Optical system of gene sequencer

Abstract

The invention discloses an optical system of a gene sequencer. The optical system comprises a light source assembly, a light path assembly and a camera, wherein the light source assembly is used for emitting laser; the light path assembly comprises a dichroscope, an objective, an optical splitter and a condenser; the optical splitter is mounted in a light path of fluorescence transmitted by the dichroscope and used for separating fluorescence at different wavelengths; the condenser is mounted in a light path of fluorescence split by the optical splitter and used for focusing the separated fluorescence; and a light-sensitive surface of the camera is adjustably fixed to the focus surface of the condenser and used for collecting fluorescence focused by the condenser to generate correspondingimages. The optical splitter and the condenser are mounted between the dichroscope and the camera, and the fluorescence which comprises multiple different wavelengths and is transmitted by the dichroscope can be split by the optical splitter and focused on the camera by the condenser, so that collection and imaging of fluorescence at different wavelengths can be completed by one dichroscope and one camera, the sequencing requirement can be met, the light path is simpler, and the cost is lower.

Description

technical field [0001] The present application relates to the technical field of gene sequencing, in particular to an optical system of a gene sequencer. Background technique [0002] Gene sequencing (including DNA sequencing and RNA sequencing) is one of the important methods for studying nucleic acids. DNA sequencing refers to the analysis of the base arrangement of a specific DNA fragment, that is, the arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G). Similarly, RNA sequencing refers to the analysis of the base sequence of a specific RNA fragment, that is, the arrangement of adenine (A), uracil (U), cytosine (C) and guanine (G). [0003] At present, the method of fluorescent labeling is generally used for gene sequencing, and a laser illumination light path and a fluorescence collection light path need to be set in the gene sequencer to excite and collect fluorescence signals respectively. [0004] The optical system of the gene sequencer in the pri...

AI Technical Summary

Problems solved by technology

[0004] The optical system of the gene sequencer in the prior art uses multiple dichroic lenses to collect fluorescence of different wavelengths into multiple acquisition cameras, the optical structure is relatively complex, and the cost of using multiple acquisition cameras at the same time is high, resulting in high development costs

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