Preparation and use method of low-temperature cellulase

A low-temperature cellulase and cellulase technology, applied in the biological field, can solve the problems of low fermentation temperature, environmental secondary pollution, singleness, etc., and achieve the effects of low growth temperature, no secondary pollution, and low cost

Pending Publication Date: 2020-07-17
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to aim at the comparatively single way that people utilize cellulose at the present stage, mainly divided into two aspects of physical and chemical utilization and biological utilization, physical and chemical methods such as strong acid (alkali), high temperature, ion radiation can degrade cellulose, but this The extreme environment is not easy to control, and it will cause secondary pollution to the environment. A method for the preparation and use of low-temperature cellulase is proposed. Cellulase is produced by low-temperature fermentation of cold-tolerant Brevibacterium, which has a good cellulose degradation effect. Low temperature and no secondary pollution, can reduce the temperature in the fermentation process and application process, reduce energy consumption, and produce better economic benefits

Method used

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  • Preparation and use method of low-temperature cellulase
  • Preparation and use method of low-temperature cellulase
  • Preparation and use method of low-temperature cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Detection of the cellulase activity of the low temperature cellulase

[0046] In order to explore the production of cellulase produced by the strain under the current conditions, insert Bacillus cereus into the culture medium under the above conditions to ferment and produce enzymes according to the above conditions, and do three parallel groups. After the fermentation, take fresh fermentation broth at 4°C Centrifuge at 10000r / min for 10min, and the supernatant obtained by centrifugation is the low-temperature cellulase enzyme solution of the strain; the enzyme activity of the cellulase in the enzyme solution is measured by 3,5-dinitrosalicylic acid colorimetry, and the enzyme activity is expressed as 1mL of cellulase solution catalyzes the substrate to generate 1.0μg of glucose in 1min, which is an enzyme activity unit. The measurement results are shown in Table (2).

[0047] Table 2)

[0048]

[0049] As can be seen from the measurement results in Table (2), when...

Embodiment 2

[0051] Determination of Enzymatic Properties of Low Temperature Cellulase

[0052] The enzymatic activity of the obtained cellulase liquid is measured, and the stability of the cellulase liquid to temperature and pH is measured respectively, so as to determine the application conditions of the cellulase. Place the crude enzyme solution obtained by centrifugation at 10°C, 20°C, 30°C, 40°C, 45°C, 50°C, 60°C, 70°C, 80°C, and 90°C and incubate for 60 minutes, and measure the enzyme activity after rapid cooling. The untreated crude enzyme is 100% and calculates the remaining enzyme activity percentage, the result is as follows figure 1 shown. Adjust the crude enzyme solution obtained by centrifugation to different pH (3-8), incubate at 4°C for 60 minutes, calculate the percentage of remaining enzyme activity with the untreated crude enzyme as 100%, and the result is as follows figure 2 shown.

[0053] like figure 1 As shown, the cellulase activity is measured after the crude e...

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Abstract

The invention relates to a preparation and use method of low-temperature cellulase, and belongs to the technical field of biology. The principle of the preparation and use method of cellulase is clear, an enzyme-producing strain used in the invention is brevibacterium frigoritolerans, and the strain has the advantages of fast growth and propagation speed, low growth temperature, good enzyme-producing performance and the like; and the cellulase is simple in preparation process, relatively low in cost, good in acid resistance, stable in performance at low temperature and good in enzyme activity.The method is free from secondary pollution to environment, the crop straws can be degraded through the cellulase, required energy substances can be obtained, the temperature required by the reactionis low, energy loss is low, higher economic value can be generated, higher economic returns can be obtained at low cost, and the method has good performance in the aspects of industrial production and agricultural resource waste utilization and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for preparing and using cellulase, in particular to a method for preparing and using low-temperature cellulase. Background technique [0002] Cellulose exists widely in nature and is a series of useful chemicals that can decompose cellulose into glucose and so on. Cellulase decomposing cellulose is a way of using cellulose in nature that is found by current research. The use of cellulose by microorganisms as a nutrient source requires the participation of cellulase. Cellulase is a collective name for a multi-component enzyme system. , can be divided into three types of enzyme components, namely endo-1,4-β-D-glucanase, exo-1,4-β-D-glucanase and β-glucosidase. Under the action of cellulase, cellulose is finally degraded into glucose, which can be further converted into ethanol, butanol, methane and other useful chemicals. [0003] At present, it is generally believed that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N1/20C12P19/14C12P19/02C12R1/13
CPCC12N9/2437C12N1/20C12P19/14C12P19/02C13K1/02
Inventor 王明杨生妹顾明慧孙晓凤王瑶于明浩
Owner YANGZHOU UNIV
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