Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compound Disaspidin BB and application of compound Disaspidin BB in antibiosis

A compound and antibacterial technology, applied in the field of medicine, can solve the problem of very few active ingredients research, and achieve the effect of promoting effective application, clear structure and good application prospect.

Pending Publication Date: 2020-06-12
GUANGDONG PHARMA UNIV +1
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dryopteris fragrans (L.) Schott belongs to the genus Dryopteris fragrans (L.) Schott, and is widely distributed in many countries. In China, it is mainly distributed in Wudalianchi Geological Park of Heilongjiang Province, Baikashou Mountain in Tahe County, Huzhong In the alpine area of ​​the Dabai Mountain and the northern part of the Xiaoxing’an Mountains, it grows in forests at an altitude of 700-2400 meters or on talc slopes in alpine regions, on gravel slopes in forests, and in magma crevices around volcanoes. It is used to treat various skin diseases and arthritis, such as psoriasis, rash, dermatitis, beriberi and psoriasis, etc., but there are very few studies on its specific active ingredients, so it is urgently needed to be guided by traditional Chinese medicine theory and modern pharmacology and pharmacodynamics. Research and development of antibacterial drug ingredients of natural origin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound Disaspidin BB and application of compound Disaspidin BB in antibiosis
  • Compound Disaspidin BB and application of compound Disaspidin BB in antibiosis
  • Compound Disaspidin BB and application of compound Disaspidin BB in antibiosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0049] Example 1 Extraction and identification of Disaspidin BB

[0050] 1. Extraction method

[0051] The method of extracting the compound Disaspidin BB from Dryopteris serrata is as follows figure 1 As shown, it specifically includes the following steps:

[0052] S1. Extract extraction: 10kg dry dry powder of Dryopteris fragrantii was extracted twice with 10 times the volume fraction and 50% concentration of ethanol at room temperature for 2 hours, adjusted to pH 2.4 with hydrochloric acid, and soaked in ethanol The medicinal solution was concentrated and extracted to obtain 1.2 kg of extract;

[0053] S2. Preliminary separation of plant extract: After the sample is dissolved in ethyl acetate and methanol, the sample is mixed with silica gel, and the silica gel column is used for preliminary separation, followed by elution with different ratios of petroleum ether: acetone (100:1, 70: 1,50:1,30:1,10:1,1:1), and the corresponding components were named as components A (96g), B (320g)...

Embodiment 2D

[0068] Example 2 Disaspidin BB's inhibitory effect on fungi

[0069] 1. Experimental method

[0070] Use an inoculating loop to gently scrape the colonies on the surface of the SDA medium, grind the hyphae with a sterile grinder, suspend the dermatophytes in sterile saline, adjust the turbidity to 0.5 McDonnell’s turbidity, and count the spores with a hemocytometer Number and number of short hyphae. Make the concentration of the bacterial solution 1×10 3 CFU / mL to 3×10 3 CFU / mL. That is to use RPMI-1640 liquid medium to dilute the 0.5 McKenzie turbidity bacterial solution 1000 times, and count according to the hemocytometer to obtain the inoculation bacterial solution. The Dryopteris monomer stock solution (20μg / mL) diluted with RPMI-1640 liquid medium was placed in the first column of a 96-well plate, and the culture medium was used for horizontal 2-fold dilution to the 10th column. In the 11th column, 100μL RPMI- 1640 liquid medium, add 200μL RPMI-1640 liquid medium in column ...

Embodiment 3D

[0078] Example 3 Disaspidin BB's inhibitory effect on bacteria

[0079] 1. Experimental method

[0080] Test method: The micro-dilution method is used. This method is often used to test the antibacterial activity of sensitive drugs or new drugs against human pathogens. The operation method is simple and can be used for a large number of antibacterial tests. Generally, a nutrient broth medium is used, and the activated clinically pathogenic strains are inoculated into the medium to prepare a bacterial solution, and the drug to be tested is dissolved in DMSO to make up to 64 mg / mL. Dilute the drug to be tested line by line in a 96-well plate with a two-fold concentration-decreasing dilution method; then add the diluted bacterial solution, leaving two wells as a control, one with only medium and the other with only Add the bacteria solution, shake and mix evenly after the dilution, and then place the 96-well plate in a 35°C constant temperature incubator for 18-24 hours. The lowest c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicines, and particularly discloses a dryopteris fragrans compound Disaspidin BB and application of the dryopteris fragrans compound Disaspidin BB inthe antibacterial aspect. According to the invention, the phloroglucinol compound Disaspidin BB is separated and prepared from the dryopteris fragrans (L.) Schiott.), and the research results show that the compound has good antibacterial activity, especially has a very good antibacterial effect on dermatophyte and bacteria and is extracted from natural plants and has the characteristics of low toxic and side effects and low possibility of generating drug resistance and provides a new choice and way for developing antibacterial drugs and has a very good application prospect.

Description

Technical field [0001] The invention belongs to the field of medical technology. More specifically, it relates to Disaspidin BB, a compound of Dryopteris fragrantii and its application in antibacterial. Background technique [0002] In recent years, the incidence of clinical fungal infections has been on the rise, and fungal infections have attracted people's attention. According to the international medical standards, fungal infections can be roughly divided into three categories, namely, superficial mycoses, dermatophytes and systemic mycoses. The etiology of systemic fungal diseases is mostly due to impaired immune function of the body, and the disease is often serious and even life-threatening. There are still various deficiencies in commonly used antifungal drugs in clinical practice. For example, amphotericin B has serious adverse reactions, including nephrotoxicity, liver toxicity, etc.; fluconazole has good antibacterial activity against Candida albicans and Cryptococcu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C49/84A61K36/12A01N35/06A01P1/00A01P3/00
CPCC07C49/84A61K36/12A01N35/06
Inventor 沈志滨王帅帅
Owner GUANGDONG PHARMA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com