MOFs type molecularly imprinted polymer, preparation method thereof and pesticide residue fluorescence detection method

A technology of molecular imprinting and polymers, applied in fluorescence/phosphorescence, chemical instruments and methods, analytical materials, etc., can solve the problems of long cycle, single type of detection, high false positive and false negative rate of detection, etc.

Active Publication Date: 2020-08-04
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the preparation of biorecognition molecules is difficult, the cycle is long, and the cost of detection is relatively high; it is easily interfered by complex matrices, has poor stability and repeatability, and the detection rate of false positives and false negatives is high; Monitoring and early warning needs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MOFs type molecularly imprinted polymer, preparation method thereof and pesticide residue fluorescence detection method
  • MOFs type molecularly imprinted polymer, preparation method thereof and pesticide residue fluorescence detection method
  • MOFs type molecularly imprinted polymer, preparation method thereof and pesticide residue fluorescence detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] This embodiment provides a method for fluorescent detection of pesticide residues, which includes the following steps:

[0092] S1: FeCl 3 ·6H 2 O, FeCl 2 4H 2 O and high-purity water are mixed according to a certain volume ratio and placed in a 250mL three-necked bottle for ultrasonic dispersion for 0.5h; magnetically stirred for 0.5-1h at a temperature of 50-100°C, and a certain volume of NH with a mass fraction of 28% is added 3 ·H 2 O, continue to react for 1 h under the protection of nitrogen; after magnetic separation, use absolute ethanol and pure water to alternately wash several times, and vacuum dry the obtained magnetic nanoparticles Fe 3 o 4 NPs; among them, FeCl 3 ·6H 2 O, FeCl 2 4H 2 The consumption ratio of O, high-purity water and ammoniacal liquor is 0.5g:0.15g:200mL:5mL; The used dehydrated alcohol and high-purity water volume ratio of each washing are 10mL:15-30mL;

[0093] S2: The magnetic nanoparticles Fe 3 o 4 Add NPs and high-purity wa...

Embodiment 2

[0099] It differs from Example 1 in that:

[0100] In step S1, FeCl 3 ·6H 2 O, FeCl 2 4H2 The consumption ratio of O, high-purity water and ammoniacal liquor is 0.6g:0.35g:200mL:10mL; The used dehydrated alcohol and high-purity water volume ratio of each washing are 10mL:25mL.

[0101] In step S2, the magnetic nanoparticles Fe 3 o 4 NPs, high-purity water, FeCl 3 ·6H 2 O, FeCl 3 ·6H 2 O aqueous solution, H 2 The dosage ratio of BDC and DMF is 0.2g:200mL:0.45g:80mL:0.174g:50mL.

[0102] In step S3, the magnetic MOFs composite Fe 3 o 4 - The dosages of MIL-Fe, methanol, acetamiprid molar mass, clothianidin molar mass, APTES, TEOS and acetic acid solution are 0.4g:10mL:0.15mM:0.15mM:0.2mL:2mL, respectively.

Embodiment 3

[0104] It differs from Example 1 in that:

[0105] In step S1, FeCl 3 ·6H 2 O, FeCl 2 4H 2 The consumption ratio of O, high-purity water and ammoniacal liquor is 0.75g:0.45g:200mL:15mL; The used dehydrated alcohol and high-purity water volume ratio of each washing are 10mL:30mL.

[0106] In step S2, the magnetic nanoparticles Fe 3 o 4 NPs, high-purity water, FeCl 3 ·6H 2 O, FeCl 3 ·6H 2 O aqueous solution, H 2 The dosage ratio of BDC and DMF is 0.3g:200mL:0.9g:120mL:0.498g:100mL.

[0107] In step S3, the magnetic MOFs composite Fe 3 o 4 - The dosages of MIL-Fe, methanol, acetamiprid molar mass, clothianidin molar mass, APTES, TEOS and acetic acid solution are 0.5g:10mL:0.2mM:0.2mM:0.4mL:4mL, respectively.

[0108] In conjunction with above-mentioned embodiment and accompanying drawing, refer to figure 1 , MIL-Fe is a blocky three-dimensional highly cross-linked polymer with a non-smooth surface and irregular corners; when Fe 3 o 4 Fe prepared after doping modif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
specific surface areaaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses an MOFs type molecularly imprinted polymer, a preparation method thereof and a pesticide residue fluorescence detection method, and relates to the technical field of pesticidedetection. According to the method, MOFs are used as functional carriers; double-template imprinting polymerization is carried out on the surface of the metal organic framework to obtain a metal organic framework/molecularly imprinted polymer (MOFs type molecularly imprinted polymer Fe3O4-MIL-Fe-coated MIPs); the product has the advantages of adjustable pore diameter and high specific surface area; the characteristics of surface molecular imprinting rapid adsorption and high selective recognition are also realized; catalase catalysis and bionic recognition characteristics can be combined; based on the relative linear relationship between the concentration Log value of the neonicotinoid pesticide residue and the fluorescence intensity of the solution, a fluorescence rapid detection method for the neonicotinoid pesticide residue based on MOFs type molecular imprinting is constructed, so that fluorescence rapid detection of the pesticide residue is realized, and the detection efficiency is effectively improved.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a MOFs type molecularly imprinted polymer, a preparation method thereof, and a fluorescence detection method for pesticide residues. Background technique [0002] There are currently 8 types of neonicotinoid pesticides that have been used on the market, namely imidacloprid, acetamiprid, dinotefuran, nitenpyram, clothianidin, thiacloprid, thiamethoxam, and piperacrid. Neonicotinoid pesticides have been widely used in vegetables in recent years because of their broad insecticidal spectrum and low interaction with traditional pesticides. With the sharp increase in usage, the risk of people being exposed to neonicotinoid pesticides through contaminated vegetables has also increased accordingly. The quality and safety of vegetable products that may be caused by neonicotinoid pesticide residues has attracted more and more attention from the government and consumers. At pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00C08K3/22C08J9/26B01J20/26B01J20/30G01N21/64
CPCC08G83/008C08K3/22C08J9/26B01J20/268G01N21/643C08K2003/2275C08K2201/01
Inventor 刘广洋徐东辉高明坤李凌云黄晓冬许晓敏林桓张延国
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap