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Centrifugal micro-fluidic chip and sample processing method

A microfluidic chip and centrifugal technology, applied in the direction of chemical instruments and methods, instruments, laboratory utensils, etc., can solve problems such as inappropriate structures, and achieve the effects of saving time, avoiding sample pretreatment process, and convenient operation

Pending Publication Date: 2020-05-12
苏州含光微纳科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The chip converts unmeasured samples into multiple aliquots of accurate samples through capillary action and detection of centrifugal force when the chip rotates, which can realize multi-indicator detection, but the above structure is not suitable when the samples need to be pretreated

Method used

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  • Centrifugal micro-fluidic chip and sample processing method
  • Centrifugal micro-fluidic chip and sample processing method
  • Centrifugal micro-fluidic chip and sample processing method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] figure 1 It is a structural schematic diagram of the centrifugal microfluidic chip 100 of this embodiment. The centrifugal microfluidic chip 100 includes a liquid inlet hole 101, an aliquot hole 102, a first siphon pipe 103, a sample pretreatment area 104, an exhaust hole 105, a second siphon pipe 106, reaction holes 107, 108, and a waste liquid pool 109 . figure 1 There are two groups of 101-109, and these two groups of 101-109 are connected through 102.

[0031] The centrifugal microfluidic chip 100 above is installed on a centrifuge to form a centrifugal microfluidic system to process samples, including the following steps:

[0032] 1) Add a certain amount of sample into the liquid inlet hole 101 .

[0033] 2) Start the centrifuge, the rotating speed is in the range of 100-3000rpm / s, and the time is in the range of 20-40s. Since the aliquot hole 102 includes two equally divided structures, that is, two chambers with equal volumes, and the liquid level after centr...

Embodiment 2

[0042] figure 1 In the structure shown in Example 1, there are two groups 101-109 on the centrifugal microfluidic chip 100. In this embodiment, more groups 101-109 are set up and evenly distributed on the centrifugal microfluidic chip 100, such as figure 2 The structure shown, thereby improving the processing efficiency of the sample.

[0043] Other structures of the centrifugal microfluidic chip in this embodiment are the same as those in Embodiment 1.

Embodiment 3

[0045] figure 1 In the structure shown in Example 1, there are two reaction holes, namely 107 and 108 . In this embodiment, one or more than two reaction wells are provided.

[0046] Other structures of the centrifugal microfluidic chip in this embodiment are the same as those in Embodiment 1.

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Abstract

The invention relates to a centrifugal micro-fluidic chip and a sample processing method. The centrifugal micro-fluidic chip comprises a liquid inlet hole, an equal division hole, a first siphon pipeline, a sample pretreatment area, a second siphon pipeline, reaction holes and a waste liquid pool which are connected in sequence, wherein the sample pretreatment area is also connected with an exhaust hole, the equal division hole comprises two cavities with equal volumes and is used for realizing equal division of samples, the volumes of the reaction holes are equal so as to achieve the secondary equal division of the samples, and the sample pretreatment area and each reaction hole are loaded with different reaction reagents as required to realize various detection applications, or loaded with the same reaction reagent to realize parallel detection of the same sample. According to the invention, sample pretreatment and multiple equal division treatment can be realized, the operation is convenient, and the time is saved.

Description

technical field [0001] The invention belongs to the technical field of medical consumables, and in particular relates to a centrifugal microfluidic chip and a sample processing method using the centrifugal microfluidic chip, which can realize sample pretreatment and multiple aliquot processing. Background technique [0002] Centrifugal microfluidic chips are medical consumables, used in the field of instant diagnosis, and can output reaction results conveniently and quickly. It is characterized in that the microfluidic structure is integrated on a disc-shaped chip, and the flow of the microfluid is driven by centrifugal force, so as to realize the detection and analysis of samples. The centrifuge system can complete sample pretreatment, mixing, separation and detection of precise volume, etc. In recent years, the centrifugal microfluidic system has achieved rapid development due to its advantages of high throughput, high integration, multiple parallel analysis, portability,...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N35/00G01N35/10
CPCB01L3/50273G01N35/00G01N35/10G01N2035/00237G01N2035/00495G01N2035/1032G01N2035/1034
Inventor 宋璐孙丽亚安光明吴烨娴陈兢
Owner 苏州含光微纳科技有限公司
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