Application of akkermansia muciniphila or prevotella copri in preparation of medicine for increasing gamma delta T cell accumulation in tumour microenvironment and improving anti-tumour immunity function

A technology of tumor microenvironment and Prevotella, applied in the field of biomedicine, to achieve the effect of inhibiting growth

Active Publication Date: 2020-07-28
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the use of intestinal bacteria including Akkermansia and / or Prevotella to increase the infiltration of γδT cells in the tumor microenvironment to enhance the anti-tumor function of the body

Method used

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  • Application of akkermansia muciniphila or prevotella copri in preparation of medicine for increasing gamma delta T cell accumulation in tumour microenvironment and improving anti-tumour immunity function
  • Application of akkermansia muciniphila or prevotella copri in preparation of medicine for increasing gamma delta T cell accumulation in tumour microenvironment and improving anti-tumour immunity function
  • Application of akkermansia muciniphila or prevotella copri in preparation of medicine for increasing gamma delta T cell accumulation in tumour microenvironment and improving anti-tumour immunity function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Ackerman's myxobacteria culture

[0043] training method

[0044] Step 1: Take a freeze-dried and preserved Akkermansia muciniphila strain (purchased from ATCC), add 200 μL TSB medium to dissolve it, take 200 μl blood plate to streak, and put it in an incubator under anaerobic conditions for 37 Cultivate for 48 hours at ℃;

[0045] Step 2: Pick a monoclonal colony and dissolve it in 10 ml of TSB medium, and culture it under anaerobic conditions at 37°C for 48 hours;

[0046] Step 3: Take 500 milliliters of TSB culture medium, inoculate strains according to 1% (v / v), and cultivate under anaerobic conditions at 37°C for 48 hours;

[0047] Step 4: Collect the bacterial liquid and centrifuge at 6000rpm for 10 minutes. After washing twice with normal saline, the bacteria sludge was reconstituted and counted viable bacteria.

Embodiment 2

[0048] Embodiment 2 Prevotella culture

[0049] training method

[0050] Step 1: Take a freeze-dried Prevotella copri strain (purchased from ATCC), add 200 μl of LPYG medium to dissolve it, take 200 μl of blood plate, and culture it in an incubator at 37°C for 48 hours under anaerobic conditions. Hour;

[0051] Step 2: Pick a monoclonal colony and dissolve it in 10 ml of TSB medium, and culture it under anaerobic conditions at 37°C for 48 hours;

[0052] Step 3: Take 500 milliliters of PYG culture medium, inoculate strains at 1% (v / v), and culture under anaerobic conditions at 37°C for 48 hours;

[0053] Step 4: Collect the bacterial liquid and centrifuge at 6000rpm for 10 minutes. After washing twice with normal saline, the bacteria sludge was reconstituted and counted viable bacteria.

Embodiment 3

[0054] Example 3 Akkermansia enhances the infiltration of γδT cells in the tumor microenvironment and treats tumor (liver cancer) experiment

[0055] figure 1 Schematic diagram of the experimental flow for testing Akkermansia or inactivated Akkermansia to increase γδ T cell infiltration and therapy in the tumor microenvironment.

[0056] 1. Training method

[0057] Akerman's myxobacteria culture method is the same as above-mentioned embodiment 1.

[0058] 2. Sample preparation

[0059] 1) Preparation of Ackermannian myxobacteria live cells

[0060] Step 1: Take a freeze-dried and preserved Akkermansia muciniphila strain (purchased from ATCC), add 200 μL TSB medium to dissolve it, take 200 μl blood plate to streak, and put it in an incubator under anaerobic conditions for 37 Cultivate for 48 hours at ℃;

[0061] Step 2: Pick a monoclonal colony and dissolve it in 10 ml of TSB medium, and culture it under anaerobic conditions at 37°C for 48 hours;

[0062] Step 3: Take 500...

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Abstract

The invention belongs to the field of biological medicines, and in particular relates to application of akkermansia muciniphila or prevotella copri and a composition containing akkermansia muciniphilaor prevotella copri in preparation of a medicine for resisting tumours and increasing infiltration of gamma delta T cells in a tumour microenvironment. The akkermansia muciniphila or prevotella copriand the composition containing akkermansia muciniphila or prevotella copri are used for preparing an anti-tumour medicine and increasing infiltration of the gamma delta T cells in the tumour microenvironment; therefore, tumour growth can be obviously inhibited; and furthermore, infiltration of the gamma delta T cells in the tumour microenvironment can be obviously increased. The invention furtherprovides application of akkermansia muciniphila or prevotella copri in preparation of pharmaceutical compositions, foods, health-care products and food additives for resisting tumours and increasinginfiltration of the gamma delta T cells in the tumour microenvironment.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the use of Akkermansia (or Akkermans for short) or Prevotella or a composition containing Akkermansia or Prevotella for anti-tumor and the increase of γδT cells (TCR-γδ positive T cells, TCR γδ positive T cells or TCR-γδ+T cells) in the application of drugs for infiltration in the tumor microenvironment. Background technique [0002] So far, malignant tumors are still the main killer threatening human health. For example, liver cancer is a kind of malignant tumor with extremely high degree of malignancy, low cure rate and poor prognosis. The incidence rate of liver cancer in the world is the fifth among men, and the death rate is the second; The death rate occupies the 9th place, and the death rate occupies the 6th place. China is a country with a high incidence of liver cancer, and the death toll accounts for more than 50% of the global death toll. Traditional treatment met...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61P35/00
CPCA61K35/741A61P35/00A61K2300/00
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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