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Liquid chromatography method for detecting corresponding real object of paeonia lactiflora and liquorice decoction, standard fingerprint spectrum and application

A technology of Shaoyao Gancao decoction and liquid chromatography, applied in the field of analysis of traditional Chinese medicine

Active Publication Date: 2020-09-04
XIAMEN TRADITIONAL CHINESE MEDICINE
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no liquid chromatographic method for detecting the corresponding object of Shaoyao Gancao Decoction, the method of establishing the standard fingerprint of Shaoyao Gancao Decoction and the research on using it for quality control

Method used

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  • Liquid chromatography method for detecting corresponding real object of paeonia lactiflora and liquorice decoction, standard fingerprint spectrum and application
  • Liquid chromatography method for detecting corresponding real object of paeonia lactiflora and liquorice decoction, standard fingerprint spectrum and application
  • Liquid chromatography method for detecting corresponding real object of paeonia lactiflora and liquorice decoction, standard fingerprint spectrum and application

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Embodiment Construction

[0101] The present invention will be described in detail below in conjunction with technical solutions and drawings, but the present invention is not limited thereto.

[0102] 1 Shaoyao Gancao Decoction Corresponding Physical Preparation

[0103] Prescription composition: white peony 55.20g, roasted licorice 55.20g

[0104] Take the above prescription amount of decoction pieces in a 2L decoction pot, add 600ml of water, decoct for 60 minutes, filter while hot (200 mesh) to obtain the medicinal liquid, and dry it to obtain the dry powder of Shaoyao Gancao Decoction, which is the corresponding physical product of Shaoyao Gancao Decoction, brown yellow To tan powder, sealed in a ziplock bag, and stored in a dry cabinet.

[0105] Paeoniflorin, liquiritin, ammonium glycyrrhizinate, gallic acid, and catechin were all purchased from China National Institutes for Food and Drug Control; Formylpaeoniflorin and oxidized paeoniflorin were purchased from Shanghai Hongyong Biotechnology C...

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Abstract

The invention relates to a liquid chromatography method for detecting a real object corresponding to paeonia lactiflora and liquorice decoction, a standard fingerprint spectrum and application. According to the method, a high performance liquid chromatograph equipped with an ultraviolet detector is adopted for detection. Chromatographic conditions are as follows: chromatographic conditions are shown in the specification; a mobile phase: acetonitrile (A)-0.05% phosphoric acid aqueous solution (B); the gradient elution conditions are as follows: 14-17.5% of A in 0-1 min, 17.5-19% of A in 1-5 min, 19-20% of A in 5-6 min, 20% of A in 6-7 min, 20-20.5% of A in 7-8 min, 20.5-23% of A in 8-12 min, 23-36% of A in 12-30 min, 36% of A in 30-32 min, 36-43% of A in 32-45 min; a chromatographic column:Venusil XBP C18 is 4.6*150 mm, 3 [mu]m; wherein the detection wavelength is 230 to 240 nm; the flow rate is 1.0 ml / min; and the column temperature of 20-30 DEG C. The obtained standard fingerprint spectrum of the real object corresponding to the paeonia lactiflora and liquorice soup can be used for controlling the quality of the real object corresponding to the paeonia lactiflora and liquorice soup.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a liquid chromatography method for detecting corresponding objects of Shaoyao Gancao Decoction, a method for establishing a standard fingerprint including the method, a standard fingerprint of the corresponding object of Shaoyao Gancao Decoction and an application thereof. Background technique [0002] The corresponding physical product of Shaoyao Gancao Decoction is prepared by decocting Chinese herbal pieces with water and then freeze-drying according to the prescription. [0003] "Chinese Pharmacopoeia" is the code of Chinese drug quality standards, the legal basis for testing and judging whether the drug is qualified or not. The current version is the 2015 edition, and the 2 traditional Chinese medicine decoction pieces in Shaoyao Gancao Decoction are all recorded. The control of the main chemical components of the prescription decoction pieces in the Pharma...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8631G01N30/8686G01N2030/324
Inventor 关斌肖惠琳阳丽华许舒瑜陈晓琳南淑华郑珊珊许玉珍肖顺彪赖志成
Owner XIAMEN TRADITIONAL CHINESE MEDICINE
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