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Method for fermenting ginseng with Lactobacillus fermentum, ginseng fermentation product and application

A technology for fermenting lactobacillus and fermenting ginseng is applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., and can solve problems such as the production of ginsenosides that are not involved

Active Publication Date: 2022-04-01
江苏菌钥生命科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method makes the output of ginseng fermented liquid Rh1+Rg2, F1, Rh2, 20(S)-Rg3, R0 all greatly improved, but above-mentioned fermentation condition does not relate to ginsenoside Rg1, Re, Rb1, Rc, Rd Yield increase

Method used

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  • Method for fermenting ginseng with Lactobacillus fermentum, ginseng fermentation product and application
  • Method for fermenting ginseng with Lactobacillus fermentum, ginseng fermentation product and application
  • Method for fermenting ginseng with Lactobacillus fermentum, ginseng fermentation product and application

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Embodiment approach

[0040] According to a preferred embodiment of the present invention, the method for fermenting ginseng with Lactobacillus fermentation comprises the following steps: (1) adding water to ginseng powder to prepare ginseng homogenate, and sterilizing it to obtain ginseng sterilized liquid; wherein, the amount of ginseng added in the ginseng homogenate is 8~10wt%; (2) Inoculate Lactobacillus fermentium (Lactobacillus fermentium) seed solution after the ginseng sterilization solution is cooled, carry out shaker fermentation under the fermentation temperature of 26~32 ℃, shaker speed is 150~200rmp, ferment The time is 40 to 45 hours to obtain the ginseng fermentation product; wherein, the Lactobacillus fermentum seed liquid is the Lactobacillus fermentum seed liquid prepared by using MRS liquid medium; the number of viable bacteria in the Lactobacillus fermentum seed liquid is 10 7 ~10 8 CFU mL -1 ; The inoculum amount of the Lactobacillus fermentum seed liquid is 2.5-3.5 wt% of th...

Embodiment 1

[0059] The method for fermenting ginseng by lactobacillus fermentation comprises the following steps:

[0060](1) Ginseng is crushed through the No. 4 pharmacopoeia sieve to obtain ginseng powder; the ginseng powder is suspended with deionized water to prepare a ginseng homogenate, which is sterilized by high-pressure steam at a temperature of 121°C. The time is 20 minutes to obtain ginseng sterilized liquid; wherein, the amount of ginseng added in the ginseng homogenate is 8wt%.

[0061] (2) Inoculate the Lactobacillus fermentium (Lactobacillus fermentium) seed liquid after the above-mentioned ginseng sterilization liquid is cooled, and the viable count of the Lactobacillus fermentium seed liquid is 5×10 7 CFU mL -1 , the inoculum amount of Lactobacillus fermentum seed solution is 3wt% of the weight of the ginseng sterilization solution, the shaker fermentation is carried out at 28°C, the shaker speed is 160rmp, the fermentation time is 36h, and the ginseng fermentation prod...

Embodiment 2

[0063] The method for fermenting ginseng by lactobacillus fermentation comprises the following steps:

[0064] (1) Ginseng is crushed through the No. 4 pharmacopoeia sieve to obtain ginseng powder; the ginseng powder is suspended with deionized water to prepare a ginseng homogenate, which is sterilized by high-pressure steam at a temperature of 121°C. The time is 20 minutes to obtain the ginseng sterilized liquid; wherein, the amount of ginseng added in the ginseng homogenate is 8.03wt%.

[0065] (2) Inoculate the Lactobacillus fermentium (Lactobacillus fermentium) seed liquid after the above-mentioned ginseng sterilization liquid is cooled, and the viable count of the Lactobacillus fermentium seed liquid is 5×10 7 CFU mL -1 , the inoculum amount of the Lactobacillus fermentum seed solution is 2wt% of the weight of the ginseng sterilization solution, and the shaker fermentation is carried out at 28.24° C., the shaker speed is 160rmp, and the fermentation time is 42h to obtain...

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Abstract

The invention discloses a method for fermenting ginseng by fermenting lactobacillus, a ginseng fermentation product and its application. The method for fermenting ginseng with lactobacilli comprises the following steps: (1) adding water to ginseng powder to prepare ginseng homogenate, and sterilizing to obtain ginseng sterilized liquid; wherein, the amount of ginseng added to the ginseng homogenate is 5-12 wt %; (2) ) after cooling the ginseng sterilizing liquid, inoculate Lactobacillus fermentum seed liquid, carry out shaker fermentation at a fermentation temperature of 24-36°C, the shaker speed is 100-300rmp, and the fermentation time is 20-50h, to obtain a ginseng fermentation product. The method of the invention can increase the output of ginsenosides Rg1, Re, Rb1, Rc and Rd in ginseng.

Description

technical field [0001] The invention relates to a method for fermenting ginseng by fermenting lactobacillus, a ginseng fermentation product and its application. Background technique [0002] Ginseng is a perennial herbaceous plant of Araliaceae. In 2012, the Ministry of Health approved ginseng (ginseng cultivated artificially for 5 years or less) as a new resource food. Modern medicine has proved that ginseng has many biologically active functions. Ginseng is not only anti-cancer, anti-aging, anti-oxidation, but also effectively improves the body's immune regulation system. [0003] The chemical composition of ginseng is very complex, including saponins, volatile oils, sugars and vitamins. Modern medical and pharmacological studies have proved that ginsenoside is the main active ingredient of ginseng. The roots, stems, leaves, flowers and fruits of ginseng contain various ginsenosides. So far, it has been reported in the literature that ginsenosides that have been isolate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12P33/00C12N1/20A61P39/06C12R1/225
CPCC12P33/20C12P33/00C12N1/20A61P39/06
Inventor 林峰史新元马涛宋帅牛亚冰屈青松赵崇妍
Owner 江苏菌钥生命科技发展有限公司