Method for clinically improving breast cancer by TIL

A breast cancer and tumor infiltration technology, applied in the field of immune cell therapy, can solve the problems of killing target cells and short lifespan of T cells

Pending Publication Date: 2020-10-30
卡替(上海)细胞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Effector T cells have a short lifespan and have the function of killing target cells

Method used

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Embodiment Construction

[0012] The present invention will be described in detail below in conjunction with specific embodiments.

[0013] The present invention discloses a method for clinically improving breast cancer by TIL. The specific operation steps of the method are: firstly remove the tumor from the patient; then the tumor tissue is transported to the laboratory under sterile, cold chain conditions, and is treated with machinery and enzymes. The digestion method is to separate the tumor infiltrating lymphocytes from the tumor, and separate the initial tumor infiltrating lymphocytes; add high-dose IL-2 in vitro culture and transformation, and expand the initial tumor infiltrating lymphocytes 10,000 times and further purify them. And cryopreservation; finally, the tumor-infiltrating lymphocytes are returned to the body of the patient with cholangiocarcinoma, and the average cell volume of tumor infiltrating lymphocytes per injection is 1×10 9 -5×10 10 One. The method for clinically improving breast...

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Abstract

The invention discloses a method for clinically improving breast cancer through TIL. The method comprises the steps that firstly, excising a tumor from the body of a patient; then transporting the tumor tissue to a laboratory under sterile and cold-chain conditions, locally separating tumor infiltrating lymphocytes from the tumor by using a mechanical treatment and enzyme digestion method, and separating to obtain initial tumor infiltrating lymphocytes; adding high-dose IL-2 for in-vitro culture and transformation, amplifying the initial tumor infiltration lymphocyte by ten thousand times, further purifying, and performing cryopreservation; finally, carrying out autotransfusion of the tumor infiltrating lymphocytes into the body of the biliary duct cancer patient, wherein the number of injected into the tumor infiltrating lymphocytes each time is 5 * 10 < 9 > to 5 * 10 < 10 >. According to the invention, lymphocytes in breast tumor tissues are separated and amplified, and then are fedback to the patient, so that the purpose of improving the living quality of the breast cancer patient can be achieved. The TIL tumor infiltration lymphocyte can recognize T cells of tumor cell neoantigens and has the capacity of efficiently killing tumor cells.

Description

Technical field [0001] The invention belongs to the technical field of immune cell therapy, and specifically relates to a method for clinically improving breast cancer with TIL. Background technique [0002] Breast cancer has become a major public health problem in the current society. The cause of breast cancer is not yet fully understood. Studies have found that there is a certain regularity in the incidence of breast cancer. Women with high risk factors for breast cancer are more likely to develop breast cancer. The so-called high-risk factors refer to various risk factors related to the onset of breast cancer, and the risk factors that most breast cancer patients have are called high-risk factors of breast cancer. According to the Chinese Cancer Registry Annual Report, the age-specific incidence of breast cancer in women is at a low level in the age range of 0-24 years old, gradually rising after 25 years old, reaching a peak at 50-54 years old, and gradually decreasing after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/17A61P35/00C12N5/0783
CPCA61K35/17A61P35/00C12N5/0636C12N2509/00C12N2509/10
Inventor 李军
Owner 卡替(上海)细胞生物技术有限公司
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