Biomimetic nanomaterials and preparation methods thereof for sonodynamic/gas synergistic anti-tumor therapy
A biomimetic nano-acoustodynamic technology, applied in the field of biomimetic nano-materials, can solve the problems of low tumor enrichment efficiency, poor biocompatibility of nanocarriers, and reduced treatment efficiency, so as to improve enrichment efficiency and biocompatibility , Reduce the effect of phagocytosis and clearance
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Embodiment 1
[0031] Measure 6 mL of DMF dispersion containing 0.2 mg / mL black phosphorus nanosheet BPNs, in an ice-bath environment, use a cell sonicator at 70% output power for 12 h, and centrifuge at 2000 r / min for 5 min to remove The precipitate (that is, the BPNs that has not been stripped by ultrasound) was centrifuged in an ultracentrifuge at 40,000 r / min for 20 min, and the precipitate was taken to obtain 1.2 mg of black phosphorus quantum dots (BPQDs), which were resuspended in 1 mL of deionized water.
Embodiment 2
[0033] Weigh 2 g CTAC and 20 mg TEA respectively, add both to the BPQDs suspension prepared in Example 1, stir vigorously for 1 hour, then slowly drop 1.5 mL TEOS into the above mixed solution, and vigorously Stir for 1 hour, and after the solution drops to room temperature, centrifuge for 10 min at 12,000 r / min, take the precipitate and wash it with ethanol three times, then disperse it in 50 mL of methanol containing 1 wt% sodium chloride, stir for 3 hours, and centrifuge. The precipitation was repeatedly washed and stirred three times to remove the template CTAC and obtain black phosphorus quantum dot hybrid mesoporous silicon nanoparticles (BMSN).
[0034] Add the prepared BMSN and APTES to ethanol at a mass ratio of 5:1, stir vigorously at 80°C for 24 hours, centrifuge, wash the precipitate with ethanol three times, and resuspend with deionized water to obtain BMSN with a concentration of 3 mg / mL -NH 2 solution. 2 mL of BMSN-NH 2 The solution was mixed with 5 μL, 20 mg...
Embodiment 3
[0037] To lyse the J774A.1 cells, that is, to culture the J774A.1 cells in a culture dish first, and scrape them off with a cell scraper after the cells are congested; transfer the cells to a centrifuge tube after adding a small amount of PBS, and centrifuge at 300 g for 5 min to get the cell pellet, then add PBS to wash twice; then follow the operation steps of the membrane protein extraction kit (purchased from Beyontian Biotechnology Co., Ltd.) to extract the cell membrane, and store the extracted J774A.1 cell membrane at -80 ℃ spare. After BCA protein quantification, CAu-BMSN was mixed with macrophage cell membrane at a mass ratio of 5:1 and sonicated for 30 min to prepare N@CAu-BMSN.
[0038] The resulting N@CAu-BMSN was characterized by transmission electron microscopy, and detected by Coomassie brilliant blue and Western blot. The results are as follows figure 2 shown. Depend on figure 2 It can be seen from the middle transmission electron microscope image that the...
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