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Hypersensitive cardiac troponin I detection method and kit

A cardiac troponin and detection kit technology, applied in the field of protein detection, can solve problems such as low detection rate, missed detection, and inability to meet changes in cardiac troponin content, and achieve the goal of increasing the detection rate and reducing missed detection risk effect

Active Publication Date: 2020-06-19
GUANGDONG WESAIL BIOTECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these products mostly use antibodies to a certain epitope to detect antigens, and there are generally missed detections and the detection rate of normal people cannot reach ≥ 50%.
Therefore, the performance of current kits generally does not meet the requirements of hypersensitivity, such as low detection rate, insufficient sensitivity and specificity, etc., cannot monitor the development trend of the disease, and cannot meet the requirements of detecting changes in cardiac troponin levels in blood of patients at different times.

Method used

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  • Hypersensitive cardiac troponin I detection method and kit
  • Hypersensitive cardiac troponin I detection method and kit
  • Hypersensitive cardiac troponin I detection method and kit

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Embodiment Construction

[0064] The present invention will be described in further detail below mainly in conjunction with specific embodiments. The following examples are provided to illustrate embodiments of the invention and are not intended to limit the invention. The invention may optionally include embodiments not shown by way of example.

[0065] Preparation of a cardiac troponin I monoclonal antibody

[0066] 1. Immune animals: take BALB / c mice of the same strain as myeloma cells at the age of 8 to 12 weeks, and fully use cTnI antigen (SEQ ID NO: 1) containing 100 μg of protein per mouse and the same amount of complete Freund's adjuvant. Mix well and inject into the abdominal cavity of mice. Every 2 weeks, 100 μg / cTnI antigen and the same amount of Freund's incomplete adjuvant are fully mixed and injected into the abdominal cavity of mice several times to strengthen the immunization. After the detection of mouse serum (indirect ELISA method), those whose titer is above 1:2000 can be used for...

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Abstract

The invention relates to a hypersensitive cardiac troponin I detection method and a kit. The provided hypersensitive cardiac troponin I detection kit comprises a first group of antibodies and a secondgroup of antibodies for detecting cardiac troponin I from a subject sample, and the antibodies are selected from 1-6 antibodies specifically binding to the following cTnI fragment: antibody 1: 27-60aa; an antibody 2: 69 to 86 aa; an antibody 3: 13 to 29 aa; an antibody 4: 25 to 40 aa; an antibody 5: 41 to 49 aa; and an antibody 6: 169 to 178 aa. The method and the kit disclosed by the inventionare high in sensitivity and simple to operate, and can be used for dynamically monitoring the cardiac troponin I.

Description

technical field [0001] The invention relates to the field of protein detection. Specifically, the present invention relates to a detection method and a kit for hypersensitive cardiac troponin I. Background technique [0002] Elevation of blood cardiac troponin I (cTnI: cardiac troponin I) has very high sensitivity and specificity for diagnosing myocardial injury / necrosis (myocardial necrosis). At present, cTnI has become an essential marker for the clinical diagnosis, treatment and monitoring of myocardial infarction. [0003] Cardiac troponin exists in a small amount in normal people, but when myocardial infarction occurs, myocardial cell damage causes cTnI to be released into the blood, no matter whether the ST segment is elevated or not, cTnI will rise sharply, reaching the peak at about 24 hours, and after 7-10 days dropped to normal levels. There is a certain degree of correlation between the cTnI elevation and the degree of myocardial cell necrosis. If the cTnI in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577C07K16/18
CPCC07K16/18C07K2317/34G01N33/577G01N33/6887G01N33/6893G01N2333/4712G01N2800/324
Inventor 刘丽萍于鸫黄春连迟晨何智丹李然王凯李斌李文静卢盛萍
Owner GUANGDONG WESAIL BIOTECH CO LTD
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