Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Radionuclide targeted breast cancer sentinel lymph node metastasis tracer and preparation method thereof

A radionuclide and sentinel lymph node technology, applied in the direction of radioactive preparations, radioactive carriers, and pharmaceutical formulations in the body, can solve problems that affect surgeons' surgical decisions, increase patients' medical economic expenditures, and increase the workload of pathologists, etc., to achieve reduction Effects of local trauma, shortened operation time, and reduced medical expenses

Pending Publication Date: 2020-10-13
王永胜
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors of the present invention found that the sentinel lymph node tracers (methylene blue, radionuclide and fluorescence) only have the effect of locating the sentinel lymph node, but do not have the effect of indicating whether there is a metastasis. Medical examination will greatly increase the workload of pathologists, and will also greatly increase the medical and economic expenditure of patients; in addition, intraoperative rapid frozen pathological examination and postoperative routine pathological diagnosis of sentinel lymph nodes will take a long time, which will be extremely serious. Significantly prolongs operating time and affects surgeons' surgical decisions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Radionuclide targeted breast cancer sentinel lymph node metastasis tracer and preparation method thereof
  • Radionuclide targeted breast cancer sentinel lymph node metastasis tracer and preparation method thereof
  • Radionuclide targeted breast cancer sentinel lymph node metastasis tracer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Prepare a mixed solution of CK19 monoclonal antibody and 2-mercaptoethanol.

[0032] (2) Prepare lyophilized products of CK19 monoclonal antibody, 2-mercaptoethanol, and small molecule dextran;

[0033] (3) Radioactive 99m Tc labeling on CK19 monoclonal antibody to prepare a new type of tracer 99m Tc-CK19 monoclonal antibody;

[0034] (4) Detection 99m Radiochemical purity of Tc-CK19 monoclonal antibody, and detection of CK19 monoclonal antibody protein molecular integrity and immune activity;

[0035] (5) will 99m The Tc-CK19 monoclonal antibody was injected into the subcutaneous tissue of the affected side of breast cancer patients, and the radioactive concentration of the lymph nodes in the ipsilateral axillary region was continuously detected in vivo by a radioactive gamma ray detector, and the presence or absence of lymph nodes in the lymph nodes was judged according to the radioactive concentration of the axillary lymph nodes. Breast cancer metastases. I...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
radioactivityaaaaaaaaaa
Login to View More

Abstract

The invention provides a radionuclide targeted breast cancer sentinel lymph node metastasis tracer and a preparation method thereof. The tracer comprises a cytokeratin 19 (CK19) monoclonal antibody and pertechnetate, and the pertechnetate is subjected to a reaction through 2-mercaptoethanol and is coupled to the CK19 monoclonal antibody. The preparation method comprises the following steps of performing a mixed reaction on the CK19 monoclonal antibody with the 2-mercaptoethanol, adding micromolecular dextran to be uniformly mixed, adding sodium glucoheptonate, stannous chloride and sodium pertechnetate for a reaction, and performing separation by adopting a PD-10 column. The tracer prepared by the method can effectively reduce local trauma of a patient by detecting the metastasis conditionof sentinel lymph nodes in vivo, provides a favorable decision basis for surgeons, and shortens the operation time of the patient.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a radionuclide-targeted breast cancer sentinel lymph node metastasis tracer and a preparation method. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] At present, detecting the metastatic status of sentinel lymph nodes in breast cancer requires the use of sentinel lymph node tracers to locate the sentinel lymph nodes and then remove them, and perform pathological analysis, molecular pathological examination or cytological detection on the isolated sentinel lymph nodes. However, the inventors of the present invention found that the sentinel lymph node tracers (methylene blue, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/10A61K103/10
CPCA61K51/1045
Inventor 丛斌斌王永胜曹晓珊孙晓蓉刘治国高永生李孟柯刘雁冰赵桐周鹏鹏吴爽梁颖
Owner 王永胜
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com