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Bordetella pertussis nucleic acid detection kit

A detection kit and pertussis technology, applied in the field of kits, can solve the problems of low sensitivity, low sensitivity, and long sampling cycle, etc., and achieve the effect of meeting the experimental requirements, good repeatability, and good sensitivity

Pending Publication Date: 2020-08-21
湖南宏雅基因技术有限公司
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AI Technical Summary

Problems solved by technology

[0003] The methods for detecting Bacillus pertussis in existing traditional schemes mainly include the following: 1. Pertussis culture is the gold standard for pertussis detection, and its specificity is strong, but its culture period is long (generally about 7 days), and its sensitivity is low and it is not suitable for culture techniques. Suitable for rapid detection in laboratories
2. The immunological detection method of whooping cough usually takes two serum samples from the suspected acute stage and the convalescent stage for detection. The sampling cycle is relatively long, and the sensitivity and specificity are not high, so it is not suitable for the rapid diagnosis of whooping cough
3. The rapid diagnosis of pertussis is PCR diagnosis, but the sensitivity of the primers currently used is not high, and it is not suitable for detecting Bacillus pertussis nucleic acid in nasopharyngeal swab samples

Method used

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  • Bordetella pertussis nucleic acid detection kit
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  • Bordetella pertussis nucleic acid detection kit

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Embodiment Construction

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below, and the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used can be obtained from commercial sources unless otherwise specified.

[0020] The B. pertussis nucleic acid detection kit includes multiple sequences: the upstream primer shown in SEQ ID NO: 01, the downstream primer shown in SEQ ID NO: 02, the probe shown in SEQ ID NO: 03, the sequence shown in SEQ ID NO: The internal standard shown in 07, the internal standard upstream primer shown in SEQ ID NO: 04, the internal standard downstream primer shown in SEQ ID NO: 05, the internal standard probe shown in SEQ ID NO: 06; and real-time fluorescent quantitative PCR Reagents required for detection. Reagents sho...

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Abstract

The invention provides a bordetella pertussis nucleic acid detection kit. The bordetella pertussis nucleic acid detection kit comprises a probe for detecting target polynucleotide, and an upstream primer and a downstream primer for amplifying the target polynucleotide. The bordetella pertussis nucleic acid detection kit provided by the invention solves the technical problem of low sensitivity of primers adopted for rapid diagnosis of PCR of bordetella pertussis in the prior art.

Description

technical field [0001] The invention relates to the field of kits, in particular to a detection kit for bacillus pertussis nucleic acid. Background technique [0002] Bacillus pertussis is an obligate aerobic Gram-negative small bacillus belonging to the genus Bordetella, with no spores and no flagella. The newly isolated strain is smooth, capsulated and virulent. The only host of pertussis bacillus is human beings. Pertussis bacillus mainly causes pertussis, which is an acute respiratory disease, highly contagious, and the crowd is generally susceptible, especially in infants and young children. It is one of the main infectious diseases that seriously threaten human health. one. [0003] The methods for detecting Bacillus pertussis in existing traditional schemes mainly include the following: 1. Pertussis culture is the gold standard for pertussis detection, and its specificity is strong, but its culture period is long (generally about 7 days), and its sensitivity is low a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/04C12R1/01
CPCC12Q1/689C12Q1/6851C12Q2600/166C12Q2521/531C12Q2531/113C12Q2545/101
Inventor 曹宇
Owner 湖南宏雅基因技术有限公司