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Patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity

A technology of pseudorabies virus and Patrinia, which is applied in the direction of antiviral agent, microbial measurement/inspection, medical preparations containing active ingredients, etc., can solve the problem of no anti-pseudorabies virus drugs, etc., and achieve obvious effect and use Convenience and easy operation

Inactive Publication Date: 2021-08-06
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problem that there is no anti-pseudorabies virus drug at present, the present invention provides Patrinia officinalis with specific external anti-pseudorabies virus activity, which is mainly evaluated by in vitro experiments. The activity of anti-pseudorabies virus, the experiment verified whether Patrinia can resist pseudorabies virus by inactivating pseudorabies virus in vitro, aiming to solve the problem that there is no anti-pseudorabies virus drug at present

Method used

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  • Patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity
  • Patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity
  • Patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity

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Embodiment 1

[0050] In the present invention, the Patrinia officinalis with anti-pseudorabies virus activity was purchased in February 2021 from Hongteng Medicine Store, Harbin City, Heilongjiang Province, China; the adopted Patrinia virus TJ strain (PRV) came from Harbin Veterinary Medicine, China. Research Institute; the composition of the cell growth medium used is: 89% DMEM medium, 10% fetal bovine serum and 1% double antibody are prepared in a sterile environment and stored in a refrigerator at 4°C; the composition of the cell maintenance medium used It is: 97% DMEM medium, 2% fetal calf serum and 1% double antibody are prepared in a sterile environment and stored in a refrigerator at 4°C; the PRV dilution used is based on the half tissue infection dose (TCID) of the virus stock solution 50 ) and the specific requirements of the experiment were diluted with cell maintenance solution; the instruments used: cell incubator (Thermo), ultra-clean workbench (Suzhou Purification), microplate ...

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Abstract

The invention provides patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity, and belongs to the technical field of preventive veterinary medicine. The patrinia scaniosaefolia is characterized in that the anti-pseudorabies virus medicine raw material capable of effectively inhibiting PRV reproduction in PK-15 cells on in-vitro culture cells PK-15 (pig kidney cells) within 48h is 0.5mg / mL of patrinia scaniosaefolia; when the concentration of the patrinia scaniosaefolia is 500 micrograms / mL, the virus inhibition rate reaches 90 percent; the antiviral activity is dose-dependent; the half inhibition concentration IC50 on PRV is 54.85 micrograms / mL; the treatment index TI of the patrinia scaniosaefolia is 10.557; the inactivation effect of the patrinia scaniosaefolia on the virus is obvious; the virus inhibition rate of the inactivation effect reaches 90 percent; the treatment effect on the virus is weak; and no protection effect is achieved on the cells. The patrinia scaniosaefolia has the advantages that the operation is simple; the use is convenient; the effect is obvious; and the effect that the patrinia scaniosaefolia can resist the pseudorabies virus through in-vitro inactivation of the pseudorabies virus is verified.

Description

technical field [0001] The invention relates to Patrinia officinalis with specific anti-pseudorabies virus activity and belongs to the technical field of preventive veterinary medicine. Background technique [0002] Patrinia (Latin scientific name: Thlaspiarvense Linn., alias: Thlaspiarvense Linn.) is a rooted whole plant of the genus Patrinaceae. It has no other peculiar smell when it is fresh, but it has a strong smell of stinky sauce after drying, so it is called Patrinia. Patrinia is overgrown, with yellow flowers and purple roots, like Bupleurum, like celery flowers and cnidium flowers; Its root is white and purple, resembling Bupleuri, slightly bitter and sweet. Patrinia has the advantages of rich types of active ingredients, wide application, wide distribution, rich resources, etc. It is a wild plant resource with high development and utilization value and medicinal value. According to textual research, the earliest recorded Paijiang in ancient Chinese herbal medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/84A61P31/22
CPCA61K36/84A61P31/22C12Q1/70
Inventor 朱妍门晓钰蔡晓静
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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