Full-automatic nucleic acid extraction, amplification and detection instrument

A detector, fully automatic technology, applied in the direction of biochemical instruments, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of manpower consumption, insufficient automation, and errors in detection results, so as to improve convenience and convenience The effect of adjusting, improving convenience and flexibility

Pending Publication Date: 2020-05-15
广州市宝创生物技术有限公司
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  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of insufficient automation, high cost, labor-intensive, and low efficiency of detection results in the nucleic acid extraction and amplification detection equipment in the prior art, the present invention provides a method that integrates the functions of nucleic acid extraction, amplif

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  • Full-automatic nucleic acid extraction, amplification and detection instrument
  • Full-automatic nucleic acid extraction, amplification and detection instrument
  • Full-automatic nucleic acid extraction, amplification and detection instrument

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Example Embodiment

[0085] Example 1: Reference Figure 14 , Figure 13 It is a schematic cross-sectional view of the chassis 212 in the first embodiment of the present invention, regarding the experimental steps of the automatic instrument in the present invention to extract, amplify, and detect PCR:

[0086] Among them, the equipment experiment process: the temperature of the sample tank 21211 is raised to 70°C through the thermal cycler 23 of the present invention;

[0087] 1). Prepare a 100ul sample, open the front door 3 of the working box 1, open the sample cover 2112, add 100ul sample to the sample tank 21211 through the sample hole 2111, and mix;

[0088] 2). Select the test items required by the sample on the operation display 4, press the start button on the operation display 4, and realize the reset of the nucleic acid extraction and amplification detection device 21 through the power device 22, and start a whole The work of automatic nucleic acid extraction and amplification detector;

[0089...

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Abstract

The invention discloses a full-automatic nucleic acid extraction, amplification and detection instrument, which comprises a working box body and an operation display screen, wherein a machine core device is arranged in the working box body, is electrically connected with the operation display screen and comprises a nucleic acid extraction, amplification and detection device, a thermal cycling device, an adjustable light supplement lamp device and an optical detection device; and the optical detection device is installed in the machine core device and is positioned above the nucleic acid extraction, amplification and detection device. A start button on the operation display screen is pressed down, and the nucleic acid extraction, amplification and detection device carries out nucleic acid extraction, amplification and detection; after detection is finished, the adjustable light supplement lamp device can be automatically on so as to assist the optical detection device in photographing and detecting the nucleic acid extraction, amplification and detection device; through the operation display screen, a sample detection situation can be displayed; a whole process can be automaticallycarried out and does not need people to intervene and participate, an automation degree is high, errors and cross contamination caused by manual detection can be avoided, and efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, in particular to a fully automatic nucleic acid extraction and amplification detection. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides. It is one of the most basic substances of life and widely exists in all animal and plant cells and microorganisms. The carrier of information, the most important biological information molecule, is the main object of molecular biology research. Therefore, the extraction of nucleic acid is the most important and basic operation in molecular biology experimental technology, which is of great significance to epidemic prevention and control and epidemiological investigation. It has good application prospects and is also very suitable for biological anti-terrorism, agriculture and food safety, biomedicine and other fields. [0003] The early experiments were done by manual extraction and am...

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/00
CPCC12N15/1003C12Q1/6806
Inventor 杨祥胜邱小龙肖慧张淞
Owner 广州市宝创生物技术有限公司
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