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Large-scale culture method of porcine Japanese encephalitis vaccine antigens

A technology for porcine Japanese encephalitis and vaccine antigens, applied in vaccines, veterinary vaccines, virus antigen components, etc., can solve the problem of high cost and achieve the effect of increasing virus production

Active Publication Date: 2020-07-10
SICHUAN ANIMAL SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional culture method to cultivate JEV virus can only harvest the virus once, and the cost is high

Method used

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  • Large-scale culture method of porcine Japanese encephalitis vaccine antigens
  • Large-scale culture method of porcine Japanese encephalitis vaccine antigens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The establishment of embodiment 1, JEV culture technique

[0028] 1. Determination of the optimal inoculation volume

[0029] Method 1: (1) Inoculate PK-15 cells in a 10cm culture dish, add DMEM medium containing 10% (volume percentage) calf serum, place at 37°C, 5% CO 2 Culture in the incubator until the cells form a dense monolayer (the number of cells is about 5×10 6 (2) after completing step (1), select a 10cm culture dish with good cell growth, and discard the cell culture medium; (3) after completing step (2), add porcine type B in the amount of 1ml virus solution / dish Encephalitis virus solution (the virus solution is obtained by diluting the virus stock solution with DMEM culture fluid without calf serum), the amount of virus inoculated is 2.5×10 5.8 ~8×10 6.8 TCID 50 (about 0.4 ~ 10MOI); after inoculation, place at 37°C, 5% CO 2 Adsorb in the incubator for 1 h; (4) After completing step (3), discard the virus liquid, add 10 ml of DMEM medium containing 2% ...

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Abstract

The invention discloses a large-scale culture method of porcine Japanese encephalitis vaccine antigens. According to the large-scale culture process of the porcine Japanese encephalitis vaccine antigens, porcine kidney cells PK-15 are used for culturing porcine Japanese encephalitis virus, multiple batches of virus can be continuously harvested through one-time virus inoculation, and the titer ofthe virus is equivalent to that of BHK-21 and VERO cells or higher than an existing culture process, the yield of the virus is greatly increased, the production cost is reduced, and the method is a porcine Japanese encephalitis vaccine antigen culture process suitable for large-scale production.

Description

technical field [0001] The invention relates to the field of biological vaccines, in particular to a large-scale cultivation method for porcine Japanese encephalitis vaccine antigens. Background technique [0002] Japanese encephalitis virus (JEV) can cause Japanese encephalitis disease. Japanese encephalitis is a zoonotic disease transmitted by mosquitoes, and it is an acute disease of the central nervous system that seriously threatens the health of humans and animals. infectious disease. Pig is an important storage host and amplification host of porcine Japanese encephalitis virus, and is the main source of infection of Japanese encephalitis. Porcine Japanese encephalitis virus is one of the main pathogens causing reproductive disorders in sows, and it is mainly prevented by immunization at present. Porcine Japanese encephalitis virus can proliferate in a variety of tissue culture cells, among which BHK-21, Vero and C6 / 36 cells are more sensitive and can be used for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
CPCA61K39/12A61K2039/552A61P31/14C12N7/00C12N2770/24134C12N2770/24151Y02A50/30
Inventor 周远成邝声耀阴文奇
Owner SICHUAN ANIMAL SCI ACAD
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