Automatic measurement method for tangential adhesion of cells

Abstract

The invention discloses an automatic measurement method for tangential adhesion of cells. The method comprises the following steps of: arranging a cell measurement area and a needle washing area in asame culture dish, and moving a microtubule to the cell measurement area after the microtubule is rinsed in the needle washing area; automatically moving the microtubule to a position above the cell,and moving the gas-liquid interface to the front end of the microtubule; carrying out automatic contact detection in the descending process of the microtubule, and automatically stopping descending ofthe microtubule when the microtubule is in contact with the surface of the cell; reducing the equilibrium pressure in the microtubule, recording the equilibrium pressure values when the cells and themicrotubule are just sealed and when the cells are just sucked into the microtubule, and calculating the tangential adhesion of the cells. According to the invention, the cell measuring area and theneedle washing area are arranged in the same culture dish, so that the step of removing the microtubule for rinsing is omitted; whether the microtubule is in contact with cells or not is judged by detecting the movement condition of the microtubule in the descending process, and the influence of human factors is eliminated; adsorption force is measured through the equilibrium pressure, tangentialadhesion of cells is calculated, and automatic measurement of the tangential adhesion of cells is achieved.

Description

technical field [0001] The invention belongs to the technical field of cell-level measurement, in particular to an automatic measurement method for cell tangential adhesion. Background technique [0002] Cell adhesion is closely related to physiological processes such as cell growth and differentiation. Measuring cell adhesion is of great significance in the medical fields such as studying the mechanical properties of cells, exploring the process of tumor formation and even cell carcinogenesis. At present, there are various methods for measuring cell adhesion, such as microtube holding method, optical tweezers method, magnetic tweezers method, environmental scanning electron microscope, atomic force microscope and other techniques. Among them, the microtube suction method is widely used because of its low equipment requirements, little damage to cells, and high integration with other instruments. [0003] The traditional microtube suction method is used to measure the tange...

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Problems solved by technology

[0003] The traditional microtube suction method measures the tangential adhesion of cells. In order to reduce the friction between the microtube and the cell, the microtube needs to be removed and rinsed in trypsin every time a cell is measured, which greatly reduces the efficiency of the measurement.

At the same time, during the measurement process, the relative height of the microtube tip and the cell at the holding position is judged entirely by the operator's experience, lacking an objective standard, so there are high requirements for the operator, and due to the influence of subjective factors There is a large uncertainty in the measurement results

In addition, in the traditional measurement process, the gas in the pneumatic injector and the liquid in the petri dish will form a gas-liquid interface in the microtube due to capillary force, and the liquid at the gas-liquid interface will be subject to capillary force , has a great influence on the measurement results

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