period is from August to October. Born in hills, mountain forests or bushes at an altitude of 700-2100 meters. Distributed in China, Japan and Korea. It is often cultivated as an ornamental tree species in gardens, and it is also cultivated in Europe and America. The fruit of Ilex serrata belongs to the seeds of plants, and it is very difficult to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 杨玉洁郝明灼邹义萍耿芳
Owner YANGTZE UNIVERSITY
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