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Design of new anti-cancer peptides and application of anti-cancer peptides in preventing tumors

An anti-cancer peptide, anti-tumor technology, applied in the direction of anti-tumor drugs, peptide preparation methods, peptides, etc.

Active Publication Date: 2020-07-07
孙治刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, resistance to many anticancer drugs has emerged

Method used

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  • Design of new anti-cancer peptides and application of anti-cancer peptides in preventing tumors
  • Design of new anti-cancer peptides and application of anti-cancer peptides in preventing tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Characterization of SZG3 and SZG5

[0013] SZG3: ESI-MS, calc. for [M] 1450.67, found 1450.62.

[0014] SZG5: ESI-MS, calc. for [M] 1687.01, found 1687.23.

Embodiment 2

[0015] Example 2: Cytotoxicity experiments and anti-tumor cell experiments of SZG3 and SZG5

[0016] 1. Experimental materials and methods

[0017] Hela cells and 293T cells in the logarithmic growth phase were used, and the cell concentration was adjusted to 5×10 with a culture medium containing 10% fetal bovine serum. 4 Cells / mL, seeded into 96-well plate. After culturing for 12 hours, different concentrations of polypeptides were added, and 4 replicate wells were set up for each polypeptide concentration, and a blank control was set up. The absorbance at a wavelength of 490 nm was measured by the MTT method.

[0018] 2. Experimental results

[0019] IC 50 (μM)-Hela

[0020] The results showed that the IC50 values ​​of SZG3 and SZG5 on Hela cells were 68.54 μM and 68.73 μM, respectively, while their toxicity to 293T cells was low. This indicates that SZG3 and SZG5 have good targeting and inhibitory effects on tumor cells.

Embodiment 3

[0021] Example 3: Hemolytic experiments of SZG3 and SZG5

[0022] 1. Experimental materials and methods

[0023] The hemolysis rate of SZG3 and SZG5 was determined by microplate reader.

[0024] A 1000 μg / mL target antimicrobial peptide solution was prepared with 0.9% physiological saline, and then double-diluted into a 96-well plate, and each sample was parallelized 3 times. Add fresh healthy rabbit blood to a 1g / L sodium heparin centrifuge tube, centrifuge at 1000rpm for 5 minutes, and discard the supernatant. Wash twice with 0.9% normal saline, discard the supernatant to obtain packed red blood cells. Take 3 drops of packed red blood cells and add them to 6 mL of normal saline to obtain a 2% red blood cell suspension. Take 50 μL of 2% erythrocyte suspension and add it to the above-mentioned 96-well plate. Use physiological saline containing 2% red blood cells and distilled water as negative control and positive control respectively. After incubating on a shaker at 37° ...

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Abstract

Two anti-cancer peptides with anti-tumor effects have the following amino acid sequences. Experiments show that SZG3 and SZG5 have very good selectivity and inhibitory activities on tumor cells. The present invention discloses the amino acid sequences and antitumor activities of the two anti-cancer peptides. The amino acid sequence of the SZG3 is MAMVGTRHHHHH and the amino acid sequence of the SZG5 is MWAIVLLRHHHHH.

Description

technical field [0001] The present invention relates to the design of novel anticancer peptide and its application in antitumor. Background technique [0002] Tumor is a complex disease caused by multiple factors. When certain cell gene mutations in the human body reach a certain level, the body will lose its normal regulation of its growth and form a tumor. Cancer is the most common malignant tumor, such as liver cancer, lung cancer, gastric cancer, cervical cancer, breast cancer, prostate cancer and so on. According to the latest global statistics, nearly 9 million people lost their lives due to cancer in a year, which is equivalent to 22,000 cancer patients dying every day. In the future, the global cancer burden will continue to increase due to world population growth and population aging. By 2030, there will be 21 million new cancer patients and 13 million cancer deaths every year. my country is a big cancer country. The number of cancer cases in China continues to r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/04A61P35/00
CPCC07K7/08A61P35/00
Inventor 孙治刚
Owner 孙治刚
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