Method for efficiently and accurately detecting melatonin content in Paeonia lactiflora Pall

A technology of melatonin and peony, which is applied in the field of efficient and accurate detection of melatonin content in peony, can solve the problem of not finding it, and achieve the effect of simple operation, strong specificity, accurate and reliable results

Active Publication Date: 2016-12-07
YANGZHOU UNIV
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Problems solved by technology

Compared with HPLC-MS, high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has higher specifi

Method used

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  • Method for efficiently and accurately detecting melatonin content in Paeonia lactiflora Pall
  • Method for efficiently and accurately detecting melatonin content in Paeonia lactiflora Pall
  • Method for efficiently and accurately detecting melatonin content in Paeonia lactiflora Pall

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Embodiment 1

[0042] 1. Instruments and reagents: Agilent6460 liquid mass spectrometer (Agilent, USA), Syncronis AQ-C18 chromatographic column (Thermo, USA), vacuum centrifugal concentrator (Eppendorf, Germany), KQ-200VDB ultrasonic cleaner (Kunshan, Jiangsu, ultrasonic Instrument Co., Ltd.), HC-2518 centrifuge (HKUST Innovation Co., Ltd.); analytical balance (Beijing Sartorius Scientific Instrument Co., Ltd.); microporous membrane (0.22 μm) (Shanghai AMP Experimental Technology Co., Ltd.).

[0043] Melatonin standard (purity ≥ 98%, Sigma Company, USA), methanol (HPLC grade, Tedia Reagent Company, USA), acetonitrile (HPLC grade, Tedia Reagent Company, USA), and deionized water were prepared by Millipore ultrapure water system.

[0044] 2. Preparation of the sample solution to be tested: the peony cultivar 'Hongfeng' planted in the peony germplasm resource nursery (32°30'N, 119°25'E) of the School of Horticulture and Plant Protection of Yangzhou University in the field was used as a test mate...

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Abstract

The invention relates to a method for efficiently and accurately detecting melatonin content in Paeonia lactiflora Pall. The method comprises the following steps: preparing a reference solution, preparing a sample solution to be detected and carrying out high performance liquid chromatography-tandem mass spectrometry. The chromatographic peak of obtained melatonin detected in the Paeonia lactiflora Pall and the chromatographic peaks of other components can reach baseline separation, and are symmetrical in gauss peaks and good in peak patterns and can meet qualitative and quantitative requirements; the detection method of the melatonin content in the Paeonia lactiflora Pall provided by the invention is easy and simple to operate, high in sensitivity, strong in specificity, good in stability and accurate and reliable in results.

Description

technical field [0001] The invention relates to a method for efficiently and accurately detecting the content of melatonin in peonies. Background technique [0002] Peony (Paeonia lactiflora Pall.) is a perennial herbaceous plant of Paeoniaceae, which has high ornamental and medicinal value. The medicinal use of peony is mainly to extract its root system, and its aerial parts have no other use except for ornamental purposes, especially the petals. After the flowering period every year, the petals fall all over the ground, which has virtually caused a huge amount of resources. waste. Melatonin is an indole neuroendocrine hormone that has attracted much attention in recent years. It has many important physiological functions for the human body, such as improving sleep quality, enhancing immunity, slowing down aging, anti-tumor, Antioxidant and regulate circadian rhythm etc. Previous studies have shown that after eating fruits and vegetables rich in melatonin, the level of m...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陶俊赵大球刘定李志远陆春良
Owner YANGZHOU UNIV
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