A method for systematically monitoring and purifying Pseudomonas aeruginosa during incubation

A systemic technology of Pseudomonas aeruginosa, applied in the field of poultry breeding, can solve the problems of complex preparation, economic loss, excessive raw materials, etc., and achieve the effects of accurate sampling results, guaranteeing cleanliness, and shortening the time period

Active Publication Date: 2021-10-29
BEIJING POULTRY BREEDING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned disinfectant has too many raw materials in the process of preparation, and the preparation is complicated
[0007] This kind of disinfection process undoubtedly brings a lot of workload to the hatching work of the eggs, and in the case of many operations, it will also cause economic losses due to bacterial infection of the chicks.

Method used

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  • A method for systematically monitoring and purifying Pseudomonas aeruginosa during incubation
  • A method for systematically monitoring and purifying Pseudomonas aeruginosa during incubation
  • A method for systematically monitoring and purifying Pseudomonas aeruginosa during incubation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The invention provides a method for systematically monitoring and purifying Pseudomonas aeruginosa in the hatching process, said method comprising the following steps:

[0043] S1 Use disinfectant A to disinfect the eggs, incubators, hatching halls, hatchers and hatching halls before hatching. Wherein, disinfectant A is the mixed solution that forms after adding glutaraldehyde solution, ciprofloxacin and enoxistrobin to 75% ethanol solution, wherein the mass fraction of glutaraldehyde solution is 1.09%, the mass fraction of exifloxacin is 0.7%, the mass fraction of fenoxastrobin is 7%, and the mass fraction of 75% ethanol solution as a solvent is 91.21%. The ratio is mixed and then stirred evenly. The hatching process of the eggs is then carried out.

[0044] The configuration method of disinfectant A is: (1) configuration obtains the ethanol solution with a volume fraction of 75%, for subsequent use; prepares powdered ciprofloxacin and enoxastrobin for subsequent use...

Embodiment 2-7

[0062] The difference between Examples 2-7 and Example 1 is that the formula ratio of disinfectant A is different, see Table 3 for details, and the others are the same as Example 1. After the unqualified samples appear in Examples 2-7, the disinfection operation for the corresponding samples is the same as in Example 1.

[0063] The formula ratio of the disinfectant A of table 3 embodiment 1-7

[0064]

Embodiment 8

[0066] The difference between this embodiment and embodiment 1 is:

[0067] The method of using disinfectant A in step S1 is: spray the disinfectant A on the hatching hall and the incubator, spray for 10 minutes, and then fumigate for 10 minutes to complete the disinfection of the hatching hall; then spray the disinfectant A on the hatching eggs and eggs. After spraying on the surface of the tray for 10 minutes, fumigate for another 10 minutes to complete the disinfection of eggs and egg trays. Others are with embodiment 1.

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Abstract

The invention discloses a method for systematically monitoring and purifying Pseudomonas aeruginosa in the hatching process, which belongs to the technical field of poultry breeding. Detect the amount of Pseudomonas aeruginosa in the air and water in the eggs, incubators and hatching halls, and only pass it; during S2 incubation, test the content of Pseudomonas aeruginosa in the air in the hatching hall, and pass it; Check the content of Pseudomonas aeruginosa in the air in the chick hall and the surface of the hatcher, and enter the next process after passing the test; when the Pseudomonas aeruginosa test fails, disinfect the sample or the environment again; the disinfectant A is mixed with the following mass percentage raw materials Formation: glutaraldehyde 0.97~1.21%, epoxyfloxacin 0.5~0.9%, enoxastrobin 5~9%, and the balance is 75% ethanol solution. The method of the invention has the advantages of effectively and quickly disinfecting the Pseudomonas aeruginosa in the hatching process and ensuring a sterile hatching environment.

Description

technical field [0001] The invention relates to the technical field of poultry breeding, more specifically, it relates to a method for systematically monitoring and purifying Pseudomonas aeruginosa in the hatching process. Background technique [0002] Pseudomonas aeruginosa, also known as Pseudomonas aeruginosa (Pseudomonas aeruginosa), belongs to a Gram-negative bacillus of the genus Pseudomonas. Pseudomonas aeruginosa is widely distributed in nature. This bacteria exists everywhere. Chicken and turkey are the most common poultry hosts, and the egg-soaking solution may also contaminate the bacteria. The cracking of rotten eggs in the incubator may be a source of Pseudomonas aeruginosa infection outbreaks in chicks. Chicks have the highest susceptibility to Pseudomonas aeruginosa, and as the age increases, the susceptibility becomes lower and lower; if the incubator is contaminated by Pseudomonas aeruginosa, there will be burst eggs during the hatching process, and at the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A01N35/02A01N43/60A01N37/50A01P1/00A61L2/22A61L2/28A61L9/14C12Q1/06C12R1/385A61L101/34A61L101/44A61L101/32
CPCA01K67/02A01N35/02A01N37/50A01N43/60A61L2/22A61L2/28A61L9/14A61L2209/21C12Q1/06
Inventor 沈宇宁郑云平孙松松
Owner BEIJING POULTRY BREEDING
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