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Method, medium and biological antibacterial agent for cultivating Streptomyces albicans

A biological bacteriostatic agent, the technology of Streptomyces fumigatus, applied in the biological field, can solve the problems such as poor bacteriostatic effect of Streptomyces leucoides bacterial solution

Active Publication Date: 2022-05-06
陕西赛恩农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But at present, the bacteriostatic effect of the Streptomyces albicans bacterial liquid cultivated by adopting the existing culture method is relatively poor.

Method used

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  • Method, medium and biological antibacterial agent for cultivating Streptomyces albicans
  • Method, medium and biological antibacterial agent for cultivating Streptomyces albicans
  • Method, medium and biological antibacterial agent for cultivating Streptomyces albicans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The method for cultivating Streptomyces albicans provided by the present embodiment may further comprise the steps:

[0041] (1) Strain activation

[0042] Take the frozen Streptomyces albicans (preservation number is CGMCC No.13717), inoculate it on a plate, incubate at a constant temperature of 26°C for 10 days, and inoculate 2 pieces of bacteria blocks taken with a hole punch with a diameter of 5mm in a 100mL liquid culture container. In a Erlenmeyer flask (250mL size) based on the base, the culture was shaken on a shaker, the culture conditions were 26°C, 180r / min, and the seed liquid was obtained after culturing for 7 days. The solid medium used is the same as the liquid medium except for the addition of agar. The formula is as follows: glucose 10g, soluble starch 10g, yeast extract 5g, soybean powder 10g, peptone 5g, CaCO 3 1.5g, NaCl 1g, MgSO 40.5g, K 2 PO 4 0.3 g and 1000 mL of water.

[0043] (2) Inoculation culture

[0044] The seed liquid was inocula...

Embodiment 2

[0047] The method for cultivating Streptomyces albicans in this example is basically the same as in Example 1, except that the initial pH of the culture medium is 7.5.

Embodiment 3

[0049] The method for cultivating Streptomyces albicans in this example is basically the same as that in Example 1, except that the inoculum size is 15%.

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Abstract

The invention discloses a method for cultivating Streptomyces albicans, a culture medium and a biological antibacterial agent, and relates to the field of biological technology. The method for cultivating Streptomyces microalbicans, which is disclosed in the present invention, comprises inoculating Streptomyceses microalbicans in a mixture containing glucose, soluble starch, yeast extract, soybean powder, peptone, CaCO 3 , NaCl, MgSO 4 and K 2 PO 4 culture medium. Adopting the culture method provided by the present invention to cultivate Streptomyces microalbicans can make the cultivated Streptomyces microalbsus bacterium liquid have stronger bacteriostasis effect, and provide a method for large-scale cultivation of Streptomyces microalbinus to produce biological antibacterial agents guide.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating Streptomyces albicans, a culture medium and a biological antibacterial agent. Background technique [0002] In the biological control of plant diseases, actinomycetes have been paid more and more attention because of their wide practical applications. However, most of the actinomycetes used for biological control are screened from soil or plant rhizosphere. Because they are easily affected by external conditions, the control effect is unstable, which greatly weakens the application value. [0003] As a new microbial resource, plant endophytic actinomycetes have a wide distribution, many types, and a stable living environment. They can colonize the host plant and occupy a favorable biocontrol position to avoid direct competition with microorganisms in the external environment. , thereby overcoming the limitations of other exogenous actinomycetes. Endophytic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCC12N1/20
Inventor 吴爱茹陈琳李红超
Owner 陕西赛恩农业科技股份有限公司