Breast cancer sentinel lymph node metastasis in-vivo fluorescence targeting tracer agent and preparation method thereof

Abstract

The invention provides a breast cancer sentinel lymph node metastasis in-vivo fluorescence targeting tracer agent and a preparation method thereof. The targeting tracer agent comprises a cytokeratin 19 monoclonal antibody and Cy754, epsilon-amino of lysine in the cytokeratin 19 monoclonal antibody and carboxyl of the Cy754 form amido bonds to be combined, and one CK19 monoclonal antibody moleculeis connected to 8-12 Cy754 molecules. The preparation method comprises the following steps: adding 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride into a mixed solution of the CK19 monoclonal antibody and Cy754, carrying out a reaction, and carrying out dialysis purification on the material obtained after the reaction to obtain the tracer agent. According to the tracer agent providedby the invention, when the region is detected through the photoacoustic conversion detector, the lymph node subjected to fluorescence targeting development can be found, so that the purpose of determining sentinel lymph node metastasis in vivo is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to an in vivo fluorescence targeting tracer for breast cancer sentinel lymph node metastasis and a preparation method. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The sentinel lymph node is a special lymph node in the lymph nodes of the primary tumor drainage area, and it is the first lymph node that the primary tumor must pass through for lymph node metastasis. The sentinel lymph node of breast cancer is the first lymph node where breast cancer cells metastasize through the lymphatic channel. During the segmental mastectomy plus sentinel lymph node biopsy, a small incision...

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Problems solved by technology

However, after research by the inventors, it has been found that the tracers of these detection methods only have the function of locating the sentinel lymph nodes, but do not have the function of indicating whether there are metastases. Increased workload. At the same time, intraoperative rapid frozen pathological examination and postoperative routine pathological diagnosis of sentinel lymph nodes will take a long time, which will affect the surgeon's judgment on the patient's condition

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