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Iron deficiency model of porcine kidney cell and establishing method thereof

A pig kidney cell and a technology for establishing a method are applied in the field of pig kidney cell iron deficiency model and its establishment, which can solve the problems of the method and model without pig kidney iron deficiency, and achieve low renal cell inhibition rate, easy operation and establishment. simple method effect

Inactive Publication Date: 2017-09-01
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no method and model for the establishment of porcine kidney iron-deficient cells

Method used

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  • Iron deficiency model of porcine kidney cell and establishing method thereof
  • Iron deficiency model of porcine kidney cell and establishing method thereof
  • Iron deficiency model of porcine kidney cell and establishing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for establishing a pig kidney cell iron deficiency model, comprising the following steps:

[0030] S1 porcine kidney cell culture: select primary porcine kidney cells, use DMEM serum-containing culture medium as the base culture medium, at 36°C, 4% CO 2 Cultivate in an incubator.

[0031] Wherein, the pig kidney cells are kidney cells of newborn piglets. This is because newborn piglets generally have symptoms of severe anemia within 7 days if iron is not supplemented after birth. The kidney is the main site for the synthesis of erythropoietin (EPO), which is closely related to erythropoiesis and hemoglobin synthesis. The kidney cells of newborn piglets were selected to establish the iron deficiency model, which is easy to operate and has good repeatability.

[0032] S2 Iron-deficiency induction: Add an iron-deficiency inducer to the basal culture medium of pig kidney cells to cause iron-deficiency stress in pig kidney cells, and obtain an iron-deficiency mod...

Embodiment 2

[0036] A method for establishing a pig kidney cell iron deficiency model, comprising the following steps:

[0037] S1 porcine kidney cell culture: select primary porcine kidney cells, use DMEM serum-containing culture medium as the base culture medium, at 37°C, 6% CO 2 Cultivate in an incubator.

[0038] S2 iron deficiency induction: collect the logarithmic phase cells of pig kidney cells, adjust the concentration of the cell suspension to 10 4 cells / ml, add 2mL to each well; at 37°C, 6% CO 2 Cultivate in an incubator; add an iron deficiency inducer to incubate until the cell monolayer covers the bottom of the well, wherein the concentration of deferoxamine is 125 μM. Add deferoxamine to the basal culture medium of pig kidney cells and incubate for 48 hours to obtain the iron deficiency model of pig kidney cells.

[0039] The kidney cell inhibition rate of the pig kidney cell iron deficiency model obtained by the invention is 0-15%; the iron content of the pig kidney cell i...

Embodiment 3

[0041] A method for establishing a pig kidney cell iron deficiency model, comprising the following steps:

[0042] S1 porcine kidney cell culture: select primary porcine kidney cells, use DMEM serum-containing culture medium as the base culture medium, at 37°C, 6% CO 2 Cultivate in an incubator.

[0043] S2 iron deficiency induction: collect the logarithmic phase cells of pig kidney cells, adjust the concentration of the cell suspension to 10 4 pcs / ml, add 3ml to each well; at 37℃, 6% CO 2 Cultivate in an incubator; add an iron deficiency inducer to incubate until the cell monolayer covers the bottom of the well, wherein the concentration of deferoxamine is 100 μM. Add deferoxamine to the basal culture medium of pig kidney cells and incubate for 24 hours to obtain the iron deficiency model of pig kidney cells.

[0044] The kidney cell inhibition rate of the pig kidney cell iron deficiency model obtained by the invention is 0-15%; the iron content of the pig kidney cell iron...

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Abstract

The invention discloses an iron deficiency model of a porcine kidney cell and an establishing method thereof. The establishing method comprises the following steps of S1, culturing of the porcine kidney cell; S2, inducing of iron deficiency: adding an iron deficiency inducing agent into a basic culture liquid of the porcine kidney cell, and enabling the porcine kidney cell to produce iron deficiency strain, so as to obtain the iron deficiency model of the porcine kidney cell. The iron deficiency model can provide reliable techniques and methods for deeply researching and preventing iron-deficiency anemia of new animals.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a pig kidney cell iron deficiency model and a method for establishing the same. Background technique [0002] Iron is an essential trace element that organisms require the most and is most likely to cause metabolic disorders. Insufficient iron intake can cause anemia or related dysfunction in animals. Therefore, the establishment of iron deficiency models is crucial to the study of iron deficiency diseases. Some current methods of iron deficiency models are: tail-cutting bloodletting method or feeding with low-iron feed. So far, there is no method and model for the establishment of pig kidney iron-deficient cells. Contents of the invention [0003] The purpose of the present invention is to provide a porcine kidney cell iron deficiency model and its establishment method, so as to provide a reliable iron deficiency model for in-depth exploration and prevention of neonatal an...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0686C12N2500/46
Inventor 李美荃郭荣富张春勇陈克嶙安清聪
Owner YUNNAN AGRICULTURAL UNIVERSITY
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