Biomarker for gastric cancer diagnosis and prognosis evaluation, application of biomarker and detection kit

A biomarker and prognosis assessment technology, applied in the field of biomedicine, can solve problems such as passive and unfavorable patient condition control, achieve high clinical application value, facilitate the prediction of gastric cancer prognosis, and save medical costs.

Pending Publication Date: 2020-07-17
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main disadvantage of the prior art: clinically, the method of judging the prognosis of gastric cancer patients mostly uses postoperative regular

Method used

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  • Biomarker for gastric cancer diagnosis and prognosis evaluation, application of biomarker and detection kit
  • Biomarker for gastric cancer diagnosis and prognosis evaluation, application of biomarker and detection kit
  • Biomarker for gastric cancer diagnosis and prognosis evaluation, application of biomarker and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Detection of expression levels in collected human gastric cancer tissues and adjacent normal tissues by qPCR

[0038] 1. RNA extraction

[0039] 1) Grinding of gastric cancer tissue and paracancerous tissue

[0040] Clean the mortar, pestle, scissors, scalpel, tweezers, and spoons required for grinding with ultrapure water, bake in an oven at 60°C for 3 hours, wrap them in foil paper, and bake in an oven at 180°C overnight; Before tissue grinding, the above-mentioned equipment needs to be pre-cooled by liquid nitrogen; take out the clinical samples stored in liquid nitrogen, cut out soybean-sized tissue pieces and put them in a pre-cooled mortar for grinding, and at the same time, replenish them in small amounts and multiple times. Liquid nitrogen, keep the mortar at ultra-low temperature until it is ground into powder, this process generally takes 10min-15min; after the grinding is complete, add 1mL pre-cooled TRIzoI to continue grinding, and mix the tissue...

Embodiment 2

[0073] Example 2. Tissue microarray immunohistochemical detection of expression levels in gastric cancer tissues and adjacent normal tissues:

[0074] 1. Frozen sections of gastric cancer tissue and paracancerous tissue

[0075] Mark the OCT embedding agent (opti-mum cutting temperature compound) on the fixed head, and then take a tissue of appropriate size (about 24×24×3mm, without embedding) and place it on the OCT. The surface of the tissue is exposed outside the OCT. All tissues were embedded with OCT. Put the fixed head on the quick-cooling table in the freezer, and freeze at -25°C for 10 minutes (the time varies slightly depending on the tissue, and the time for larger tissues or adipose tissue can be longer, generally about 10 minutes). Clamp the frozen head to the microtome, fix the angle of the high-speed knife, use fine adjustment to flatten the tissue to expose the maximum cut surface, adjust the fine adjustment scale to 5 μm, put down the anti-roll plate and start...

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Abstract

The invention relates to a biomarker for gastric cancer diagnosis and treatment and prognosis evaluation, an application of the biomarker and a detection kit. Tumor infiltration immune cytokine IL23Ais used as the biomarker for the gastric cancer diagnosis and treatment and prognosis evaluation, and the relative expression level of the tumor infiltration immune cytokine IL23A is detected to evaluate the prognosis condition of patients. Compared with the prior art, the biomarker provided by the invention has the following advantages: cytokine of the biomarker participates in a plurality of immune response infiltrated by tumor immune cells, can realize the staged development stage of the risk of gastric cancer from a molecular level, rapidly and accurately detect and evaluate the prognosisof the gastric cancer, and effectively improve the sensitivity and specificity of prognosis detection and evaluation of the gastric cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a biomarker for the diagnosis and prognosis evaluation of gastric cancer, a method for evaluating the prognosis of gastric cancer using the marker, and the application of a diagnostic kit, in particular to the tumor infiltrating immune cytokine IL23A as a biomarker for gastric cancer. The application of biomarkers and diagnostic kits for diagnosis, treatment and prognosis assessment. Background technique [0002] Gastric cancer (GC) is one of the most common malignant tumors in the world, ranking second in the number of deaths caused by cancer worldwide. According to data from the International Agency for Research on Cancer, there are about 1 million new cases of gastric cancer every year in the world, and more than 800,000 deaths among them. With the advancement of treatment and diagnostic technology, a variety of treatment and diagnosis methods are emerging, which have g...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574C12N15/11
CPCC12Q1/6886G01N33/57446C12Q2600/158
Inventor 康亚妮秦玉兰俞焙秦李华
Owner SHANGHAI JIAO TONG UNIV
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