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30 results about "Porcine kidney" patented technology
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Porcine Kidney. Porcine kidneys have been invaluable to researchers investigating renal issues in human patients. For example, porcine renal tissue has been useful in ultrasound elastography, a way of determining the graft health of a transplanted kidney.
Method for preparing double yolk antibody of porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus
InactiveCN101845095AStrong specificityStrong targetingEgg immunoglobulinsImmunoglobulins against virusesPig farmsYolk
The invention discloses a method for preparing a double yolk antibody of a porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus. The method comprises the following steps of: performing porcine transmissible gastroenteritis virus multiplication on porcine kidney cells (PK15); performing porcine epidemic diarrhea virus multiplication on African green monkey kidney cells (Vero); emulsifying the two cell cultures used as antigen with an oil emulsion adjuvant to prepare immunogen, namely, mixing the two kinds of viruses in a ratio of (1-3):(1-3) to prepare the immunogen; immunizing non-immunologic laying hens; and obtaining the double yolk antibody which can prevent and treat porcine transmissible gastroenteritis and porcine epidemic diarrhea based on the collection and purification of the yolk. When the double yolk antibody is used for curing experimental pigs, the clinical symptoms in the experiment are obviously reduced compared with a control group, and the death rate of the experimental group is obviously lower than that of the control group. The double yolk antibody has obvious preventing and treating functions when applied in a pig farm with high incidence rate of the porcine transmissible gastroenteritis and the porcine epidemic diarrhea.
Owner:PU LIKE BIO ENG
Large-scale full-suspension culture method of porcine circovirus type 2
ActiveCN107312746AReduce dosageImprove immunityViral antigen ingredientsMicroorganism based processesEngineeringPorcine kidney
The invention discloses a large-scale full-suspension production method of a porcine circovirus type 2. The inventors of the invention domesticate a porcine kidney cell adaptable to large-scale serum-free full-suspension culture, which is named as sPK15-YP, is collected in the China General Microbiological Culture Collection Center and has the culture collection number of CGMCC NO.13846. The sPK15-YP cell adaptable to full-suspension culture is used for achieving serum-free large-scale culture of the porcine circovirus type 2 (PCV2), so that the conventional spinner bottle culture technology is replaced, the human resource is reduced, the product quality is improved, and the bottleneck that the virus content is low during PCV2 full-virus culture is solved; by a full-suspension sPK15-YP cell technology, a high-potency PCV2 semifinished product is prepared; by a hollow fiber method, a PCV2 virus culture solution is concentrated and purified to obtain a more pure PCV2 virus concentrated antigen. By the large-scale full-suspension production method, a solid foundation is laid for studying a PCV2 multivalent vaccine, the dosage of the vaccine is reduced, the stress of a swine herd is reduced and the immune level of the swine herd is enhanced.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Culturing method of porcine circovirus type 2-containing cells
InactiveCN104232563ASimple processStable proliferationArtificial cell constructsVertebrate cellsMicroorganismBiological property
The invention relates to a culturing method of porcine circovirus type 2-containing cells. The method comprises the following steps: cloning of an individual porcine circovirus type 2-containing PK15 cell and culturing of a subgroup of the PK15 cell: inoculating PCV2 / SN07-12 seed virus into porcine circovirus type 1 pollution-free porcine kidney cells (PK15 cells), screening positive porcine circovirus type 2-containing PK15 cell cloning strains, and multiplying high-titer porcine circovirus type 2 to achieve virus titer up to 10<7.0>TCID50 / ml, wherein the microbial collection registration number of the PCV2 / SN07-12 seed virus is CCTCC NO:V201304. Compared with the prior art, the cells cultured by adopting the method have the advantages of being simple in technology, high in virus titer, stable in biological characteristics, and the like.
Owner:上海佳牧生物制品有限公司 +2
Continuous porcine kidney cell line constitutively expressing bovine αVβ6 integrin with increased susceptibility to foot and mouth disease virus
ActiveUS9121010B1Promote growthAccurate and Rapid DiagnosisSsRNA viruses negative-senseSsRNA viruses positive-senseDiseaseVaccination
Foot and out disease virus (FMDV) is worldwide problem. Rapid isolation, serotyping and vaccine matching of FMDV from infected animals is critical to enable the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Current virus isolation protocols use primary cells, known to be susceptible to FMDV, or baby hamster kidney cells (BHK-21) and other cell lines that are not highly sensitive to some strains of FMDV. The αVβ6 integrin is a principal receptor for FMDV. We therefore transduced the porcine kidney cell line, LFBK, to stably express both the αV and β6 bovine integrin subunits. The LFBK-αVβ6 cell line showed both β6 expression and enhanced susceptibility to FMDV infection for at least 100 cell passages. LFBK-αVβ6 cells are highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophillic strain O / TAW / 97 and are thus a sensitive tool for FMDV isolation.
Owner:UNITED STATES OF AMERICA
Porcine circovirus type 3 strain, and preparation method and application thereof
InactiveCN109251897AStrong pathogenicityClear symptoms of diseaseViral antigen ingredientsAntiviralsDiseasePorcine kidney
The invention provides a porcine circovirus type 3 strain PCV3-LY and application thereof. The virus is the porcine circovirus type 3 rescued by infectious cloning technology at home and abroad for the first time. After the virus is inoculated to monolayer porcine kidney cells PK15 for adherent culture, D-glucosamine treatment is carried out, then culture is continued, a cell culture is harvestedand used as an inoculum of a next round of passage, and finally a cell culture-adapted strain is obtained by successive passages. During the adaptation process, significant cytopathic changes can be observed after 15 passages. Indirect immunofluorescence confirms that the cell is able to support efficient replication of porcine circovirus type 3. The titer of the virus is lgTCID[50]=10<6.53> / mL. An animal regression test finds that two pigs are killed after five 28-day-old pigs are challenged with the virus, and the virus is more pathogenic to piglets. The virus is the porcine circovirus type3 rescued by infectious cloning technology at home and abroad for the first time, and provides direct support for further research on pathogenesis of PCV3 and development of vaccine research for effectively preventing and controlling the disease.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Npro207shRNA for inhibiting classical swine fever virus replication and preparation method thereof
InactiveCN103667298AIncrease flexibilityFermentationPlant genotype modificationPorcine kidneyIn vivo
The invention relates to Npro207shRNA for inhibiting classical swine fever virus replication, which comprises an siRNA sequence. By establishing an shRNA lentiviral vector, replication-defective lentiviruses are obtained, PK-15 cells ( porcine kidney cells ) are infected by the obtained lentiviruses, and an authentication method of a classical swine fever virus replication inhibiting effect is conducted. The sequence has an effect of obviously inhibiting the replication of classical swine fever viruses on sensitive cells. The RNA interference to the in vivo and vitro replication of the classical swine fever viruses is explored, a lentiviral vector-mediated stably integrated RNA interference technology of a specific conserved gene segment targeted to a classical swine fever virus genome is established, and a transgenic animal with siRNA targeted to the classical swine fever viruses is expected to be established. The necessary experimental data is accumulated for the gene function research of shRNA applied to the classical swine fever viruses and the prevention of classical swine fever, and the early preparation for breeding for disease resistance of the animals is provided.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Novel PCV (porcine circovirus) and cell strain co-culturing with novel porcine circovirus
ActiveCN106754747AHigh viral titerReduce investmentArtificial cell constructsUrinary tract/kidney cellsCircovirusApoptosis
The invention discloses a novel PCV (porcine circovirus) mutant strain and a porcine kidney cell strain co-culturing with the novel PCV mutant strain. Cells infected by the novel PCV mutant strain have visible minimal lesions, but cell death is not caused. The sieved porcine kidney cell strain carries a PCV2, so that stable passage can be realized, a large number of viruses are generated, and the prepared viruses can be used for preventing and treating the PCV.
Owner:WUHAN ENG SCI & TECH RESINST +1
PMKRN1 (porcine Makorin ring finger protein 1) gene-knockout porcine somatic cell, method for preparing same and application of pMKRN1 gene-knockout porcine somatic cell
The invention provides a pMKRN1 (porcine Makorin ring finger protein 1) gene-knockout porcine somatic cell, a method for preparing the same and application of the pMKRN1 gene-knockout porcine somaticcell. The pMKRN1 gene-knockout porcine somatic cell, the method and the application have the advantages that mutant PK-15 (porcine kidney-15) cells can be obtained by the aid of gene knockout technologies, high-titer PCV2 (porcine circoviruses type 2) can be quickly replicated and obtained by the aid of the mutant PK-15 cells, and accordingly efficient virus amplification cells can be provided toresearch on PCV2 pathogenic mechanisms and preparation of PCV2 inactivated vaccine and attenuated vaccine.
Owner:NORTHWEST A & F UNIV
CD163 mutant and application thereof
ActiveCN110105443AAvoid infectionMechanism of action of enrichment infectionCell receptors/surface-antigens/surface-determinantsPeptide/protein ingredientsAnti virusWild type
The invention discloses a CD163 mutant and application thereof. According to the CD163 mutant, a 534th site of an amino acid sequence is changed from glutamic acid E to lysine K. By comparing amino acid sequences of CD163 of different species, amino acid at the 534th site is selected for site-directed mutation (the glutamic acid E is mutated into the lysine K, namely E534K), and an eukaryotic expression vector system is constructed; a wild type (WT) and the mutant CD163 are subjected to recombinant expression in a porcine kidney PK-15 cell; the cell is infected with a PRRSV classical strain BJ-4 (GenBank No.AF331831) and an HP-PRRSV strain HN07-1 (GenBank No.KX766378.1); through the identification of a fluorescent quantitative PCR and a virus titer experiment (50% tissue culture infectivedose, TCID50), the mutation of the amino acid at the 534th site has a significant inhibitory effect on PRRSV-infected host cells, and the CD163 mutant can be applied to breeding of anti-PRRSV pigs andresearch and development of anti-virus vaccines and drugs.
Owner:HENAN ACAD OF AGRI SCI
Method for preparing porcine kidney epithelial cell line for stably expressing BE4 protein based on piggyBac transposon system
InactiveCN112280802AGood experimental basisAids in the study of biological functionsGenetically modified cellsMicroorganism based processesPiggyBac Transposon SystemPorcine kidney
The invention discloses a method for preparing a porcine kidney epithelial cell line for stably expressing BE4 protein, which comprises the following steps: by using porcine kidney epithelial cells (PK15) with good passage as host cells, co-transfecting a piggyBac transposon (PB-BE4max) vector containing a BE4 fusion protein sequence and a transposase expression vector plasmid, screening a positive cell population by puromycin, and selecting a monoclonal cell line; and verifying the editing activity amplifying the characteristic fragment of BE4 and transfecting active sgRNA, so as to obtain the transformant for stably expressing BE4 protein. The porcine kidney epithelial cell line prepared by the method can stably express BE4 protein, and has important application value in the porcine genetic engineering fields of porcine genome function research, transgenic pig preparation, drug-resistant target screening and the like.
Owner:HUAZHONG AGRI UNIV
Multifunctional enzyme gene HG27 and application
The invention discloses multifunctional enzyme gene HG27 and application. The multifunctional enzyme gene HG27 comprises the following steps of comparing a nucleic acid sequence spliced by rhizomys sinensis intestinal microbe macro transcriptome with a glucoside hydrolase family, screening a sequence with relatively high homology, removing original signal peptide, adding pig parotid gland secretory protein signal peptide, optimizing a pig codon, and connecting to a pcd3.1 vector; transfecting to porcine kidney epithelial cells for expression verification, collecting a cell culture solution for enzyme activity analysis, comparing the enzyme activity, selecting the optimal HG27 gene, and detecting the optimal pH value, protein tolerance and enzymatic reaction of different substrates of the HG27 gene, thereby obtaining the multifunctional enzyme gene HG27 which can be applied to the fields of livestock and poultry feed enzyme preparations, transgenic animal preparation, food and medicine industries and the like, and the problems that cellulose is difficult to degrade and absorb and excrement pollutes the environment are solved.
Owner:WENS FOODSTUFF GRP CO LTD +1
A method for improving the impact resistance of anaerobic ammonia oxidation particles
ActiveCN109761348BImprove the ability to resist matrix impactImprove stabilityTreatment with anaerobic digestion processesMedicinePorcine kidney
The invention discloses a method for improving the matrix impact resistance of anaerobic ammonium oxidation particles, which belongs to the field of wastewater treatment. During the matrix shocking period of the ANAMMOX reactor in stable operation, 1500-2500 mg / L total nitrogen shock was performed for 12-48 hours, and 0.1 mg / L-1.5 g / L porcine kidney acylase I was added to the reactor every 1 hour until the matrix The impact is over; AHLs inhibitor porcine kidney acylase I will no longer be added to the reactor after the matrix impact is stopped. The present invention suppresses the activity of the quorum sensing system of the ANAMMOX particles under the impact of the matrix by adding a lower concentration of AHLs inhibitor porcine kidney acylase I, thereby reducing the release amount of the granular AHLs to control the excessive release of LB-EPS, and improving the granule under the impact of the matrix. stability and settling capacity. At the same time, when the inhibitor concentration is low, it will not adversely affect the particle activity.
Owner:BEIJING UNIV OF TECH
A sgRNA targeted to knock out the sst gene and its CRISPR/Cas9 system and application
ActiveCN111778252BFast growthGenetically modified cellsMicrobiological testing/measurementFibroblastPorcine kidney
The invention provides a sgRNA targeting knockout SST gene and its CRISPR / Cas9 system and application, belonging to the technical field of gene knockout. The present invention provides a sgRNA targeted to knock out the SST gene, including SST sgRNA1 and SST sgRNA2, and also provides a CRISPR / Cas9 system comprising the sgRNA. The CRISPR / Cas9 system is transfected into porcine kidney fibroblasts, and the positive cells obtained by screening are obtained to obtain a higher gene deletion efficiency. At the same time, the CRISPR / Cas9 system can improve the efficiency of gene deletion by completely knocking out the pig SST gene. The growth rate of pigs, thereby improving the economic benefits of pig farming, and the CRISPR / Cas9 system is of great significance for further research on the function of the SST gene and its correlation with certain diseases.
Owner:武汉纽利特生物技术有限公司
Application of salinomycin in preparation of anti-coronavirus drugs
ActiveCN112843043AImprove bindingSuppressed copy numberOrganic active ingredientsAntiviralsPharmaceutical drugPorcine kidney
The invention discloses application of salinomycin in preparation of an anti-coronavirus drug, and belongs to the technical field of biological medicines. Specifically, the invention discloses application of salinomycin in preparation of the anti-TGEV medicine, and tests prove that salinomycin with the concentration of 0.2-5 mu M has no obvious toxic effect on porcine kidney cells PK-15, salinomycin with the concentration of 5 mu M can significantly inhibit the copy number of TGEV in infection of PK-15, and salinomycin can become a new choice for preparation of the anti-TGEV medicine. The salinomycin is newly used as an old medicine, pharmacokinetic data of salinomycin on the market are detailed, the safety is reliable, second-stage clinical evaluation can be carried out quickly through development of the new application, the research and development period is shortened, the development cost is saved, and resources can be utilized to the maximum extent.
Owner:HUAZHONG AGRI UNIV
Patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity
InactiveCN113209195AInhibition of reproductionEasy to operateMicrobiological testing/measurementAntiviralsPatriniaCulture cell
The invention provides patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity, and belongs to the technical field of preventive veterinary medicine. The patrinia scaniosaefolia is characterized in that the anti-pseudorabies virus medicine raw material capable of effectively inhibiting PRV reproduction in PK-15 cells on in-vitro culture cells PK-15 (pig kidney cells) within 48h is 0.5mg / mL of patrinia scaniosaefolia; when the concentration of the patrinia scaniosaefolia is 500 micrograms / mL, the virus inhibition rate reaches 90 percent; the antiviral activity is dose-dependent; the half inhibition concentration IC50 on PRV is 54.85 micrograms / mL; the treatment index TI of the patrinia scaniosaefolia is 10.557; the inactivation effect of the patrinia scaniosaefolia on the virus is obvious; the virus inhibition rate of the inactivation effect reaches 90 percent; the treatment effect on the virus is weak; and no protection effect is achieved on the cells. The patrinia scaniosaefolia has the advantages that the operation is simple; the use is convenient; the effect is obvious; and the effect that the patrinia scaniosaefolia can resist the pseudorabies virus through in-vitro inactivation of the pseudorabies virus is verified.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Large-scale culture method of porcine Japanese encephalitis vaccine antigens
ActiveCN111394319AIncrease productionSuitable for large-scale productionSsRNA viruses positive-senseViral antigen ingredientsAntigenJapanese encephalitis vaccine
The invention discloses a large-scale culture method of porcine Japanese encephalitis vaccine antigens. According to the large-scale culture process of the porcine Japanese encephalitis vaccine antigens, porcine kidney cells PK-15 are used for culturing porcine Japanese encephalitis virus, multiple batches of virus can be continuously harvested through one-time virus inoculation, and the titer ofthe virus is equivalent to that of BHK-21 and VERO cells or higher than an existing culture process, the yield of the virus is greatly increased, the production cost is reduced, and the method is a porcine Japanese encephalitis vaccine antigen culture process suitable for large-scale production.
Owner:SICHUAN ANIMAL SCI ACAD +1
Method for producing swine fever live vaccine by using porcine kidney cell line (IBRS-2) and product thereof
ActiveCN101850115BGuaranteed to be pureEnsure safetyAntiviralsRecovery/purificationImmune effectsFreeze-drying
Owner:广东永顺生物制药股份有限公司 +1
A kind of cd163 mutant and its application
ActiveCN110105443BAvoid infectionMechanism of action of enrichment infectionCell receptors/surface-antigens/surface-determinantsPeptide/protein ingredientsPig breedingPig kidney
The invention discloses a CD163 mutant and application thereof. The 534th position of the CD163 amino acid sequence is changed from glutamic acid E to lysine K. In the present invention, by comparing the amino acid sequences of CD163 of different species, the 534th amino acid is selected for site-directed mutation (glutamic acid E is mutated into lysine K, namely E534K), and a eukaryotic expression vector system is constructed; in pig kidney PK‑15 cells Recombinantly express wild type (wild type, WT) and mutant CD163; Infect cells with PRRSV classic strain BJ‑4 (GenBank number AF331831) and HP‑PRRSV strain HN07‑1 (GenBank number KX766378.1); by fluorescence Quantitative PCR, virus titer experiment (half cell infection dose, 50% tissue culture infectious dose, TCID 50 ) identification, the 534th amino acid mutation has a significant inhibitory effect on PRRSV infection of host cells, and can be applied to anti-PRRSV pig breeding, anti-virus vaccine and drug development.
Owner:HENAN ACAD OF AGRI SCI
Luteolin with specific in-vitro anti-pseudorabies virus activity
InactiveCN113244215AInhibition of reproductionEasy to operateOrganic active ingredientsMicrobiological testing/measurementPharmaceutical drugPorcine kidney
The invention relates to luteolin with specific in-vitro anti-pseudorabies virus activity, belongs to the technical field of preventive veterinary medicine, and is characterized in that 20 mu g / mL luteolin is used as a raw material of a drug capable of effectively inhibiting the reproduction of PRV in porcine kidney cells PK-15 on the porcine kidney cells PK-15 cultured in vitro within 48 hours; the luteolin has effects on replication, adsorption and penetration stages of virus infection; the inhibition effect on the replication stage is most obvious; when the concentration of the luteolin is 20 mu g / mL, the luteolin has no inactivation effect on viruses and has no protection effect on cells; when the luteolin is added in the virus replication stage, virus replication is inhibited by 10 times; when the concentration of the luteolin is below 20 mu g / mL, the inhibition rate of the luteolin to viruses reaches 80%; the antiviral activity is dose-dependent; the median inhibitory concentration IC50 to PRV is 6.775 mu g / mL; and the therapeutic index of the luteolin is 3.1542.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
A large-scale full suspension culture method of porcine circovirus type 2
ActiveCN107312746BReduce dosageImprove immunityViral antigen ingredientsMicroorganism based processesPorcine kidneyTGE VACCINE
The invention discloses a large-scale full-suspension production method of a porcine circovirus type 2. The inventors of the invention domesticate a porcine kidney cell adaptable to large-scale serum-free full-suspension culture, which is named as sPK15-YP, is collected in the China General Microbiological Culture Collection Center and has the culture collection number of CGMCC NO.13846. The sPK15-YP cell adaptable to full-suspension culture is used for achieving serum-free large-scale culture of the porcine circovirus type 2 (PCV2), so that the conventional spinner bottle culture technology is replaced, the human resource is reduced, the product quality is improved, and the bottleneck that the virus content is low during PCV2 full-virus culture is solved; by a full-suspension sPK15-YP cell technology, a high-potency PCV2 semifinished product is prepared; by a hollow fiber method, a PCV2 virus culture solution is concentrated and purified to obtain a more pure PCV2 virus concentrated antigen. By the large-scale full-suspension production method, a solid foundation is laid for studying a PCV2 multivalent vaccine, the dosage of the vaccine is reduced, the stress of a swine herd is reduced and the immune level of the swine herd is enhanced.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Method for overexpression of porcine circovirus type 2 nucleocapsid protein in cells of mammal
ActiveCN102586326BHigh expressionVector-based foreign material introductionVirus ProteinPorcine kidney
The invention discloses a method for overexpression of porcine circovirus type 2 nucleocapsid protein in cells of a mammal. According to the method disclosed by the invention, the stability is enhanced and the expression level of the virus protein in the cells of the mammal is further improved by inhibiting the ubiquitination of the porcine circovirus type 2 nucleocapsid protein. The method comprises the following steps: 1) cloning the genome sequence of a porcine circovirus type 2 SX04 strain; 2) constructing a eukaryotic expression plasmid containing the porcine circovirus type 2 (PCV2) nucleocapsid protein; 3) performing transfection on porcine kidney cells PK15 through the plasmid, and adding MG132 into a cell culture solution at 24 hours after transfection; and 4) culturing continuously for 48 hours, then collecting the cells and detecting the expression protein by immunoblotting. Compared with an ordinary eukaryotic expression method, the method has the advantage that the PCV2 nucleocapsid protein in the eukaryotic cells can be effectively stabilized. The detection by protein immunoblotting at 72 hours after transfection shows that, through the adoption of the method disclosed by the invention, the expression level of the PCV2 nucleocapsid protein can be improved by about 20 times to the greatest extent.
Owner:杭州贝尔塔生物技术有限公司
Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof
ActiveCN101948536BEasy to produceReduce manufacturing costEgg immunoglobulinsDigestive systemYolkAntigen
Owner:PULIKE BIOLOGICAL ENG INC
New porcine circovirus and its co-cultured cell line
ActiveCN106754747BHigh viral titerObvious visual lesion featuresArtificial cell constructsUrinary tract/kidney cellsCircovirusCulture cell
The invention discloses a novel PCV (porcine circovirus) mutant strain and a porcine kidney cell strain co-culturing with the novel PCV mutant strain. Cells infected by the novel PCV mutant strain have visible minimal lesions, but cell death is not caused. The sieved porcine kidney cell strain carries a PCV2, so that stable passage can be realized, a large number of viruses are generated, and the prepared viruses can be used for preventing and treating the PCV.
Owner:WUHAN ENG SCI & TECH RESINST +1
Compound porcine alpha interferon gene and synthetic method thereof
InactiveCN104878016AGood antiviral activitySuitable for industrial productionFermentationVector-based foreign material introductionPichia pastorisEscherichia coli
The invention discloses a compound porcine alpha interferon gene. Compared with a yeast expressed natural porcine alpha interferon gene, the compound porcine alpha interferon gene has the advantages that the expressed protein of the compound porcine alpha interferon gene is better in antiviral activity, and the function of resisting 100TCID50VSV virus infection on a porcine kidney cell is improved by 50 times. The invention further provides a synthetic method of the compound porcine alpha interferon gene. The synthetic method adopts pichia pastoris X-33 as a template, requires no additional inducer, and is suitable for industrial production. Due to the adoption of pichia pastoris, toxins which are carried by escherichia coli expressed foreign protein and toxic to porcine cells can be avoided, that is, the toxicity is reduced. The synthetic method is suitable for synthesis of the compound porcine alpha interferon gene.
Owner:JIANGSU HFQ BIO TECH CO LTD
Method for producing swine fever live vaccine by using porcine kidney cell line (IBRS-2) and product thereof
ActiveCN101850115AGuaranteed to be pureEnsure safetyAntiviralsRecovery/purificationImmune effectsFreeze-drying
The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (IBRS-2). The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine; (2) carrying out sub-culturing on the cell for producing the vaccine; (3) reproducing virus cell seeds; (4) reproducing venom for producing the vaccine; and (5) preparing the vaccine, and performing split charging and freeze-drying. The method of the invention has the advantages of simple and stable production process, easy operation, high content of virus, small difference between bitches, and controllable quality, and capability of improving the quality and yield of the vaccine. The swine fever live vaccine produced by the method of the invention has high safety and high immune effect, and has a complete immune protection function on the attacks of the strong swine fever viruses.
Owner:广东永顺生物制药股份有限公司 +1
Scale cultivation method of porcine Japanese encephalitis vaccine antigen
ActiveCN111394319BIncrease productionSuitable for large-scale productionSsRNA viruses positive-senseViral antigen ingredientsAntigenVaccination
The invention discloses a large-scale cultivation method of porcine Japanese encephalitis vaccine antigen. The invention provides a large-scale culture process for porcine Japanese encephalitis vaccine antigens, using pig kidney cells PK-15 cells to culture porcine Japanese encephalitis virus, one time of virus inoculation can continuously harvest multiple batches of viruses, and the virus titer It is equivalent to or higher than the existing culture technology of BHK‑21 and VERO cells, greatly improves the virus yield and reduces the production cost, and is a porcine Japanese encephalitis vaccine antigen culture technology suitable for large-scale production.
Owner:SICHUAN ANIMAL SCI ACAD +1
sgRNAs that efficiently edit the porcine miR-17-92 gene cluster
ActiveCN108642055BImprove securityEliminate biological safety hazardsNucleic acid vectorVector-based foreign material introductionBiotechnologyPorcine kidney
Owner:广东明珠生物技术有限公司
Iron deficiency model of porcine kidney cell and establishing method thereof
InactiveCN107119009APrevent Iron Deficiency AnemiaPrevent anemiaCulture processArtificial cell constructsPhysiologyPorcine kidney
The invention discloses an iron deficiency model of a porcine kidney cell and an establishing method thereof. The establishing method comprises the following steps of S1, culturing of the porcine kidney cell; S2, inducing of iron deficiency: adding an iron deficiency inducing agent into a basic culture liquid of the porcine kidney cell, and enabling the porcine kidney cell to produce iron deficiency strain, so as to obtain the iron deficiency model of the porcine kidney cell. The iron deficiency model can provide reliable techniques and methods for deeply researching and preventing iron-deficiency anemia of new animals.
Owner:YUNNAN AGRICULTURAL UNIVERSITY
A kind of porcine somatic cell knocked out of pmkrn1 gene and its preparation method and application
The invention provides a pMKRN1 (porcine Makorin ring finger protein 1) gene-knockout porcine somatic cell, a method for preparing the same and application of the pMKRN1 gene-knockout porcine somaticcell. The pMKRN1 gene-knockout porcine somatic cell, the method and the application have the advantages that mutant PK-15 (porcine kidney-15) cells can be obtained by the aid of gene knockout technologies, high-titer PCV2 (porcine circoviruses type 2) can be quickly replicated and obtained by the aid of the mutant PK-15 cells, and accordingly efficient virus amplification cells can be provided toresearch on PCV2 pathogenic mechanisms and preparation of PCV2 inactivated vaccine and attenuated vaccine.
Owner:NORTHWEST A & F UNIV
Porcine MLKL gene deletion cell strain capable of promoting proliferation of pseudorabies virus and application of porcine MLKL gene deletion cell strain
ActiveCN114457078APromote proliferationHigh culture titerGenetically modified cellsTransferasesRabiesPorcine kidney
The invention discloses a swine MLKL gene deletion cell strain capable of promoting proliferation of pseudorabies virus and application of the swine MLKL gene deletion cell strain, and belongs to the technical field of biology. The invention discloses sgRNA for knocking out a swine MLKL gene, which is characterized in that the sgRNA comprises sgRNA1 and sgRNA2, the primer sequence of the sgRNA1 is as shown in SEQ ID NO: 1-2, the primer sequence of the sgRNA2 is as shown in SEQ ID NO: 1-2, and the primer sequence of the sgRNA2 is as shown in SEQ ID NO: 1-2. The primer sequence of the sgRNA2 is as shown in SEQ ID NO: 3-4. The sgRNA primer and a CRISPR / Cas9 carrier are utilized to construct a knockout porcine MLKL gene, then a limited dilution method is adopted to carry out passage and screening on the obtained porcine kidney epithelial cells with the knockout porcine MLKL gene to obtain a porcine MLKL gene deletion cell strain, and after the porcine kidney epithelial cells with the knockout porcine MLKL gene deletion cell strain is inoculated with pseudorabies virus, the cell strain can continuously promote virus proliferation compared with normal porcine kidney epithelial cells.
Owner:INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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