Epimedium sagittatum petiole tissue culture and rapid propagation method
A technique for rapid propagation by tissue culture of Epimedium arrowefolium and tissue culture, applied to the field of rapid propagation by tissue culture of Epimedium arrowroot leaf petiole, can solve problems such as unreported, and achieve the effects of high reproduction rate, stable and excellent traits, and low cost.
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Embodiment 1
[0044] Embodiment 1: a kind of epimedium sagittata petiole tissue culture rapid propagation method, its steps are as follows:
[0045] (1) Disinfection of petiole: collect fresh, healthy and disease-free petiole from the Epimedium sagittatum plant in the transition nursery of Wuhan Botanical Garden, Chinese Academy of Sciences, cut into small sections of about 2 or 2.2 or 2.5 cm, and place the explant material in 1: Soak in 800% chlorothalonil wettable powder aqueous solution for 15 minutes, then remove the petiole and move it to a 500ml large beaker, cover it with gauze, place it under tap water, rinse it for 1 hour, drain it, and finally transfer it to a clean bench with 75% alcohol The solution was sterilized for 25 or 30 or 35 seconds, during which time it was gently stirred with sterilized tweezers, taken out and rinsed with sterile water for 3 or 4 times to remove residual alcohol; then sterilized with 0.1% mercury liter for 8 minutes, rinsed with sterile water for 3 or ...
Embodiment 2
[0051] Embodiment 2: a kind of epimedium sagittata petiole tissue culture rapid propagation method, its steps are as follows:
[0052] (1) Disinfection of petiole: collect fresh, healthy and disease-free petiole from the Epimedium sagittatum plant in the transition nursery of Wuhan Botanical Garden, Chinese Academy of Sciences, cut into small sections of about 2 or 2.3 or 2.5 cm, and place the explant material in 1: Soak in 800% chlorothalonil wettable powder aqueous solution for 15 minutes, then remove the petiole and move it to a 500ml large beaker, cover it with gauze, place it under tap water, rinse it for 1 hour, drain it, and finally transfer it to a clean bench with 75% alcohol The solution was sterilized for 25 or 30 or 35 seconds, during which time it was gently stirred with sterilized tweezers, taken out and rinsed with sterile water for 3 or 4 times to remove residual alcohol; then sterilized with 0.1% mercury liter for 8 minutes, rinsed with sterile water for 3 or ...
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