Patrinia scaniosaefolia with in-vitro anti-pseudorabies virus activity
A technology of pseudorabies virus and Patrinia, which is applied in the direction of antiviral agent, microbial measurement/inspection, medical preparations containing active ingredients, etc., can solve the problem of no anti-pseudorabies virus drugs, etc., and achieve obvious effect and use Convenience and easy operation
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[0050] In the present invention, the Patrinia officinalis with anti-pseudorabies virus activity was purchased in February 2021 from Hongteng Medicine Store, Harbin City, Heilongjiang Province, China; the adopted Patrinia virus TJ strain (PRV) came from Harbin Veterinary Medicine, China. Research Institute; the composition of the cell growth medium used is: 89% DMEM medium, 10% fetal bovine serum and 1% double antibody are prepared in a sterile environment and stored in a refrigerator at 4°C; the composition of the cell maintenance medium used It is: 97% DMEM medium, 2% fetal calf serum and 1% double antibody are prepared in a sterile environment and stored in a refrigerator at 4°C; the PRV dilution used is based on the half tissue infection dose (TCID) of the virus stock solution 50 ) and the specific requirements of the experiment were diluted with cell maintenance solution; the instruments used: cell incubator (Thermo), ultra-clean workbench (Suzhou Purification), microplate ...
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