Composition and application thereof, and sperm refrigerating fluid and preparation method thereof

Abstract

The invention relates to the field of assisted reproductive technology, and discloses a composition and application thereof, and a sperm refrigerating fluid and a preparation method thereof. The spermrefrigerating fluid comprises a basic culture solution, the composition, a cryoprotectant, human serum albumin and a bactericide, wherein the composition is prepared from resveratrol with a concentration of 12 to 26mg / L, reduced glutathione with a concentration of 0.2 to 1.9 mg / L , lecithin with a concentration of 436 to 775 mg / L, cholesterol with a concentration of 0.5 to 1.6 mg / L, an ATP sodiumsalt with a concentration of 9 to 17 mg / L, heparin with a concentration of 11 to 28 mg / L, phosphatidylserine with a concentration of 93 to 299 mg / L and coenzyme Q10with a concentration of 5 to 25 mg / L; and the cryoprotectant comprises paclitaxel with a concentration of 0.3 to 1.1 mg / L of, trehalose with a concentration of 16 to 22 g / L, cane sugar with a concentration of 7 to 13 g / L and glycerol with a concentration of 80 to 250 mL / L. The sperm refrigerating fluid can reduce damage of sperms in low-temperature refrigeration and in freezing recovery, improve vitality of the sperms after freezing recovery, prolong the survival time of the sperms after freezing recovery, promote sperm capacitation and enhance penetrating fertilization capacity of the sperms to egg cells; and thus, the fertilization success rate and blastocyst formation rate of the ova are improved, and finally, the effect of improving the pregnancy success rate of an in-vitro fertilization assisted reproduction technologyis achieved.

Description

technical field [0001] The invention relates to the technical field of assisted reproduction, in particular to a composition and its application, a sperm freezing liquid and its preparation method. Background technique [0002] In vitro fertilization (in vitro fertilization, IVF), also known as test-tube baby, is a method of assisted reproductive therapy, specifically refers to the fertilization of sperm and eggs under specific environmental conditions outside the body after the eggs and sperm are taken out of the body respectively. Fertilized eggs are formed, and the fertilized eggs are continuously cultured and developed in vitro to embryos at the blastocyst stage, and then the embryos are transferred back to the mother's uterus for pregnancy and development into fetuses. [0003] In the assisted reproductive technology of in vitro fertilization, men can freeze their own sperm in medical institutions with corresponding qualifications based on the consideration of future eu...

AI Technical Summary

Problems solved by technology

[0004] At present, the existing sperm freezing solutions on the market are mainly divided into two categories, namely those containing egg yolk and those without egg yolk, but both of them have some disadvantages: on the one hand, since the egg yolk contains not only lecithin, but also many complex Components. Granular substances will appear after the sperm cryopreservation with yolk-containing sperm freezing liquid, which will affect the further processing process. At the same time, because the yolk is a heterologous substance, there is still a certain biological risk; on the other hand, the sperm without yolk The cryoprotective effect on sperm is limited because the cryogenic solution only contains basic salt ions, a small amount of amino acids, and a single sperm cryoprotectant, and there is a lot of room for improvement

Images