pcr amplification instrument

Abstract

The application discloses a PCR amplification instrument, which includes a microprocessor and a power supply located in a sealed pressure-resistant water-tight compartment, and a flow-type PCR system located in a wet compartment sealed through an oil phase. The flow-type PCR system includes a micropipe, a micropump, a solenoid valve, and a temperature cycle device; both the micropump and the solenoid valve are connected to the micropipe; the micropump realizes the balance of the internal pressure from the external seawater pressure based on the principle of self-balancing pressure; the temperature cycle device The gap is filled with flexible pressure-resistant materials; the power supply is used to power the flow PCR system and the microprocessor; the microprocessor is used to drive the fluid to flow in the micropipe according to the preset flow speed and the preset flow direction by controlling the micropump and the solenoid valve. Control the automatic addition of PCR reagents and samples; it is also used to control the temperature rise and fall cycle of the temperature cycle device, so as to effectively realize the real-time monitoring of the microbial population in the deep sea in situ.

Description

technical field [0001] The present application relates to the technical field of equipment manufacturing based on polymerase chain reaction technology, in particular to a PCR amplification instrument. Background technique [0002] PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) is a molecular biology technique used to amplify and amplify a specific DNA fragment. Single-stranded DNA; when the DNA decomposed into a single strand drops to about 60°C, the single-stranded DNA will bind to the primer; under the action of DNA polymerase, the DNA bound to the primer will undergo semi-conservative replication according to the principle of complementary base pairing. Once the replication is complete, twice as much DNA is available. After multiple cycles of temperature at 95°C and 60°C, a large amount of DNA can be amplified. PCR technology based on the principle of PCR, as the most important molecular biology detection method today, can quickly identify bacteria, viruses,...

AI Technical Summary

Problems solved by technology

At present, all PCR instruments belong to the category of desktop instruments, which cannot meet the requirements of use in deep sea, field and other environments and the needs of long-term nucleic acid in situ detection

With the research needs of deep-sea microorganisms, and the special environment of the deep sea, the microorganisms and substances in it will change after leaving the in-situ environment, and more than 90% of the microorganisms in the deep sea are currently uncultivable due to reasons such as leaving the in-situ environment.

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