A kind of preparation method of strawberry fresh-keeping chitosan-based antibacterial film

The technology of chitosan and succinyl chitosan is applied in the field of preparation of chitosan-based antibacterial film for fresh-keeping strawberry, which can solve problems such as rot and mildew of strawberry, achieve prolonging shelf life, solve poor water solubility, and inhibit surface The effect of microbial growth

Active Publication Date: 2021-06-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of strawberry fresh-keeping chitosan-based antibacterial film, to solve the strawberry mildew, rot, fermentation, softening, oxidation and other problems that are easily caused after picking strawberries, and improve the storage period of strawberries.

Method used

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  • A kind of preparation method of strawberry fresh-keeping chitosan-based antibacterial film
  • A kind of preparation method of strawberry fresh-keeping chitosan-based antibacterial film
  • A kind of preparation method of strawberry fresh-keeping chitosan-based antibacterial film

Examples

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specific Embodiment 1

[0030] The preparation of N-succinyl chitosan (NSC) refers to CN 10651927A: chitosan powder is dissolved in DMSO according to the mass volume ratio of 1:18~1:22mg / mL, and succinic anhydride is slowly added to make chitosan and succinate The mass ratio of acid anhydride is 1~3, heat at 50~70°C for 6~8h, filter, dissolve the precipitate in ethanol, adjust the pH of the solution to 9~12 with 1M NaOH solution, filter the precipitate according to 1:50~ Dissolve in distilled water at a mass volume ratio of 1:55mg / mL, then add acetone, the mass ratio of acetone to distilled water is 3-5, wash the precipitate with ethanol and acetone, and dry the obtained product under vacuum at 50°C;

[0031] Dissolve 1.0g NSC in 20mL of phosphate buffer solution with pH=6, add 0.03gEDC and 0.03gNHS respectively, stir at room temperature for 3h, add glycerol with a volume fraction of 5%, stir and disperse evenly, and then Ultrasonic treatment was performed for 15 minutes to remove air bubbles to obta...

specific Embodiment 2

[0033] The preparation of N-succinyl chitosan (NSC) refers to CN 10651927A: chitosan powder is dissolved in DMSO according to the mass volume ratio of 1:18~1:22mg / mL, and succinic anhydride is added slowly, so that chitosan and succinate The mass ratio of acid anhydride is 1~3, heat at 50~70°C for 6~8h, filter, dissolve the precipitate in ethanol, adjust the pH of the solution to 9~12 with 1M NaOH solution, filter the precipitate according to 1:50~ Dissolve in distilled water at a mass volume ratio of 1:55mg / mL, then add acetone, the mass ratio of acetone to distilled water is 3-5, wash the precipitate with ethanol and acetone, and dry the obtained product under vacuum at 50°C;

[0034] Weigh 1.0g NSC and dissolve it in 20mL of phosphate buffer solution with pH=6, add 0.03gEDC and 0.03gNHS respectively, stir at room temperature for 3h, add glycerol with a volume fraction of 5%, stir for 30min, add a volume fraction of 2% Tween-80, continue to stir for 30min, add cinnamaldehyde...

specific Embodiment 3

[0036] The preparation of N-succinyl chitosan (NSC) refers to CN 10651927 A: Chitosan powder is dissolved in DMSO according to the mass volume ratio of 1:18~1:22mg / mL, slowly adds succinic anhydride, makes chitosan and The mass ratio of succinic anhydride is 1~3, heat at 50~70°C for 6~8h, filter, dissolve the precipitate in ethanol, adjust the pH of the solution to 9~12 with 1M NaOH solution, filter the precipitate according to 1:50 Dissolve in distilled water at a mass volume ratio of ~1:55mg / mL, then add acetone, the mass ratio of acetone to distilled water is 3~5, wash the precipitate with ethanol and acetone, and dry the obtained product under vacuum at 50°C;

[0037] Weigh 1.0g NSC and dissolve it in 20mL of phosphate buffer solution with pH=6, add 0.03gEDC and 0.03gNHS respectively, stir at room temperature for 3h, add glycerol with a volume fraction of 5%, stir for 30min, add a volume fraction of 2% Tween-80, continue to stir for 30 minutes, add cinnamaldehyde with a volu...

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Abstract

The invention discloses a preparation method of a strawberry fresh-keeping chitosan-based antibacterial film. Cinnamaldehyde was used as a bacteriostatic agent, and a natural antibacterial film with strong water solubility was prepared by casting method; experiments showed that cinnamaldehyde-chitosan-based antibacterial film had different effects on Staphylococcus aureus, Salmonella enteritidis, and Pseudomonas tolassii. Inhibition to a certain extent; fresh strawberries are sealed in a fresh-keeping box with an antibacterial film to achieve the effect of food preservation. Fresh strawberries can be kept fresh for 7‑8d at room temperature. The invention adopts chitosan-based bacteriostatic film to keep fresh strawberries fresh and store them. On the basis of no large-scale contact with strawberry fruits, it is safe, non-toxic, non-secondary pollution, green and environmentally friendly. It not only maintains the original quality of fruits and vegetables, but also significantly Extends the shelf life of fruits and vegetables.

Description

Technical field: [0001] The invention belongs to the technical field of fresh-keeping of fruits and vegetables, and relates to a preparation method of a chitosan-based bacteriostatic film for strawberry fresh-keeping. Background technique: [0002] N-succinyl chitosan (NSC) is a new type of water-soluble chitosan derivative developed in recent years, which is obtained by the phthalation reaction of chitosan and succinic anhydride. The N-acylation reaction of chitosan reduces the ability of chitosan to form intramolecular and intermolecular hydrogen bonds, and destroys the original regularity of chitosan molecular chains, resulting in a significant decrease in its crystallinity, so the water solubility of NSC Greatly improved, has good biocompatibility, low toxicity, and can stay in the human body for a long time. During the succinylation process, the original chitosan introduces a large number of carboxyl groups, which are activated by EDC and NHS and react with antibacteria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J5/18C08L5/08C08K5/07C08K5/053A23B7/00
CPCA23B7/00C08J5/18C08J2305/08C08K5/053C08K5/07
Inventor 王虹苏马永亮刘璐牛效迪
Owner JILIN UNIV
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