Application of Cynanoside H in preparation of drug for preventing and treating breast cancer

A technology of breast cancer, breast cancer cells, applied in the field of medicine

Active Publication Date: 2020-07-03
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cynanoside H is a difidopregnane-type steroid compound isolated from the root of Baiwei root. It has been reported that Cynanoside H has significant anti-tobacco mosaic virus (TMV) activity, but there is no report about the pharmacological effects of Cynanoside H.

Method used

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  • Application of Cynanoside H in preparation of drug for preventing and treating breast cancer
  • Application of Cynanoside H in preparation of drug for preventing and treating breast cancer
  • Application of Cynanoside H in preparation of drug for preventing and treating breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Cell Viability Test

[0050] Collect logarithmic phase cells, MDA-MB-231 cells according to 6×10 3 The density of each well was planted in a 96-well plate and cultured overnight. After the cells adhered to the wall, they were treated with different concentrations of Cynanoside H (hereinafter referred to as BW18), and 0.1% DMSO was used as the control group. After 72 hours of drug action, add 20 μL of MTT with a concentration of 5 mg / mL to each well, incubate at 37°C for 4 hours, discard the supernatant, add 160 μL DMSO to each well to dissolve, and measure the absorbance value OD with a microplate reader after the blue-purple crystal formazan is completely dissolved. (490nm).

[0051] Calculation method of inhibition rate: inhibition rate=(OD490nm of control group-OD490nm of treatment group) / OD490nm of control group. half inhibition rate (IC 50 ) calculation method: use the forecast function to obtain the IC corresponding to the compound 50 .

[0052] The results s...

Embodiment 2

[0054] Observation of cell morphology

[0055] Collect logarithmic phase cells, MDA-MB-231 cells according to 4×10 3 The density of each well was planted in a 96-well plate and cultured overnight. After the cells adhered to the wall, high, medium and low (10 μM, 5 μM and 2.5 μM) concentrations of BW18 were added to treat MDA-MB-231 cells, and 0.1% DMSO for the control group. After 72 h, the morphological changes of the cells were observed.

[0056] The result is as figure 2 As shown, with the increase of BW18 concentration, the number of MDA-MB-231 cells decreased significantly. 5 μM and 10 μM BW18 significantly inhibited cell growth, and after BW18 acted on MDA-MB-231 cells, the cell shape changed from long spindle to round.

Embodiment 3

[0058] Cell Growth Curve Determination

[0059] Collect logarithmic phase cells, MDA-MB-231 cells according to 4×10 3 The density of cells / well was planted in a 96-well plate and cultured overnight. After the cells adhered to the wall, they were treated with high, medium and low (10 μM, 5 μM and 2.5 μM) concentrations of BW18 at 0 h, 12 h, 24 h, 36 h, respectively. OD490nm was measured at 48h, 72h and 96h, and the growth curve was drawn.

[0060] The result is as image 3 As shown, compared with the control group, different concentrations of BW18 can inhibit the proliferation of MDA-MB-231 cells, and with the increase of BW18 concentration, the growth rate of MDA-MB-231 cells gradually slowed down, indicating that BW18 can exhibit Inhibits the proliferation of MDA-MB-231 in a concentration- and time-dependent manner.

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Abstract

The invention relates to the technical field of medicines, in particular to an application of Cynanoside H in preparation of a drug for preventing and treating a breast cancer. A pharmacological studyshows that Cynanoside H inhibits MDA-MB-231 cell proliferation through inducing S-phase cycle arrest, reverses epithelial-mesenchymal transition to inhibit MDA-MB-231 cell migration and invasion, plays a role in preventing and treating the breast cancer, especially triple-negative breast cancer, and provides a new idea for research and development of the drug for preventing and/or treating the breast cancer.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Cynanoside H in the preparation of drugs for preventing and treating breast cancer. Background technique [0002] According to the Global Cancer Statistical Report (GLOBOCAN) released by the American Cancer Society, there were approximately 2.1 million new breast cancer cases worldwide in 2018, accounting for 11.6% of all new cancer cases, 24.2% of new cancer cases in women, and breast cancer deaths Accounting for 15% of female cancer deaths, it is the tumor disease with the highest morbidity and mortality among female cancers in the world. Breast cancer, like other tumors, is a heterogeneous disease with complex pathogenesis. At present, breast cancer treatment methods mainly include surgery, radiotherapy and chemotherapy, endocrine therapy and targeted therapy. But it is worth noting that triple-negative breast cancer patients account for about 15% of brea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00
CPCA61K31/7048A61P35/00
Inventor 杨珏李艳梅郝小江晏英邱剑飞饶青宋晶睿
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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