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Epimedium sagittatum petiole tissue culture and rapid propagation method

A technique for rapid propagation by tissue culture of Epimedium arrowefolium and tissue culture, applied to the field of rapid propagation by tissue culture of Epimedium arrowroot leaf petiole, can solve problems such as unreported, and achieve the effects of high reproduction rate, stable and excellent traits, and low cost.

Active Publication Date: 2020-08-11
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, tissue culture technology is rarely used in the production of Epimedium sagittatum seedlings, and there is no report on the method of tissue culture and rapid propagation of Epimedium sagittarius petioles as explants

Method used

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  • Epimedium sagittatum petiole tissue culture and rapid propagation method
  • Epimedium sagittatum petiole tissue culture and rapid propagation method
  • Epimedium sagittatum petiole tissue culture and rapid propagation method

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Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: a kind of epimedium sagittata petiole tissue culture rapid propagation method, its steps are as follows:

[0045] (1) Disinfection of petiole: collect fresh, healthy and disease-free petiole from the Epimedium sagittatum plant in the transition nursery of Wuhan Botanical Garden, Chinese Academy of Sciences, cut into small sections of about 2 or 2.2 or 2.5 cm, and place the explant material in 1: Soak in 800% chlorothalonil wettable powder aqueous solution for 15 minutes, then remove the petiole and move it to a 500ml large beaker, cover it with gauze, place it under tap water, rinse it for 1 hour, drain it, and finally transfer it to a clean bench with 75% alcohol The solution was sterilized for 25 or 30 or 35 seconds, during which time it was gently stirred with sterilized tweezers, taken out and rinsed with sterile water for 3 or 4 times to remove residual alcohol; then sterilized with 0.1% mercury liter for 8 minutes, rinsed with sterile water for 3 or ...

Embodiment 2

[0051] Embodiment 2: a kind of epimedium sagittata petiole tissue culture rapid propagation method, its steps are as follows:

[0052] (1) Disinfection of petiole: collect fresh, healthy and disease-free petiole from the Epimedium sagittatum plant in the transition nursery of Wuhan Botanical Garden, Chinese Academy of Sciences, cut into small sections of about 2 or 2.3 or 2.5 cm, and place the explant material in 1: Soak in 800% chlorothalonil wettable powder aqueous solution for 15 minutes, then remove the petiole and move it to a 500ml large beaker, cover it with gauze, place it under tap water, rinse it for 1 hour, drain it, and finally transfer it to a clean bench with 75% alcohol The solution was sterilized for 25 or 30 or 35 seconds, during which time it was gently stirred with sterilized tweezers, taken out and rinsed with sterile water for 3 or 4 times to remove residual alcohol; then sterilized with 0.1% mercury liter for 8 minutes, rinsed with sterile water for 3 or ...

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Abstract

The invention discloses an epimedium sagittatum petiole tissue culture and rapid propagation method. A complete plant is formed by a series of the steps of inducing calluses by petioles, performing differentiating to form adventitious buds or cluster buds, and performing inducing to generate fibrous roots, and then robust seedlings suitable for natural conditions are formed by seedling hardening and seedling raising treatment. The method has the advantages of being easy to operate, long in material taking time, high in propagation rate, short in propagation cycle, low in cost and high in survival rate after seedling hardening, is suitable for large-scale industrial production, and has a promoting effect on sustainable utilization of epimedium resources.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically relates to a method for tissue culture and rapid propagation of epimedium sagittarius petiole. Background technique [0002] Epimedium is a traditional Chinese medicine commonly used in my country. It has the functions of nourishing kidney yang, strengthening muscles and bones, improving immunity, anti-cancer, and protecting nerves. The medicines based on it are involved in various dosage forms, and there are dozens of them. "Chinese Pharmacopoeia" included 5 species of Epimedium genus, among which Epimedium sagitta is the most widely distributed species, widely distributed in East China, Central China and Southwest my country provinces, with a large amount of use. With the plundering and excavation of the wild resources of Epimedium sagittatum, the wild resources are severely damaged, and artificial planting is imminent. However, the traditional propagation me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张燕君黄天悦于淑霞梁琼于东悦王兰香
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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