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Ganoderma lucidum fruiting body composite probiotic preparation for enhancing organism immunity

A technology of Ganoderma lucidum fruiting body and compound probiotics, which is applied in the field of Ganoderma lucidum fruiting body compound probiotic preparations to achieve the effects of enhancing immunity, improving immunity, and improving low utilization rate

Inactive Publication Date: 2020-07-03
合肥康春堂药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] How to rationally use the co-fermentation of Ganoderma lucidum fruiting body and probiotics to prepare a compound probiotic preparation of Ganoderma lucidum fruiting body for enhancing the body's immunity is a topic worthy of further research, and there are no relevant literature reports so far.

Method used

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  • Ganoderma lucidum fruiting body composite probiotic preparation for enhancing organism immunity
  • Ganoderma lucidum fruiting body composite probiotic preparation for enhancing organism immunity
  • Ganoderma lucidum fruiting body composite probiotic preparation for enhancing organism immunity

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Experimental program
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Effect test

Embodiment 1

[0029] A preparation method of a ganoderma lucidum fruiting body compound probiotic preparation for enhancing body immunity, the steps are as follows:

[0030] 1) Bacterial strain screening: through OD value detection, strains that can grow on Ganoderma lucidum medium are screened, including Lactobacillus acidophilus, Bifidobacterium breve, Lactobacillus 9218 breve, Lactobacillus casei, Bifidobacterium pseudosmall chain , Lactobacillus gasseri.

[0031] 2), preparation of MRS medium containing three concentrations of Ganoderma lucidum extract in low, medium and high concentrations

[0032] Ganoderma lucidum fruiting body powder was prepared with water into Ganoderma lucidum extracts with three concentrations of 8.8mg / ml, 16.6mg / ml, and 33.2mg / ml, and then added to MRS medium containing 0.5% sugar respectively, and processed under high pressure to produce MRS medium containing Ganoderma lucidum extract.

[0033] 3), inoculation, fermentation

[0034] The above-mentioned vari...

Embodiment 2

[0038] Determination of the growth and total sugar content of probiotics in the probiotic fermentation broth of Ganoderma lucidum fruiting bodies: measure the OD value at 620nm and draw the growth curve to obtain the growth of probiotics; use the phenol-concentrated sulfuric acid method to understand the decomposition rules of sugars in the fermentation broth, Then determine the appropriate concentration of Ganoderma lucidum medium. Mass spectrometry analysis was performed on the compound probiotic fermentation preparation to detect whether there was a composition change before and after fermentation. The specific steps for determining the total sugar content are as follows:

[0039] Taking glucose as the standard product, using the phenol-concentrated sulfuric acid method, take 6 clean test tubes and number them, draw 0, 0.2, 0.4, 0.6, 0.8, 1.0ml of 0.1mg / ml standard glucose solution into the test tubes, and then make up with distilled water To 1ml, add 1ml of 6% phenol and ...

Embodiment 3

[0044] Application of compound fermentation liquid of Ganoderma lucidum fruiting bodies-Lactobacillus acidophilus and Bifidobacterium breve in animal models of hypoimmunity.

[0045] Mainly observe the changes in animal weight and feces; pathological detection of colon tissue HE staining; ELISA double antibody sandwich method to detect IL-17, TNF-α; PCR-denaturing gradient gel electrophoresis method to detect the structure of intestinal flora in mice. The specific steps are:

[0046] Male Balb / c mice aged 6-8 weeks were used to construct the animal model, and were divided into 4 groups (8 mice in each group), which were control (CON) group, dexamethasone model group (DEX) group, fermentation broth (FT ) group, Ganoderma lucidum (GL) group. Except for the CON group, the mice in the other three groups were intraperitoneally injected with dexamethasone 20 mg / (kg.d) daily for 7 consecutive days to establish a model of hypoimmunity. From the 8th day, 0.2ml of Ganoderma lucidum fe...

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Abstract

The invention relates to the field of fermentation engineering, in particular to a ganoderma lucidum fruiting body composite probiotic preparation for enhancing organism immunity. A fermentation product is obtained by co-fermentation of a ganoderma lucidum fruiting body extracting solution and a composite probiotics strain, wherein the composite probiotics comprise lactobacillus acidophilus and bifidobacterium breve in a number ratio of viable bacteria of 1 to 1; the ganoderma lucidum fruiting body powder is prepared into the ganoderma lucidum extracting solution with the concentration of 8.8-33.2mg / ml, and then the extracting solution is added into an MRS culture medium with 0.5% of sugar. Next, the culture medium is inoculated with the lactobacillus acidophilus and bifidobacterium breveat the concentration of 1% separately, and culturing is carried out at 37 DEG C for 48h for fermentation to obtain the fermentation product. The preparation can relieve the phenomenon of low immunityand enhance the immunity of the organism. Meanwhile, the problem of low utilization rate of the precious Chinese herbal medicine which refers to the ganoderma lucidum can be relieved.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a ganoderma lucidum fruiting body composite probiotic preparation for enhancing body immunity. Background technique [0002] Ganoderma lucidum (scientific name: Ganoderma Lucidum Karst), which is umbrella-shaped in shape, with a kidney-shaped, semicircular or nearly round cap, is the fruiting body of Ganoderma lucidum, a polyporaceae fungus. Ganoderma lucidum has different effects on neurasthenia, hyperlipidemia, coronary heart disease, angina pectoris, arrhythmia, altitude sickness, hepatitis, hemorrhagic fever, indigestion, and bronchitis. [0003] Fruiting body is the spore-forming structure of higher fungi, that is, the fruiting body, which is composed of organized mycelium and is the main edible and medicinal part of edible and medicinal fungi. Ganoderma lucidum fruiting body powder refers to the powder of Ganoderma lucidum fruiting body pulverized by ultra-fine crus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P37/04
CPCA61K36/074A61K2236/19A61P37/04
Inventor 孔振贾尚一李明刘赫袁杰力
Owner 合肥康春堂药业有限责任公司
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