Long non-coding RNA LINC02481 related to gastric cancer and application of long non-coding RNA LINC02481

A LINC02481, long-chain non-coding technology

Active Publication Date: 2020-06-16
PEKING UNIV SHENZHEN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many patients with early gastric cancer lack specific clinical symptoms, and patient

Method used

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  • Long non-coding RNA LINC02481 related to gastric cancer and application of long non-coding RNA LINC02481
  • Long non-coding RNA LINC02481 related to gastric cancer and application of long non-coding RNA LINC02481
  • Long non-coding RNA LINC02481 related to gastric cancer and application of long non-coding RNA LINC02481

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] To detect the expression difference of LINC02481 in gastric cancer tissues and adjacent tissues

[0027] 1. Research object

[0028] Twenty gastric cancer tissues and 20 corresponding para-cancerous tissues were sourced from Qingdao Cancer Hospital. All tissue specimens were reviewed and approved by the hospital ethics committee. All patients from the tissue specimens signed informed consent. The gastric cancer tissue was confirmed to be gastric cancer by pathological examination (when the gastric cancer tissue was removed, the patient had not received chemotherapy or radiotherapy).

[0029] The specific organization is as follows

[0030]

[0031]

[0032] 2. Tissue RNA extraction

[0033] (1) Put 50mg of gastric cancer tissue or adjacent tissues into a mortar, add a small amount of liquid nitrogen, and grind quickly. After the tissue becomes soft, add a small amount of liquid nitrogen to grind again, repeat three times;

[0034] (2) Add 1mL Trizol, homogenize ...

Example Embodiment

[0067] Example 2

[0068] Fluorescence quantitative PCR to detect the expression of LINC02481 gene after knockdown

[0069] (1) Cell culture

[0070] Human gastric cancer cell MKN-45 was cultured in high-sugar DMEM medium (10% fetal bovine serum), placed at 37℃, 5% CO 2 Constant temperature cell incubator for cultivation.

[0071] (2) si-RNA transfection

[0072] (1) The day before transfection, 2×10 5 The MKN-45 cells were seeded in a 6-well plate, 37℃, 5% CO 2 Incubate in a constant temperature cell incubator for 24 hours, and follow the instructions of Lipofectamine2000 for transfection;

[0073] (2) The experiment was divided into control group, si-NC group, si-LINC02481-1, si-LINC02481siRNA-2, si-LINC02481-3.

[0074] 5'-AUUUGUCGGAGGCAUUAUCAA-3' (SEQ ID NO: 6)

[0075] 5’-GAUAAUGCCUCCGACAAAUAU-3’ (SEQ ID NO: 7)

[0076] si-LINC02481-2

[0077] 5'-GAAGGUGUUUGCUAGACAAAG-3' (SEQ ID NO: 8)

[0078] 5’-UUGUCUAGCAAACACCUUCUG-3’ (SEQ ID NO: 9)

[0079] si-LINC02481-3

[0080] 5'-UCAUGAUAAAUCGAACA...

Example Embodiment

[0088] Example 3

[0089] CCK-8 experiment detects the effect of transfection of si-LINC02481-2 on the proliferation of gastric cancer cells MKN-45

[0090] experimental method

[0091] (1) Put 2×10 3 The MKN-45 cells were seeded in a 96-well plate, 100μl per well, 3 multiple wells per group, 37℃, 5% CO2 incubator culture;

[0092] (2) MKN-45 cells were transfected with si-NC, si-LINC02481-2, 4h before the end of the culture at 24h, 48h, and 72h, add 10ul CCK-8 to each well, and after 4h incubation, 450nm Check the absorbance value.

[0093] Experimental results

[0094] by Figure 4 It can be seen that there is no significant difference between the si-NC group and the si-LINC02481-2 group at 24h of transfection, while at 48h and 72h (48h: si-NC 0.649±0.035, si-LINC02481-2 0.431±0.027; 72h: si-NC 1.057±0.415, si-LINC02481-2 0.683±0.039), the OD value of MKN-45 cells transfected with si-LINC02481-2 was significantly lower than that of MKN-45 cells transfected with si-NC , Indicating th...

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PUM

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Abstract

The invention aims to provide a long-chain non-coding RNA LINC02481 related to gastric cancer and an application of the long-chain non-coding RNA LINC02481. The long-chain non-coding RNA provided by the invention is highly expressed in gastric cancer tissues, so that the long-chain non-coding RNA can be used as a target for treating gastric cancer. The siRNA inhibitor designed according to the LINC02481 can inhibit the proliferation of gastric cancer cells and promote the apoptosis of the gastric cancer cells, so that the siRNA provided by the invention can be applied to the preparation of gastric cancer drugs. The invention has the beneficial effects that the invention provides a new target spot for gastric cancer diagnosis, and the detection has excellent diagnostic value; secondly, theinvention provides the application of the gene inhibitor of LINC02481 in preparation of the medicine for treating the gastric cancer, and a new direction is provided for development of the medicine for treating the gastric cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a long non-coding RNA LINC00248 related to gastric cancer and its application. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive system. Because the pathogenesis of gastric cancer is very complicated, and the early symptoms of patients are not obvious, the early diagnosis rate of gastric cancer patients is low. Gastric cancer is the fourth largest malignant tumor in the world. Although the incidence of gastric cancer has decreased in recent years, the mortality rate of patients has not been effectively reduced. In China, the mortality rate of gastric cancer patients still ranks second among all tumors. Gastric cancer is divided into early gastric cancer and advanced gastric cancer. Most gastric cancer patients are already in the advanced stage when they are diagnosed. Many patients with early gastric cancer lack specific cl...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K45/00A61K31/7088A61P35/00A61P1/00
CPCA61K31/7088A61K45/00A61P35/00C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王颖翠
Owner PEKING UNIV SHENZHEN HOSPITAL
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