Preparation method of mesenchymal stem cells derived from early mesoderm

A technology of stromal stem cells and germ layer, applied in the field of mesenchymal stem cell preparation, can solve the problems of easily affecting the health of recipients, reducing the cell extraction rate, cell gene defects, etc., to promote adherent growth, reduce culture costs, and simplify the culture process. Effect

Inactive Publication Date: 2020-08-11
河南省遗传资源细胞库有限公司 +1
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Problems solved by technology

[0004] The cells extracted by the previous stem cell extraction method may have genetic defects, which may easily affect the health of the recipient. At the same time, the temperature change during the cell extraction process may easily inactivate the cells, and there is a problem of reducing the extraction rate of the cells.

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  • Preparation method of mesenchymal stem cells derived from early mesoderm

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] see figure 1 , a method for preparing mesenchymal stem cells derived from early developmental mesoderm, comprising the following steps:

[0026] Step A: Cleaning, extract the umbilical cord, wash the umbilical cord with distilled water, and wipe it with a sterile sponge;

[0027] Step B: enzymatic hydrolysis, take out the umbilical cord and crush it, and use 1% collagenase for enzymatic hydrolysis for 7 hours;

[0028] Step C: Filtration, bring 5 time...

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Abstract

The invention discloses a preparation method of mesenchymal stem cells derived from early mesoderm. The method comprises the steps of cleaning, enzymolysis, filtering, primary culture, subculture, induced differentiation and induced differentiation. The preparation method has the beneficial effects that the separation efficiency of the stem cells is improved, so that temperature change during heating and refrigeration of the stem cells becomes more smooth, cell inactivation caused by the temperature change in a cell extraction process is avoided, the problem of the extraction rate of the cellsis reduced, the separation speed of the mesenchymal stem cells can be improved, purity of the mesenchymal stem cells in a separation process is ensured by polylysine, and growth of the cells adheringto walls after separation is promoted.

Description

technical field [0001] The invention relates to a method for preparing cells, in particular to a method for preparing mesenchymal stem cells derived from mesoderm in early development, and belongs to the technical field of cell preparation. Background technique [0002] Mesenchymal stem cells are a kind of stem cells with multi-directional differentiation potential derived from mesoderm and ectoderm in early development, which can be induced to differentiate into skeletal muscle, smooth muscle, bone, cartilage, fat, cardiomyocytes, nerve cells and hematopoietic stem cells. It is a potential target cell for gene therapy and stem cell research. Human umbilical cord mesenchymal stem cells have the advantages of wide source, easy access, strong proliferation ability, and no ethical issues. It can be used as a new type of seed cell. [0003] Studies have confirmed that human umbilical cord mesenchymal stem cells and their induced islet-like cells do not express cell surface mark...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00C12N2509/10
Inventor 韦丹朱学义张云国唐成王迎耀薛晓锋
Owner 河南省遗传资源细胞库有限公司
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