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In-vitro culture method of asiatic toddalia root

A technique for in vitro culture of dragon palm blood, applied in the field of plant tissue culture, can solve problems such as hindering the large-scale use of dragon palm blood and research improvement.

Inactive Publication Date: 2020-09-08
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is no relevant record about the tissue culture of Feilongzhang blood, which hinders the large-scale use and research improvement of Feilongzhang blood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) explant collection: collect with scissors the sprouting tiller at the rhizome junction of the year's life Pterodactyl palmatum rhizome that is good in physiological state and free from damage by diseases and insect pests as explants;

[0033] (2) First-generation induction culture: the explants collected in step (1) were rinsed under tap water for 3 hours, placed in a clean bench and sterilized twice in 70% ethanol solution for 30 seconds each time, and then added 1-2 drops of Tween-20 0.1% HgCI 2 Stir and sterilize for 10 minutes, and finally rinse with sterile water for 7 times, blot dry with sterile filter paper, remove part of the leaflets, cut into 1.0-1.5cm long stems and inoculate them into the primary induction medium, place at 20°C and fully Adventitious buds can be induced to form after dark culture for 25 days, and the induction rate reaches 93%.

[0034] The induction medium is: Miller medium+3.0mg / L Vc+0.5mg / L PVP+0.1mg / L ZT+0.1mg / L IBA+0.5mg / L activat...

Embodiment 2

[0042] (1) Explant collection: collect with scissors the sprouting tillers at the joints of the rhizomes and rhizomes of the year-old Pterodactyl palmatum with good physiological state and no damage by diseases and insect pests as explants;

[0043] (2) First-generation induction culture: the explants collected in step (1) were rinsed under tap water for 3 hours, placed in a clean bench and sterilized twice in 70% ethanol solution, each time for 30 seconds, and then used With 0.1% HgCI adding 1-2 drops of Tween-20 2 Stir and sterilize for 10 minutes, finally rinse with sterile water for 5 times, then blot dry with sterile filter paper, remove part of the leaflets, cut into 1.0-1.5cm long stems and inoculate them into the primary induction medium, place at 20°C and fully Adventitious buds can be induced to form after dark culture for 30 days, and the induction rate reaches 91%.

[0044] The induction medium is: Miller medium+2.0mg / L Vc+0.3mg / L PVP+0.08mg / L ZT+0.05mg / L IBA+0.3m...

Embodiment 3

[0052] (1) explant collection: collect with scissors the sprouting tiller at the rhizome junction of the year's life Pterodactyl palmatum rhizome that is good in physiological state and free from damage by diseases and insect pests as explants;

[0053] (2) First-generation induction culture: the explants collected in step (1) were rinsed under tap water for 3 hours, placed in a clean bench and sterilized twice in 70% ethanol solution for 30 seconds each time, and then added 1-2 drops of Tween-20 0.1% HgCI 2 Stir and sterilize for 10 minutes, and finally rinse with sterile water for 6 times, blot dry with sterile filter paper, remove part of the leaflets, cut into 1.0-1.5cm long stems and inoculate them into the primary induction medium, place at 20°C and fully Adventitious buds can be induced to form after dark culture for 35 days, and the induction rate reaches 85%.

[0054] The induction medium is: Miller medium+1.0mg / L Vc+0.1mg / L PVP+0.05mg / L ZT+0.01mg / L IBA+0.1mg / L activ...

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PUM

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Abstract

The invention discloses an in-vitro culture method of asiatic toddalia root, which belongs to the field of plant tissue culture and comprises the following steps: collecting sprout tillers of asiatictoddalia root as explants; culturing the explants after disinfection to obtain adventitious buds; primary induction culture of adventitious buds; carrying out subculture multiplication culture; rooting culture; transplantation. According to the method, a plant tissue culture technology is adopted to establish a asiatic toddalia root in-vitro culture method, sprout tillers at the rhizome joints ofasiatic toddalia roots and stems serve as explants, and the steps of primary induction culture, subculture multiplication culture, rooting culture, transplantation and the like are performed, so thatthe induction rate reaches 85% or above, and the multiplication coefficient reaches 4.5; and the transplanting survival rate reaches 90% or above. The method can be directly used for industrial production of the asiatic toddalia root seedlings, and provides a basis for subsequent genetic system establishment, genetic resource renewal of transgenosis and the like, and genetic improvement work.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for in vitro culture of dragon's palm blood. Background technique [0002] Toddalia asiatica Lam., commonly used by the Yao people, is a plant of the Rutaceae Toddalia asiatica Lam. Distributed in Shaanxi, Gansu, Zhejiang, Jiangxi, Fujian, Taiwan, Hubei, Hunan, Guangxi, Guangdong, Sichuan, Guizhou, Yunnan and other provinces. The effect of swelling and detoxification, the root bark is mainly used for bruises, rheumatoid arthritis, intercostal neuralgia, stomach pain, irregular menstruation, dysmenorrhea, and amenorrhea; external use for fractures, traumatic bleeding, external use for leaves to treat carbuncles, furuncles, swollen poison, snake bites . Since the 1990s, there have been many reports on the identification, chemical composition, and pharmacological effects of Feilongzhangxue. [0003] However, there is no relevant record about the tissue cultu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫昭展苏建睦
Owner YULIN NORMAL UNIVERSITY