General circulating preparation, separation and purification device for gamma cyclodextrin and sugammadex

Abstract

The invention discloses a general circulating preparation, separation and purification device for gamma cyclodextrin and sugammadex. A second mobile phase output end, the input end of a second pump, afraction collection port and the output port of a detector are connected to a first switching valve; the first end of a second column, the output end of a second pump and a waste liquid collection port are connected to a second switching valve; the second end of the second column and a first column are connected to a third switching valve; a first mobile phase output end, a first pump input end and the input port of the detector are connected to a fourth switching valve, a first pump output end is connected to the first end of the first column, the second end of the second column and a secondpump output end, the waste liquid collection port is connected to the second switching valve, one valve port of the second switching valve and one valve port of the third switching valve are directlycommunicated, and one valve port of the third switching valve and one valve port of the fourth switching valve are directly communicated. According to the device, two samples of gamma-cyclodextrin and sugammadex structures can be well separated and purified at the same time, enrichment in a short time can be achieved, elution conditions and switching valve purification and even other types of compounds can be changed, and the application prospect is good.

Description

technical field [0001] The invention relates to the fields of instrument equipment, instrument analysis, and drug research, in particular to a general-purpose circulation preparation and separation and purification device for gamma cyclodextrin and sugammadex. Background technique [0002] Gamma cyclodextrin (γ-Cyclodextrin) is a cyclic oligosaccharide composed of 8 D-glucopyranose units. The main molecule of gamma cyclodextrin can form a conical cylinder with a wide top and a narrow bottom with open ends. This cavity can contain a wide range of guest molecules, thereby improving the solubility of the guest molecules and increasing the stability of the guest molecules. Therefore, gamma cyclodextrin is widely used in the fields of medicine, cosmetics, food and chemical industry have been widely applied. [0003] Sugammadex is a modified derivative of gamma cyclodextrin. Its molecular structure retains gamma cyclodextrin and consists of a lipophilic core and a hydrophilic out...

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Problems solved by technology

[0005] At present, the methods of purifying the above two samples reported in the open literature are mostly activated carbon adsorption, recrystallization purification and preparative chromatographic purification. High polarity, almost no retention during preparative chromatography, and secondary purification cannot be achieved after the first purification, so the purification cost is high, the amount of solvent is large, and the usual preparative chromatography cannot be scaled up in batches

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