Check patentability & draft patents in minutes with Patsnap Eureka AI!

Trichoderma asperelloides with salt and alkali tolerance and application of Trichoderma asperelloides

A technology of Trichoderma aculeatus and salt-alkali resistance, which is applied in the biological field and can solve the problems of few microbial groups, difficulty in rooting and standing of crops, poor physical and chemical properties, etc.

Active Publication Date: 2020-07-10
NANJING AGRICULTURAL UNIVERSITY
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low content of organic matter in saline soil, poor physical and chemical properties, few microbial groups, many anions and cations harmful to crops, and high osmotic pressure of soil solution, it is difficult for crops to take root and stand up. Therefore, how to promote the growth of plant roots in saline soil is the key technology.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trichoderma asperelloides with salt and alkali tolerance and application of Trichoderma asperelloides
  • Trichoderma asperelloides with salt and alkali tolerance and application of Trichoderma asperelloides
  • Trichoderma asperelloides with salt and alkali tolerance and application of Trichoderma asperelloides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation and identification of Trichoderma aculeatus TUCIM 10320

[0029] Trichoderma aculeatus TUCIM 10320 was isolated from the rhizosphere soil of reeds in the beach area of ​​Dafeng Port, Jiangsu Province. The strain could cover a plate with a diameter of 9 cm when cultured on PDA medium at 28°C for 72 hours, and the aerial hyphae were white and fuzzy. , producing a large number of dark green conidia on the front of the colony. After morphological identification and molecular identification based on ITS, rpb2, and tef1 gene fragments, the strain was Trichoderma asperelloides (Trichoderma asperelloides), which was finally named Trichoderma asperelloides TUCIM 10320, see the evolutionary tree figure 1 .

[0030] The Trichoderma aculeatus is screened in coastal saline soil, which is friendly to the local environment; it produces a large amount of spores, which is convenient for agricultural production and utilization, and the indigenous Trichoderma has str...

Embodiment 2

[0032] Embodiment 2: the saline-alkali tolerance of Trichoderma aculeatus TUCIM 10320

[0033] Phragmites reed can grow normally in coastal saline soil with a salt content of 1.5% and pH = 8.5, so Trichoderma isolated from the rhizosphere soil samples of Phragmites is highly likely to tolerate high-salt and high-alkaline environments. From the Trichoderma screened, select the strains with fast growth and many spores to carry out NaCl and pH tolerance test as follows:

[0034] 2.1 Salt tolerance test:

[0035] Trichoderma aculeatus TUCIM 10320 strain was used as the main research object, and two other fast-growing, sporogenous Trichoderma strains were selected for salt tolerance test. Inoculate the three bacteria on the PDA plate at the same time, culture at 28°C for 7 days, after producing a large number of conidia, scrape the spores under sterile conditions and dilute them to OD with sterile water 600 = 0.1. First, distribute PDB medium (potato dextrose medium, without aga...

Embodiment 3

[0040] Example 3: Inhibitory effect of Trichoderma aculeatus TUCIM 10320 on various plant pathogenic fungi

[0041] Five plant pathogenic fungi were selected, including Pestalotiopsis fici TUCIM 5788, Rhizoctonia solani TUCIM 3753, Fusarium oxysporum TUCIM4848, Alternaria alternata TUCIM 10233), large stem point mold (Macrophomasp.TUCIM 10254), above-mentioned phytopathogenic bacterium is inoculated on one side of the PDA plate of 9cm respectively with the sterilized puncher of above-mentioned phytopathogenic bacterium with the bacterium block of the size of about 5mm in diameter, after 24h, inoculate Trichoderma aculeatus TUCIM 10320 was cultured on the other side of the plate in the dark at 25°C for 14 days, and the fungal confrontation was recorded by taking pictures.

[0042] The results show( image 3 ), Trichoderma aculeatus TUCIM 10320 can inhibit the growth of Phomama, Alternaria, and Rhizoctonia solani colonies, limiting the colony radius to 1-1.5cm, and completely c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Trichoderma asperelloides with salt and alkali tolerance and application of Trichoderma asperelloides. Trichoderma asperelloides TUCIM 10320 is collected in CGMCC on April 3, 2020, with the collection number of CGMCC 19612. The Trichoderma asperelloides TUCIM 10320 can salt stress caused by 1.5 M (8%) salt concentration and alkali stress with pH reaching 9.0 and has bettersalt and alkali tolerance. Under different salt concentration stress, the Trichoderma asperelloides TUCIM 10320 eliminates effects of salt on plant growth inhibiting functions, compared with strain-free control, the crop biomass is increased by 30% and 62% under the condition that the content of NaCl is 0.5% and 0.75% through inoculation of TUCIM 10320, and elongation of crop root systems and differentiation of lateral roots are promoted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a saline-alkali-tolerant Trichoderma aculeatus and its application. Background technique [0002] There are about 1.5 billion mu of saline soil in my country, which is the main reserve land resource in our country. With the acceleration of its natural desalination and artificial improvement, the agricultural utilization of saline soil has become an important part of my country's agricultural technology and production. However, due to the low content of organic matter in saline soil, poor physical and chemical properties, few microbial groups, many anions and cations harmful to crops, and high osmotic pressure of soil solution, it is difficult for crops to take root and stand up. Therefore, how to promote the growth of plant roots in saline soil is the key technology. . [0003] Trichoderma spp. belongs to Ascomycota (Ascomycota) Hypocreales, is a kind of ubiquitous and easy to isolate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCC12N1/145C12R2001/885
Inventor 丁明月蔡枫陈巍伊瑞娜·杜鲁兹尼娜
Owner NANJING AGRICULTURAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com