Application of bpvnd1 gene

A technology of gene and gene expression, applied in the field of birch cultivation

Active Publication Date: 2021-10-01
FORESTRY RES INST OF HEILONGJIANG PROVINCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing birch forest resources are still unable to meet the huge demand of the market. High-quality birch seedlings are the basis of birch breeding, and shortening the growth cycle of birch as much as possible is still one of the important topics currently researched by Chinese birch breeders.

Method used

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  • Application of bpvnd1 gene
  • Application of bpvnd1 gene
  • Application of bpvnd1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Production of birch transgenic plants:

[0037] Vector construction: select a 256bp sequence (SEQ ID NO.3) in the coding region of the BpVND1 gene:

[0038] GTTCAGCTTCCTCAACTAGAGAGCCCATCTCTGCCGTTAATAAAGAGGCCGCCAAGCTCAATATCTCTGATATCAGAAAATAATGAAGAAGAAGAGCAAATTATGAATAGAGGGTGCAAACAACCCAGAAAGTGACTGATTGGAGGGCCCTTGACAAGTTTGTTGCTTCTCAATTGAGTCAAGAAGATAGATTTGAGGGTGATGGAGAATCAAGCTTTGGCGACCACACAGGG.

[0039] Respectively introduce restriction sites to design primers to amplify the forward and reverse sequences, and then insert these two sequences into the pFGC5941 vector respectively to construct the RNAi vector pFGC5941-ProBpVND1 of the BpVND1 gene.

[0040] Table 1 Primers used in the construction of pFGC5941-ProBpVND1 vector

[0041]

[0042] Birch transgene: the constructed RNAi vector was transferred into the birch genome by Agrobacterium-mediated leaf disc transformation. The specific method is as follows: activate the Agrobacterium strain of the RNAi carrier, shake the...

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Abstract

The invention relates to the application of BpVND1 gene and belongs to the technical field of birch cultivation. The present invention provides the application of BpVND1 gene in increasing the growth of birch. The nucleotide sequence of the BpVND1 gene is shown in SEQ ID NO.1. The application of the BpVND1 gene of the present invention can increase the growth and biomass of white birch compared with wild type white birch, increase the cellulose content and fiber length, and reduce the lignin content, which is suitable for the cultivation of pulp wood and fuel wood.

Description

technical field [0001] The invention relates to the technical field of birch cultivation, in particular to the application of the BpVND1 gene. Background technique [0002] Wood is an indispensable and important industrial raw material in the construction and development of the national economy. With the rapid development of economy and society, the use of wood has changed rapidly, and the requirements for both its amount and its material have been continuously improved. Therefore, increasing timber production and improving timber quality are the long-term responsibilities of forestry workers. During the vigorous development of forestry, the older generation of foresters have made outstanding contributions to forest tree improvement by using conventional breeding methods, and have gained attention. However, using conventional provenance selection and hybrid breeding methods, the breeding cycle is long, the process is complicated, it is greatly affected by the outside world, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/00
CPCC07K14/415C12N15/8205C12N15/8261
Inventor 李红艳王丙锋马盈王艳敏温爱亭谷维苏雅迪罗旭罗玉亮赵越兰士波张玲张海峰
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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