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Application of FGF21 in preparation of medicine for treating colorectal cancer

A FGF21, colorectal cancer technology, applied in the field of medicine, can solve the problems of many side effects and weak targeting, and achieve the effects of alleviating the occurrence and development, inhibiting the generation and metastasis, and reducing the size

Active Publication Date: 2020-07-17
无锡代达康健生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently developed drugs are all small molecule inhibitors with weak targeting and more side effects, while bioprotein drugs absorb quickly and have strong targeting

Method used

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  • Application of FGF21 in preparation of medicine for treating colorectal cancer
  • Application of FGF21 in preparation of medicine for treating colorectal cancer
  • Application of FGF21 in preparation of medicine for treating colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Application of FGF21 in the preparation of medicines

[0030] The FGF21 protein is prepared by culturing microbial cells, and the preparation method has been disclosed in the patent application with the authorized announcement number CN106432509B.

[0031] The obtained FGF21 protein prepared by microbial cells is used together with a pharmaceutically acceptable carrier or adjuvant to prepare a drug, and the pharmaceutically acceptable carrier or adjuvant can be an excipient, adjuvant and / or diluent, etc.

Embodiment 2

[0032] Example 2 The effect of FGF21 on the growth rate of human colorectal cancer xenografts

[0033] The FGF21 protein whose amino acid sequence is shown in SEQ ID NO.1 was prepared according to the method in Example 1.

[0034] Human colon cancer cell SW620 cells were divided into 1 × 10 6 cells / only inoculated subcutaneously in 6-week-old male nude mice until the tumor grows to 200mm 3 They were randomly divided into four groups. ①Normal saline group: injected with equal volume of normal saline; ②Low-dose group: injected with 1 mg / kg FGF21; ③Medium-dose group: injected with 5 mg / kg FGF21; ④High-dose group: injected with 10 mg / kg FGF21. Inject once a day for 15 consecutive days. Tumor volume was monitored daily, mice were sacrificed three weeks later, and tumor weight was measured. The results showed that three different doses of FGF21 could inhibit the transplanted tumor volume and final tumor weight in a dose-dependent manner. After high-dose FGF21 (10mg / kg) injection...

Embodiment 3

[0035] Example 3 Effect of FGF21 on AOM / DSS-induced colorectal cancer in mice

[0036] The FGF21 protein whose amino acid sequence is shown in SEQ ID NO.1 was prepared according to the method in Example 1.

[0037] Take C57 mice and inject azoxymethane 10mg / kg in the first week, and start a 2.5% dextran sodium sulfate free consumption cycle at the same time, each cycle includes three weeks and 21 days, and drink dextran sodium sulfate in the first week Drink ordinary water for the next two weeks, with three weeks as a cycle, a total of three cycles. From the sixth week, they were divided into normal saline group and FGF21 injection group. The injection dose of FGF21 injection group was 10 mg / kg, and the normal saline group was injected with the same amount of normal saline once a day for 4 consecutive weeks.

[0038] The result is as figure 2 As shown in A, the average number of tumor foci can be reduced from 4 to 1 after injection of FGF21, figure 2 B shows the typical p...

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Abstract

The invention discloses an application of FGF21 in preparation of a medicine for treating colorectal cancer, and belongs to the technical field of medicines. The FGF21 protein provided by the invention is a secretory protein composed of about 210 amino acids. The invention finds for the first time that the FGF21 has the effect of treating colorectal tumor, and cytological experiments and animal experiments prove that the FGF21 can inhibit proliferation, invasion and migration of a colorectal tumor cell line, and can obviously reduce the size of a tumor focus in a mouse model. The medicine which is used for treating colorectal cancer and is prepared by taking the FGF21 as an active substance has the effects of being good in safety, long in medicine effect lasting time, capable of protectingorgans from being damaged and capable of improving lipid metabolism disorder.

Description

technical field [0001] The invention relates to the application of FGF21 in the preparation of medicines for treating colorectal cancer, in particular to the application of human FGF21 in the preparation of medicines for treating colorectal cancer, belonging to the technical field of medicine. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in clinical practice. Colorectal cancer includes colon cancer and rectal cancer, and it is the third most common malignant tumor in my country. The incidence of colorectal cancer is on the rise with the improvement of living standards. Worldwide, colorectal cancer ranks third in the incidence of common malignant tumors with 1,023,152 new cases per year, accounting for 10.38% of the total incidence of malignant tumors. In my country, with the change of residents' lifestyle and dietary structure, the incidence of colorectal cancer is increasing at an annual rate of 4%, and the average annual growth...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P1/00A61P35/00
CPCA61K38/1825A61P1/00A61P35/00A61P3/08A61P3/10A61P3/00G01N33/57419
Inventor 朱升龙陈永泉叶贤龙
Owner 无锡代达康健生物医药科技有限公司
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