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Radix ophiopogonis detection method

A detection method, Ophiopogon japonicus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult comparison and judgment, unclear spots, etc.

Pending Publication Date: 2020-06-30
HEBEI WANBANG FOLON PHARMA
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  • Abstract
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Problems solved by technology

[0002] Ophiopogon japonicus is recorded in the 2015 edition of "Chinese Pharmacopoeia", the original quality standard of Ophiopogon japonicus identification item only has the control medicinal material as a reference, using silica gel GF 254 When the plate is unfolded and detected, when observed under the ultraviolet light of 254nm, the spots are occasionally not clear, and it is difficult to compare and judge

Method used

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  • Radix ophiopogonis detection method
  • Radix ophiopogonis detection method
  • Radix ophiopogonis detection method

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preparation example Construction

[0032] ①Preparation of the reference medicinal solution

[0033] Take 3 g of Ophiopogon japonicus reference medicinal material, add 50 ml of methanol, heat and reflux for 2 h, let it stand still, take the supernatant and evaporate to dryness, add 40 ml of water to dissolve the residue, add 1 ml of hydrochloric acid, boil slightly, heat and reflux for 1 h, cool, and transfer to a separatory funnel, Extract 3 times with n-butanol saturated with water, 40ml each time, combine the extracts, wash 2 times with water, 50ml each time, discard the water washing solution, recover n-butanol to dryness, add 2ml methanol to the residue to dissolve, and use it as a reference drug solution .

[0034] ② Preparation of reference solution

[0035] Accurately weigh 5 mg of ruscogenin, add 5 ml of methanol to make a solution containing 1 mg per 1 ml, as the reference solution.

[0036] ③ Preparation of the test solution

[0037] Take 3g of Ophiopogon japonicus medicinal material, add 50ml of m...

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Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a radix ophiopogonis detection method. According to the method disclosed by the invention, the luscogenin is detected by adopting a thin-layer chromatography identification method, so that the technical problem of a radix ophiopogonis identification method is solved. The method is high in durability, and the product quality of the radix ophiopogonis can be more effectively monitored.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine. It specifically relates to a detection method for the identification of the Chinese medicinal material Ophiopogon japonicus by thin-layer chromatography. Background technique [0002] Ophiopogon japonicus is recorded in the 2015 edition of "Chinese Pharmacopoeia", the original quality standard of Ophiopogon japonicus identification item only has the control medicinal material as a reference, using silica gel GF 254 When the plate is unfolded for detection, when observed under the ultraviolet light of 254nm, the spots are occasionally not clear, and it is difficult to compare and judge. In this method, a ruscogenin reference substance is added as a reference, and the silica gel G plate is used to develop, and the spots are clearly identifiable, which can better control the quality of medicinal materials, and the use of chloroform is removed in this method, which is more envir...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 孙秋亚江廷峰李东慧
Owner HEBEI WANBANG FOLON PHARMA