Rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water and application of rhodococcus ruber HDRR2Y

A technology of Rhodococcus rhodochrous and inorganic nitrogen, which is applied in the field of Rhodococcus rhodochrous HDRR2Y, can solve the problems of difficulty in separating and purifying nitrifying bacteria and denitrifying bacteria, staying on a small scale, failing to achieve the effect of large-scale industrial application of directional cultivation of single microorganisms, etc. Good environmental adaptability, reduced water body replacement, and remarkable removal effect

Active Publication Date: 2020-07-31
SHENZHEN BASE OF SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because nitrifying bacteria and denitrifying bacteria are relatively difficult to separate and purify, most researchers at home and abroad use activated sludge for enrichment culture, and seldom use pure bacteria to expand the culture method, and form pure bacteria that can be used in aquaculture industry on a large scale Preparation products are rarely reported (Yang Ning, 2003; Wang Juan, 2006)
Although microbial immobilization technology has attracted much attention and many beneficial explorations and attempts have been made, most of them are still in the stage of small-scale and small-scale application experiments, and have not yet achieved the effect of large-scale industrial application of directional cultivation of a single microorganism.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water and application of rhodococcus ruber HDRR2Y
  • Rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water and application of rhodococcus ruber HDRR2Y
  • Rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water and application of rhodococcus ruber HDRR2Y

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Screening and cultivation of Rhodococcus rhodococcus HDRR2Y for purifying seawater pond culture tail water inorganic nitrogen and phosphorus

[0030] 1. Material preparation

[0031] 1.1. Bacterial source

[0032] In the intensive prawn farming area of ​​Touwei Village, Ganggang Town, Huidong, Guangdong, the tail water samples from 60-90 days of cultivation were collected and separated and cultured on selective medium plates.

[0033] 1.2. Medium

[0034] (1) Selective liquid medium: CH 3 COONa: 1g, yeast extract: 1g, MgSO 4 ·7H 2 O: 0.4g, NaCl: 0.1g, CaCl 2 2H 2 O: 0.05g, NaHCO 3 : 0.3g, KH 2 PO 4 : 1g, 1mL of trace element solution, the above drugs were dissolved in distilled water, and fixed to 1000mL, pH7.0.

[0035] Trace element solution: EDTA: 2.5g, ZnSO 4 ·7H 2 O: 10.95g, MnSO 4 ·H 2 O: 1.54g, CuS0 4 ·5H 2 O: 0.39g, CoCl 2 ·6H 2 O: 0.2g, FeSO 4 ·7H 2O: 7g, glutamic acid: 0.02g, the above drugs were respectively dissolved in distille...

Embodiment 2

[0041] Example 2 Identification of the screened Rhodococcus rhodococcus HDRR2Y

[0042] The present invention carried out 16S rDNA molecular identification on the screened Rhodococcus rubrum HDRR2Y, and determined the species of the strain from the molecular level, combined with analysis of bacterial morphological characteristics and physiological and biochemical characteristics. 16S rDNA sequence analysis mainly follows the following steps:

[0043] 1. Extraction of bacterial genomic DNA:

[0044] (1) Use a sterile toothpick to pick a single colony and inoculate it in the expansion medium for cultivation;

[0045] (2) Take 1.5 mL of bacterial culture solution, centrifuge at 10,000 rpm (11,500×g) for 1 minute, and suck up the supernatant as much as possible;

[0046] (3) Add 200 μL of buffer GA to the cell pellet, shake until the cell is completely suspended, add 180 μL of lysozyme with a final concentration of 20 mg / mL, and treat at 37°C for more than 30 minutes;

[0047] ...

Embodiment 3

[0068] Example 3 Effect of Rhodococcus Erythrococcus HDRR2Y on Inorganic Nitrogen and Phosphorus Removal from Seawater Pond Culture Tail Water

[0069] 1. Growth of the strain

[0070] The bacterial strain Rhodococcus rubrum HDRR2Y that embodiment 1 obtains presses initial concentration 10 4 ~10 6 CFU / mL was inoculated into the tail water of sterilized intensive pond culture, and the strain could enter the plateau stage after about 2 days of growth, and the bacterial count reached 5.1×10 8 CFU / mL, remained stable at 6.3×10 on day 4 8 CFU / mL, the growth curve of Rhodococcus rhodococcus HDRR2Y is as follows figure 1 shown in .

[0071] 2. Inorganic nitrogen and phosphorus removal effect of strains in different salinity aquaculture tail waters

[0072] The tail water of the sterilized prawn intensive culture pond (water salinity 25) was used as the basic test water control, and Rhodococcus rhodococcus HDRR2Y was not added during the test. The bacterium-adding group adjusts ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses rhodococcus ruber HDRR2Y for removing inorganic nitrogen and phosphorus in seawater pond culture tail water. The rhodococcus ruber HDRR2Y has a preservation number of CCTCC M20191009, the preservation date is December 4, 2019, the preservation name is Rhodococcus ruber HDRR2Y, the preservation authority is China Center for Type Culture Collection, and the preservation address is Wuhan, China. The strain HDRR2Y has relatively strong removing capability on inorganic nitrogen and phosphorus in aquaculture water or tail water, is good in environmental adaptability, and hasno adverse effect on cultured fishes and shrimps. The invention further discloses an application of the rhodococcus ruber HDRR2Y in removing inorganic nitrogen and phosphorus in seawater pond culturetail water.

Description

technical field [0001] The invention belongs to the technical field of microbial purification of seawater pond culture water, and in particular relates to a Rhodococcus rhodococcus HDRR2Y for purifying inorganic nitrogen and phosphorus in seawater pond culture tail water and an application thereof. Background technique [0002] In 2018, the total output of aquaculture in my country exceeded 50 million tons, accounting for more than 78% of the total supply of aquatic products. If the aquaculture process is not properly managed, residual feed, aquaculture metabolites and aquatic biological residues will accumulate in the water environment, forming self-sourced aquaculture tail water or pollutants. In terms of the form of chemical substances contained in the tail water, there are mainly ammonia nitrogen, total inorganic nitrogen (TIN), phosphorus, organic matter and fouling organisms (Xu Sheng, 2011; Shu Tingfei et al., 2002). For example, harmful nitrogen such as ammonia nitr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F101/10C02F101/16
CPCC02F3/34C02F2103/20C02F2101/105C02F2101/16C12R2001/01C12N1/205
Inventor 曹煜成胡晓娟文国樑徐煜苏浩昌徐武杰杨铿许云娜虞为
Owner SHENZHEN BASE OF SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap